Mass spectrometric identification of adenosine 3′:5′-cyclic monophosphate isolated from a higher plant tissue

Newton, Russell P.; Gibbs, Neil K.; Moyse, Christopher D.; Wiebers, J. L.; Brown, Eric G.

doi:10.1016/0031-9422(80)83002-0

Cited by 47 publications

(14 citation statements)

References 8 publications

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“…Affi-Gel 10 and Dowex AG 50W-X4 were from in either buffer B or 0.1 M MOPS buffer, in the presence Bio-Rad (U.S.A.). [2, H]Adenosine 3:5-monophosof 0.02% NaN 3 . phate (1.55 TBq/mmol) and [2,5,8-3 H]adenosine 5-triphosphate (1.48 TBq/mmol) were obtained from Amersham International (UK).…”

Section: Methodsmentioning

confidence: 99%

Analysis of 3′,5′-cAMP and Adenylyl Cyclase Activity in Higher Plants Using Polyclonal Chicken Egg Yolk Antibodies

Roef

Witters

Gadeyne

et al. 1996

Analytical Biochemistry

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Section: Methodsmentioning

confidence: 99%

Analysis of 3′,5′-cAMP and Adenylyl Cyclase Activity in Higher Plants Using Polyclonal Chicken Egg Yolk Antibodies

Roef

Witters

Gadeyne

et al. 1996

Analytical Biochemistry

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“…It was mass spectrometric analysis that unequivocally established cAMP as being endogenous to plant tissues (Newton et al, 1980 ;Johnson et al, 1981 ;Janistyn, 1983). These first observations were obtained by electron-impact GC-MS of volatile trimethylsilyl derivatives of cAMP, as reported by Lawson et al (1971) with the chemically synthesized compound.…”

Section:       C   mentioning

confidence: 95%

Tansley Review No. 106

et al. 1999

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 For three decades, hypotheses relating to the occurrence and function of cyclic nucleotides in higher plants have been highly controversial. Although cyclic nucleotides had been shown to have key regulatory roles in animals and bacteria, investigations with higher plants in the 1970s and early 1980s were criticized on the basis of (i) a lack of specificity of effects apparently elicited by cyclic nucleotides, (ii) the equivocal identification of putative endogenous cyclic nucleotides and (iii) ambiguity in the identification of enzymes connected with cyclic nucleotide. More recent evidence based on more rigorous identification procedures has demonstrated conclusively the presence of cyclic nucleotides, nucleotidyl cyclases and cyclic nucleotide phosphodiesterases in higher plants, and has identified plant processes subject to regulation by cyclic nucleotides. Here we review the history of the debate, the recent evidence establishing the presence of these compounds and their role ; future research objectives are discussed.

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“…Convincing data have been obtained, which are related to the main components of the scrutinized system: cAMP, adenylate cyclase, phosphodiesterase, cAMP-binding proteins (Brown et al, 1980;Polya and Bowman, 1981;Phedenko et al, 1983;Yavorskaya and Kalinin, 1984;Tarchevsky, 2001), nucleotide-gated channels (Martinez-Atienza et al, 2007). The concentration of the endogenous cAMP is an indicator of the functional activity for this signaling.…”

Section: Introductionmentioning

confidence: 99%

“…On the basis of a decrease in the radioactivity observed, is make conclusion about the amount of the bound antigen. More rarely the following complex and labour-consuming methods are applied: mass spectrometry and high-performance gas-liquid chromatography (Newton et al, 1980), and also the bioluminescent method allowing to monitor the dynamics of cAMP in the living cell (Nicolaev and Lohse, 2006). Application of the all mentioned methods, in general, requires multi-step purification with the use of different ion-exchange resins, high voltage electrophoresis that can result in partial loss of the cyclic nucleotide.…”

Section: Introductionmentioning

confidence: 99%