Abstract:Abstract-The individual, deuterated, isomeric a-and p-carotenes were isolated from the green alga, Scenedesmus obliquus, cultivated in D,O containing 99.7 to 99.8 atom percent deuterium. Mass spectroscopy showed that both the a-and P-deuterio-carotene preparations contained principally the fully deuterated pigment molecules (C40D56), small quantities of deuterated molecules with one proton (C,,D,,H) and yet smaller quantities of deuterated molecules with two protons (C40D54H,). From statistical calculations th… Show more
A compilation of selected references to the use of deuterium in biochemical, pharmacological, clinical and environmental applications for the period 1971-1976 is presented. As a further aid, author and subject indices have been compiled.
A compilation of selected references to the use of deuterium in biochemical, pharmacological, clinical and environmental applications for the period 1971-1976 is presented. As a further aid, author and subject indices have been compiled.
This report presents analytical methods for the isolation and quantification of all-trans-beta-carotene-d8 in human plasma following a 73 mumol oral dose. Retinol-d4 derived from beta-carotene-d8 was also determined in the same plasma. Plasma samples drawn over a 24 day period were analyzed. beta-Carotene and retinol were isolated and purified for analysis using a solid phase extraction protocol with aminopropyl-bonded silica sorbent. Ratios of beta-carotene-d8/beta-carotene were determined using reversed-phase HPLC with spectrophotometric detection, which fully resolved the isotopomers, and by tandem mass spectrometry (MS/MS) with electron ionization. Results obtained from MS/MS and HPLC analysis showed close agreement and demonstrated improved selectivity relative to analysis using a single mass analyzer. Retinol-d4 was converted to its tert-butyldimethylsilyl ether and analyzed by gas chromatography/mass spectrometry using selected ion monitoring. The ability to resolve the beta-carotene isotopomers by HPLC makes it feasible for investigators without mass spectrometers to investigate the dynamics of absorption and metabolism of beta-carotene-d8 in humans.
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