Mass-Rearing of the Larvae of Nine Noctuid Species on a Simple Artificial Medium12

Shorey, H. H.; Hale, R. L.

doi:10.1093/jee/58.3.522

Cited by 970 publications

(443 citation statements)

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“…Both colonies were kept in BOD-type incubators under a 14:10 (L:D) photoperiod, 21-23°C and 60-70% RH. The larvae were reared either in glass tubes or in plastic cups on an artifi cial diet based on bean homogenate and agar adapted from (Shorey & Hale 1965), by using butter and back beans for the Uruguayan and Brazilian cultures, respectively. Virgin adults were obtained by separating male and female pupae.…”

Section: Methodsmentioning

confidence: 99%

Reproductive behaviour of Crocidosema (=Epinotia) aporema (Walsingham) (Lepidoptera: Tortricidae): temporal pattern of female calling and mating

Altesor¹,

Horas²,

Arcia³

et al. 2010

Neotrop. entomol.

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-Crocidosema aporema (Walsingham) is a major pest of legumes in the southern cone of Latin America. The mating behaviour of two allopatric populations (Uruguay and Brazil) of C. aporema kept in captivity was studied by observing the posture of calling females, the temporal pattern of pheromone emission and mating, and the response of males to calling females in olfactometer tests. Female calling and mating was observed during the scotophase, from the fi rst to the seventh night after adult emergence. Male response was evaluated at night using a single calling female in a Y-shaped olfactometer. Females adopted a characteristic calling posture, extruding the pheromone gland from the tip of the abdomen. Most females started calling during the second scotophase, and all females called from the third, between the fi fth and seventh hours after the onset of the scotophase. Most of the couples mated once throughout the experiment, between the third and sixth night and during the middle of the dark phase. Males preferentially chose the female arm in olfactometer tests, considering both the fi rst arm chosen and the number of visits during the observation period. Our results describe for the fi rst time the temporal pattern associated to the reproductive behaviour of C. aporema. We also provide evidence that this tortricid is monoandrous, and that pheromones are used in intersexual communication for mate fi nding. Our data will be used to optimize the collection of female sex pheromones for chemical characterization in order to develop a monitoring tool for this pest.

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Section: Methodsmentioning

confidence: 99%

Reproductive behaviour of Crocidosema (=Epinotia) aporema (Walsingham) (Lepidoptera: Tortricidae): temporal pattern of female calling and mating

Altesor¹,

Horas²,

Arcia³

et al. 2010

Neotrop. entomol.

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“…Heliothis virescens larvae were reared on a modified pinto bean diet [28]. Eggs and larvae of both insect species were kept at 27 °C on a 16 h:8 h L:D cycle.…”

Section: Experimental Animalsmentioning

confidence: 99%

The pheromone biosynthesis activating neuropeptide (PBAN) receptor of Heliothis virescens: Identification, functional expression, and structure–activity relationships of ligand analogs

et al. 2008

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Pheromone biosynthesis activating neuropeptide (PBAN) promotes synthesis and release of sex pheromones in moths. We have identified and functionally expressed a PBAN receptor from Heliothis virescens (HevPBANR) and elucidated structure-activity relationships of PBAN analogs. Screening of a larval CNS cDNA library revealed three putative receptor subtypes and nucleotide sequence comparisons suggest they are produced through alternative splicing at the 3'-end. RT-PCR amplified preferentially HevPBANR-C from female pheromone glands. CHO cells expressing HevPBANR-C are highly sensitive to PBAN and related analogs, especially those sharing the C-terminal pentapeptide core, FXPRLamide (X = T, S or V). Alanine replacements in the C-terminal hexapeptide (YFTPRLamide) revealed the relative importance of each residue in the active core as follows, R 5 >L 6 >F 2 ≫P 4 >T 3 ≫Y 1 . This study provides a framework for the rational design of PBANR-specific agonists and/or antagonists that could be exploited for disruption of reproductive function in agriculturally important insect pests.

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“…Moths from North Carolina were supplied by Benzon Research (Carlisle, PA, USA). Moths were reared at 24-C and 60% RH on pintobean-based diet (Shorey and Hale, 1965) under a 16:8 (L/D) photoperiod. Pupae were sorted by sex, and adults emerged in 1-l plastic cages containing 5% sucrose in plastic cups with cotton dental wicks protruding from their lids.…”

Section: Insect Coloniesmentioning

confidence: 99%

Orientational Behaviors and EAG Responses of Male Codling Moth After Exposure to Synthetic Sex Pheromone from Various Dispensers

2006

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The effect of brief pheromone exposures on responses of codling moth (Cydia pomonella L.) males was tested by flight-tunnel and electroantennogram (EAG) studies. Males were preexposed to pheromone for up to 3 min as they sat in release cages or for shorter times (a few seconds to several min) upon initiating flights or orienting in plumes. Brief exposures to Isomate-C Plus dispensers nearly eliminated moth orientations to 0.1 mg codlemone [(E,E)-8,10-dodecadien-1-ol] and 0.1 mg three-component (codlemone/12OH/14OH, 100:20:5 ratio) lures 15 min later. However, there was no associated change in EAG responses between preexposed and control moths. Behavioral responses of Isomate-C Plus-exposed males were normal 24 hr following exposure. The reduced sexual responsiveness observed following exposure to Isomate dispensers appeared to be associated with an elevation of response threshold. Brief preexposure to 0.1 mg codlemone and three-component lures also reduced orientational behavior of males 15 min later, but to a lesser degree than when preexposed to Isomate-C Plus dispensers. Male behavior following preexposure to a 0.1 mg codlemone/pear ester [(2E,4Z)-2,4-decadienoate] lure (1:1 ratio) was no different from exposure to codlemone only. Orientational disruption in plots treated with 10 dispensers of Isomate-C Plus per tree was 88.3 and 95.9% for 1.0 and 0.1 mg codlemone lures, respectively. Some males did orient to 0.1 mg codlemone lures so we caution that flight-tunnel experiments on preexposure may overestimate the actual pheromone exposure dosage received by feral moths in treated orchards. Importantly, this work documents that a portion of feral males within a population has the capacity to overcome communicational disruption by high densities of Isomate-C Plus dispensers.

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Mass-Rearing of the Larvae of Nine Noctuid Species on a Simple Artificial Medium12

Cited by 970 publications

References 0 publications

Reproductive behaviour of Crocidosema (=Epinotia) aporema (Walsingham) (Lepidoptera: Tortricidae): temporal pattern of female calling and mating

Reproductive behaviour of Crocidosema (=Epinotia) aporema (Walsingham) (Lepidoptera: Tortricidae): temporal pattern of female calling and mating

The pheromone biosynthesis activating neuropeptide (PBAN) receptor of Heliothis virescens: Identification, functional expression, and structure–activity relationships of ligand analogs

Orientational Behaviors and EAG Responses of Male Codling Moth After Exposure to Synthetic Sex Pheromone from Various Dispensers

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