Mass Production of Genetically Modified &lt;em&gt;Aedes aegypti&lt;/em&gt; for Field Releases in Brazil

Carvalho, Danilo O.; Nimmo, Derric; Naish, Neil; McKemey, Andrew R.; Gray, Pam; Wilke, André Barretto Bruno; Marrelli, Mauro Toledo; Virgínio, Jair Fernandes; Alphey, Luke; Capurro, Margareth Lara

doi:10.3791/3579

Cited by 112 publications

(120 citation statements)

References 34 publications

(33 reference statements)

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“…There have been some recent attempts to study male mating ability in dengue vectors (Oliva et al, 2012;Dieng et al, 2013), but these studies did not involve Ae. aegypti, a species that is currently the target of SIT and GMM operations in many parts of the world (Harris et al, 2012;Carvalho et al, 2014). Another recent study examined mating success in this species, taking into account body size (Ponlawat & Harrington, 2009), but the authors did not focus on male size and reproductive outcome, i.e., egg production, a prerequisite for the production of subsequent generations and a determinant of population maintenance.…”

Section: Introductionmentioning

confidence: 99%

Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae)

Dieng

Abang

Ahmad

et al. 2016

J Entomol Acarol Res

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Body size is a physical factor of crucial importance underlying important traits of the reproductive dynamics of both sexes in mosquitoes. Most studies on the influence of body size in mating success of dengue vectors addressed sperm transfer to females and did not consider egg production, a prerequisite for population maintenance; male body size impact on reproduction has attracted little research interest with respect to sterile insect technique. In experiments involving differently sized adults, we examined whether the body size of the mates is a source of variation in reproductive outcome in Aedes aegypti. In the absence of male partners, large females (LF) showed better fecundity than small females (SF). In intraclass mating trials, egg production was much greater in largesized than smallsized pairs. There were comparable fecundities in large females mated with small males and large pairs. [SF•SM] and [SF•LM] pairs showed equivalent fecundity. Nonmating did not result in the production of viable eggs by either small or large females. We also observed that eggs produced by largesized females mated with small males had better hatching success than those from either small or large pairs. Mating between small females and large males resulted in poor egg viability.

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Section: Introductionmentioning

confidence: 99%

Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae)

Dieng

Abang

Ahmad

et al. 2016

J Entomol Acarol Res

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“…aegypti are proposed using sterilized or genetically modified males being released to mate with native females. [1][2][3] Understanding the details of the mating behavior of this vector is critical, both in terms of understanding the population genetics of the insect and increasing the effectiveness of potential genetic control programs.…”

Section: Introductionmentioning

confidence: 99%

Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Richardson¹,

Jameson²,

Gloria‐Soria³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The mosquito Aedes aegypti is a vector of yellow fever, dengue, and chikungunya. Control of the insect is crucial to stop the spread of dengue and chikungunya, so it is critically important to understand its mating behavior. Primarily, based on laboratory behavior, it has long been assumed that Ae. aegypti females mate once in their lifetime. However, multiple inseminations have been observed in semi-field and laboratory settings, and in closely related species. Here, we report the first evidence of polyandry in a natural population of Ae. aegypti. Female Ae. aegypti were captured around the New Orleans, LA, metropolitan area. They were offered a blood meal and allowed to lay eggs, which were reared to the third-instar larval stage. A parentage analysis using four microsatellite loci was performed. Out of 48 families, 3 showed evidence of multiple paternity. An expanded analysis of these three families found that one family group included offspring contributed by three fathers, and the other two included offspring from two fathers. This result establishes that polyandry can occur in a small proportion of Ae. aegypti females in a natural setting. This could complicate future genetic control efforts and has implications for sampling for population genetics.

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“…It may be possible to obtain sufficient virgin females through mechanical sorting of males and females at an early stage of development. Sorting could be based on differences in size between the sexes (Carvalho et al 2014) or sex-specific expression of a fluorescent marker gene (Catteruccia, Benton, and Crisanti 2005).…”

Section: Gene Drive Systems and Agricultural Insect Pestsmentioning

confidence: 99%

Agricultural production: assessment of the potential use of Cas9-mediated gene drive systems for agricultural pest control

Scott

Gould

Lorenzen

et al. 2017

Journal of Responsible Innovation

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Mass Production of Genetically Modified <em>Aedes aegypti</em> for Field Releases in Brazil

Cited by 112 publications

References 34 publications

Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae)

Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae)

Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Agricultural production: assessment of the potential use of Cas9-mediated gene drive systems for agricultural pest control

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