Mass spectra of puromycin and some derivatives

Identification and characterization of a drug‐sensitive strain enables puromycin‐based translational assays in Saccharomyces cerevisiae

“…Subjecting OP‐puromycin to CID, the base peak of the tandem mass spectrum corresponds to loss of the dimethyl–aminopurine ring (see supporting information, Figure S3C). This reflects what is known concerning the mass spectrometric fragmentation of puromycin and related compounds, where a common fragment ion forms due to loss of the purine ring from the sugar (Eggers et al , ). Tandem MS of the preconjugated synthetic peptide resulted in observation of the b‐ and y‐series fragment ions common to peptides subjected to CID, and which correspond well to the consensus fragmentation pattern reported in the PeptideAtlas for the native peptide (see supporting information, Figure S3D).…”

Conformation of pyridine nucleotides studied by phosphorus-31 and hydrogen-1 fast Fourier transform nuclear magnetic resonance spectroscopy. III. Oxidized and reduced dinucleotides

“…The two phosphate dianion groups, each at the 2' positions of the adenine riboses, are as far apart as possible to minimize electrostatic repulsion. (2) The spatial orientation of the two adenine moieties is such that each affects the protons on the other in an identical manner.…”

2'-0-(α-Methoxyethyl)nucleoside 5'-diphosphates as single-addition substrates in the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase

“…These spectra have now been compared with those obtained from the trimethylsilyl derivatives of IVa and IVb synthesized from 3',5'-di-0-acetyladenosine. In the use of mass spectra for the determination of structures of CDsubstituted nucleosides the most useful fragment ion is that which contains the base, C-l', and C-2' together with any substituent located at C-2' (Biemann and McCloskey, 1962;Eggers et al, 1966;Shaw et al, 1970). Thus, the spectrum of the trimethylsilyl derivative of the nucleoside prepared by dephosphorylation of II indicated the ion, mje 235, that corresponds to this fragment, containing, in this case, a methoxyethyl group at C-2'.…”