Mass production of fertilized eggs by artificial insemination from captive-reared Pacific bluefin tuna (Thunnus orientalis)

Endo, Taku; Ishida, Masashi; Yazawa, Ryosuke; Takeuchi, Yutaka; Kumakura, Naoki; Hara, Takashi; Adachi, Souichi

doi:10.1016/j.aquaculture.2015.08.035

Cited by 10 publications

(8 citation statements)

References 13 publications

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“…However, it is difcult to perform temperature treatment on PBT juveniles at the gonadal sex diferentiation stage since they are generally reared in ofshore sea net cages, as discussed above. Furthermore, it is difcult to perform artifcial insemination in mature PBT bloodstocks for producing crossspecies hybrids, owing to their large body size and very delicate skin, which can be easily damaged [21]. Hence, our results indicate that oral AI administration is the most effective and practical method for inducing masculinization in genotypically female PBT in an aquaculture.…”

Section: Discussionmentioning

confidence: 85%

“…Several methods have been developed for administering sex steroids and AI, including direct injection, direct immersion, and dietary supplementation [26]. However, direct injection is difcult in PBT because it is very difcult to handle this fsh, which have extremely delicate skin that can be easily damaged [21]. Furthermore, PBT juveniles at the stage of gonadal sex diferentiation (approximately 40−70 dph) [30] are generally reared in ofshore sea net cages, which helps avoid death due to collision with the walls of the indoor tank in aquaculture operations [2].…”

Section: Discussionmentioning

confidence: 99%

“…Tese fndings potentially suggest that in tuna species, males also exhibit higher growth performance compared to females in aquacultural settings. Long periods of time (approximately, more than 3 years) and huge economic costs are required to rear PBT until harvest size [8,21]. If male-cultured PBT exhibit higher growth performance than females, the production of mono-sex male stocks seems to act as a prominent tool in signifcantly improving the productivity of closed-cycle PBT aquaculture by shortening production times and reducing production costs.…”

Section: Introductionmentioning

confidence: 99%

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First Data on Sexual Dimorphic Growth of Cultured Pacific Bluefin Tuna, Thunnus orientalis (Temminck et Schlegel), and Its Sex Manipulation by Treatment with an Aromatase Inhibitor

Hayashida

Higuchi

Okita³

et al. 2023

Aquaculture Research

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Tuna (genus Thunnus), particularly Pacific bluefin tuna (T. orientalis; PBT), are commercially important fish in the aquaculture industry worldwide. The objective of this study was to investigate sexual dimorphism in the growth performance of aquaculture-produced PBT and develop techniques for its sex manipulation, for the first time in tuna. A comparison of the body size between sexes revealed that male-cultured PBTs were larger than females at harvest. We also confirmed that cyp19a1a (encoding a gonadal aromatase) expression increased specifically in the genotypic female PBT gonads during sex differentiation. This suggested that aromatase plays an important role in ovarian differentiation and that the suppression of aromatase activity may effectively induce masculinization in genotypic females. Therefore, we administered letrozole—an aromatase inhibitor (AI)—into sexually undifferentiated PBT through the oral route. AI administration resulted in a 100% sex reversal of genotypic females into phenotypic males at the molecular level. Our results provide the basis for future studies on the establishment of mono-sex male production technology in PBT, which would help improve the productivity of closed-cycle PBT aquaculture. Furthermore, this study offers important insights into the understanding of the sex-wise growth of tuna species in aquacultural settings, and developing sex manipulation techniques.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First Data on Sexual Dimorphic Growth of Cultured Pacific Bluefin Tuna, Thunnus orientalis (Temminck et Schlegel), and Its Sex Manipulation by Treatment with an Aromatase Inhibitor

Hayashida

Higuchi

Okita³

et al. 2023

Aquaculture Research

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“…To date, genetic improvement in bluefin tuna species that have long generation times (3‒5 years) has been challenging because it takes a long time to produce F2 individuals of a single double allelic mutation 2 . In addition, technology for selective mating by artificial insemination has not been established because mature bluefin tuna are very difficult to handle owing to their large body size and very delicate skin, which can be easily damaged 24 . Our results demonstrated that the Platinum TALEN system induced site-specific mutation with high efficiency, suggesting that homozygous null mutants could be generated in the F1 generation by crossing between the TALEN-injected G0 founders 17 .…”

Section: Discussionmentioning

confidence: 99%

Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)

Higuchi

Kazeto

Ozaki

et al. 2019

Sci Rep

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In bluefin tuna aquaculture, high mortalities of hatchery-reared juveniles occur in sea cages owing to wall collisions that are caused by high-speed swimming in panic due to changes in illuminance. Here, we report that targeted gene mutagenesis of the ryanodine receptor (RyR1b), which allows the sarcoplasmic reticulum to release Ca2+ in fast skeletal muscle, using highly active Platinum TALENs caused slow swimming behaviour in response to external stimuli in Pacific bluefin tuna (PBT) larvae. This characteristic would be a useful trait to prevent wall collisions in aquaculture production. A pair of Platinum TALENs targeting exons 2 and 43 of the PBT ryr1b gene induced deletions in each TALEN target site of the injected embryos with extremely high efficiency. In addition, ryr1b expression was significantly decreased in the mutated G0 larvae at 7 days after hatching (DAH). A touch-evoked escape behaviour assay revealed that the ryr1b-mutated PBT larvae swam away much less efficiently in response to mechanosensory stimulation at 7 DAH than did the wild-type larvae. Our results demonstrate that genome editing technologies are effective tools for determining the functional characterization of genes in a comparatively short period, and create avenues for facilitating genetic studies and breeding of bluefin tuna species.

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“…Technical control of the whole breeding cycle for the bluefin tuna (Thunnus oreintalis) and European eel (Anguilla anguilla), despite significant progress, have yet to reach commercially viable levels (54,55) . The harvest of juvenile European eels attracts significant criticism and, as an endangered species, their harvest has been made illegal in the European Union.…”

Section: Wild and Farmed: The Linkagesmentioning

confidence: 99%

Aquaculture: a rapidly growing and significant source of sustainable food? Status, transitions and potential

2016

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The status and potential of aquaculture is considered as part of a broader food landscape of wild aquatic and terrestrial food sources. The rationale and resource base required for the development of aquaculture are considered in the context of broader societal development, cultural preferences and human needs. Attention is drawn to the uneven development and current importance of aquaculture globally as well as its considerable heterogeneity of form and function compared with established terrestrial livestock production. The recent drivers of growth in demand and production are examined and the persistent linkages between exploitation of wild stocks, full life cycle culture and the various intermediate forms explored. An emergent trend for sourcing aquaculture feeds from alternatives to marine ingredients is described and the implications for the sector with rapidly growing feed needs discussed. The rise of non-conventional and innovative feed ingredients, often shared with terrestrial livestock, are considered, including aquaculture itself becoming a major source of marine ingredients. The implications for the continued expected growth of aquaculture are set in the context of sustainable intensification, with the challenges that conventional intensification and emergent integration within, and between, value chains explored. The review concludes with a consideration of the implications for dependent livelihoods and projections for various futures based on limited resources but growing demand

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Mass production of fertilized eggs by artificial insemination from captive-reared Pacific bluefin tuna (Thunnus orientalis)

Cited by 10 publications

References 13 publications

First Data on Sexual Dimorphic Growth of Cultured Pacific Bluefin Tuna, Thunnus orientalis (Temminck et Schlegel), and Its Sex Manipulation by Treatment with an Aromatase Inhibitor

First Data on Sexual Dimorphic Growth of Cultured Pacific Bluefin Tuna, Thunnus orientalis (Temminck et Schlegel), and Its Sex Manipulation by Treatment with an Aromatase Inhibitor

Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)

Aquaculture: a rapidly growing and significant source of sustainable food? Status, transitions and potential

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