Mass Propagation of Robusta Clones: Disposable Plastic Bags for Pregermination of Somatic Embryos by Temporary Immersion

Werner

Engineering in Life Sciences

2009

Driven by the demands of the market and the manufacturing industry, disposable bioreactors have gained in importance in cell culture‐based processes during the last 10 years. Today they are widely accepted in R&D and also in manufacturing where process simplicity, safety and flexibility have top priority. Although disposable bioreactors are mainly used for cell expansions, glycoprotein secretions and virus generations realised with mammalian and insect cell lines, there are several reports delineating their suitability for the cultivation of plant cell and tissue cultures. This review describes the current disposable bioreactor types suitable for growing plant cell suspensions and organ cultures (hairy roots, meristematic clusters, somatic embryos) at Litre‐scale. Based on a definition of the term “disposable bioreactor”, a categorisation of the prevalent types for plant liquid cultures is presented. We describe the bioreactor regimes, working principles and bioengineering parameters of mechanically and pneumatically agitated bag bioreactors, which have advantages of process scalability and efficiency. Furthermore, results from the literature and data from our own research (obtained during production of undifferentiated bioactive cells, expressions of secondary metabolites and glycoproteins, and micropropagations of plant tissues) are discussed.

Section: Special Case: Box‐in‐bag Bioreactormentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Disposable bioreactors for plant liquid cultures at Litre‐scale

Werner

Engineering in Life Sciences

2009

“…One improvement would be to increase the surface-tovolume ratio; one possibility is to place a rigid box inside a plastic bag. We presented this new TIB, so-called ''Box-in-Bag'' in the 27th International Horticultural Congress (Séoul, August 2006) (Ducos et al 2007c). This experimental culture system is sterilized by gamma radiation and connected to the usual 5-l medium bottle (Fig.…”

Section: Bioreactors For the Production Of Somatic Embryosmentioning

confidence: 99%

Improvement of plastic-based disposable bioreactors for plant science needs

et al. 2008

Self Cite

The present article describes two new applications of plastic-based cell culture systems in the plant biotechnology domain. Different types of bioreactors are used at Nestlé R&D Center-Tours for large scale culture of plants cells to produce metabolites or recombinant proteins and for mass propagation of selected plant varieties by somatic embryogenesis. Particularly, recent studies are directed to cut down the production costs of these two processes by developing disposable cell culture systems. For large scale culture, two novel flexible plastic-based disposable bioreactors have been devel-

In Vitro Cell.Dev.Biol.-Plant

“…The production of cassava plants from milligrams of embryogenic cells could become much more efficient if, for example, liquid (RITA®) instead of solid media were used, facilitating the synchronization of somatic embryo development. The automation of somatic embryo production using liquid systems in bioreactors requires standard environmental conditions like pH, temperature, light, O 2 supply, CO 2 exchange, media composition (i.e., growth regulators), and antibiotic depletion, among other variables (Ducos et al 2007 ). Coffee is an excellent example: bioreactors facilitate the massive production of 2–4 × 10 5 somatic embryos in 2–5 l bioreactors for direct sowing in the greenhouse, without encapsulation.…”

Section: Introductionmentioning

confidence: 99%

“…Coffee is an excellent example: bioreactors facilitate the massive production of 2–4 × 10 5 somatic embryos in 2–5 l bioreactors for direct sowing in the greenhouse, without encapsulation. This approach is worthwhile trying for cassava (Ducos et al 2007 ; 2011 ; Etienne et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

The potential of using biotechnology to improve cassava: a review

Chavarriaga-Aguirre

Brand

Medina

et al. 2016

The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a better crop for a constantly changing world. We therefore review the state of knowledge on the current use of biotechnology applied to cassava cultivars and its implications for breeding the crop into the future. The history of the development of the first transgenic cassava plant serves as the basis to explore molecular aspects of somatic embryogenesis and friable embryogenic callus production. We analyze complex plant-pathogen interactions to profit from such knowledge to help cassava fight bacterial diseases and look at candidate genes possibly involved in resistance to viruses and whiteflies—the two most important traits of cassava. The review also covers the analyses of main achievements in transgenic-mediated nutritional improvement and mass production of healthy plants by tissue culture and synthetic seeds. Finally, the perspectives of using genome editing and the challenges associated to climate change for further improving the crop are discussed. During the last 30 yr, great advances have been made in cassava using biotechnology, but they need to scale out of the proof of concept to the fields of cassava growers.