Mass Selective Ion Transfer and Accumulation in Ion Trap Arrays

Wang, Yuzhuo; Zhang, Xiaohua; Zhai, Yanbing; You, Jane; Fang, Xiang; Zhou, Mingfei; Deng, Yulin; Xu, Wei

doi:10.1021/ac502583b

Cited by 25 publications

(20 citation statements)

References 52 publications

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“…Details about the instrument setup could be found in Wang et al [39]. Briefly, a nano electrospray ionization source was used for sample ionization.…”

Section: Ms Instrumentationmentioning

confidence: 99%

Reducing Space Charge Effects in a Linear Ion Trap by Rhombic Ion Excitation and Ejection

Zhang¹,

Wang²,

Hu³

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Space charge effects play important roles in ion trap operations, which typically limit the ion trapping capacity, dynamic range, mass accuracy, and resolving power of a quadrupole ion trap. In this study, a rhombic ion excitation and ejection method was proposed to minimize space charge effects in a linear ion trap. Instead of applying a single dipolar AC excitation signal, two dipolar AC excitation signals with the same frequency and amplitude but 90°phase difference were applied in the xand y-directions of the linear ion trap, respectively. As a result, mass selective excited ions would circle around the ion cloud located at the center of the ion trap, rather than go through the ion cloud. In this work, excited ions were then axially ejected and detected, but this rhombic ion excitation method could also be applied to linear ion traps with ion radial ejection capabilities. Experiments show that space charge induced mass resolution degradation and mass shift could be alleviated with this method. For the experimental conditions in this work, space charge induced mass shift could be decreased by~50%, and the mass resolving power could be improved by~2 times at the same time.

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“…Details about the instrument setup could be found in Wang et al [39]. Briefly, a nano electrospray ionization source was used for sample ionization.…”

Section: Ms Instrumentationmentioning

confidence: 99%

Reducing Space Charge Effects in a Linear Ion Trap by Rhombic Ion Excitation and Ejection

Zhang¹,

Wang²,

Hu³

et al. 2016

J. Am. Soc. Mass Spectrom.

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“…It may be a convenient instrument for studying top-down proteomics to detect intact proteins. Wang et al used the first ion trap to store the injected ions (Wang et al, 2014). The targeted ions were then selectively ejected into the second ion trap.…”

Section: Combination Of Ion Trap and Other Analyzers Ion Trap Arraysmentioning

confidence: 99%

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

Li¹,

Chu²,

Tan³

et al. 2021

Front. Chem.

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Mass spectrometry (MS) is one of the most widely used analytical techniques in many fields. Recent developments in chemical and biological researches have drawn much attention to the measurement of substances with low abundances in samples. Continuous efforts have been made consequently to further improve the sensitivity of MS. Modifications on the mass analyzers of mass spectrometers offer a direct, universal and practical way to obtain higher sensitivity. This review provides a comprehensive overview of the latest developments in mass analyzers for the improvement of mass spectrometers’ sensitivity, including quadrupole, ion trap, time-of-flight (TOF) and Fourier transform ion cyclotron (FT-ICR), as well as different combinations of these mass analyzers. The advantages and limitations of different mass analyzers and their combinations are compared and discussed. This review provides guidance to the selection of suitable mass spectrometers in chemical and biological analytical applications. It is also beneficial to the development of novel mass spectrometers.

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“…For example, ion mobility-mass spectrometry (IM-MS) has been shown to improve sensitivity and selectivity by fractionating mixtures on the basis of ion collisional cross section (CCS).24-26 Other approaches have taken advantage of hybrid mass spectrometry instrumentation to perform ion accumulation in a separate ion trap prior to mass analysis. 27 For example, ion accumulation in a linear octopole ion trap positioned external to a Fourier transform ion cyclotron resonance (FTICR) cell enabled dramatic improvements in sensitivity.28 29 These external accumulation setups have demonstrated limits of detection of 20 zmol of cytochrome c and duty cycles of 100% during electrospray ionization (ESI) experiments.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Range Expansion by Gas-Phase Ion Fractionation and Enrichment for Imaging Mass Spectrometry

Prentice

Ryan²,

Grove³

et al. 2020

Preprint

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In the analysis of biological tissue by imaging mass spectrometry (IMS), the limit of detection and dynamic range are of paramount importance in obtaining experimental results that provide insight into underlying biological processes. Many important biomolecules are present in the tissue milieu in low concentrations and in complex mixtures with other compounds of widely ranging abundances, challenging the limits of analytical technologies. In many IMS experiments, the ion signal can be dominated by a few highly abundant ion species. On trap-based instrument platforms that accumulate ions prior to mass analysis, these high abundance ions can diminish the detection and dynamic range of lower abundance ions. Herein, we characterize two strategies for combating these challenges during IMS experiments on a hybrid QqFT-ICR MS. In one iteration, the mass resolving capabilities of a quadrupole mass filter are used to selectively enrich for ions of interest via a technique previously termed continuous accumulation of selected ions (CASI). Secondly, we have introduced a supplemental dipolar AC waveform to the quadrupole mass filter of a commercial QqFT-ICR mass spectrometer to perform selected ion ejection prior to the ion accumulation region. This setup allows the selective ejection of the most abundant ion species prior to ion accumulation, thereby greatly improving the molecular depth with which IMS can probe tissue samples.<br>

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Mass Selective Ion Transfer and Accumulation in Ion Trap Arrays

Cited by 25 publications

References 52 publications

Reducing Space Charge Effects in a Linear Ion Trap by Rhombic Ion Excitation and Ejection

Reducing Space Charge Effects in a Linear Ion Trap by Rhombic Ion Excitation and Ejection

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

Dynamic Range Expansion by Gas-Phase Ion Fractionation and Enrichment for Imaging Mass Spectrometry

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