Mass production of animal cells in mice with retention of cell specific markers

Freedman, Victoria H.; Brown, André L.; Klinger, H.P.; Shin, Seung-Il

doi:10.1016/0014-4827(76)90473-0

Cited by 13 publications

(5 citation statements)

References 12 publications

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“…All of the cells derived from sub clones, subcolonies, and tumors were able to grow in medium containing ouabain and thioguanine, indicating that, throughout the many cell gen erations involved in the various selection procedures and growth in vivo, the cells retained their ouabain-resistant and HPRT-deficient phenotypes. This confirmed results of earlier studies, in which WOR-6 and other cell strains retained their biochemical and genetic markers when grown in the nude mouse (Freedman et al, 1976).…”

Section: Observationssupporting

confidence: 80%

“…In subsequent experiments, we observed that when an established line of near-diploid (l.v) Chinese hamster cells, which initially contained only about 10% 2s cells, were passaged several times in semisolid medium, or injected into nude mice, all or the majority of the cells of the resulting colonies or tumors had chromosome complements approaching the 2s value (Freedman et al, 1976). This report deals with more detailed analyses that confirm these observations.…”

supporting

confidence: 69%

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Enhanced anchorage independence and tumorigenicity of aneuploid Chinese hamster cells with nearly doubled chromosome complements

Klinger

Shin

Freedman

1976

Cytogenet Genome Res

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The role of a cell’s chromosome complement in its tumorigenic and anchorage-independent growth properties in vitro was investigated by injecting a Chinese hamster cell line and its subclones into immunodeficient nude mice and by plating the cells in a semisolid medium containing methylcellulose. The parental WOR-6 cell clone originally consisted of 89 % 1s cells and 11 % cells with a nearly double (2s) complement. Tumors that developed from WOR-6 were found to consist entirely or primarily of cells with near 2s chromosome complements. Subclones of WOR-6 that contained only 1s cells rarely produced tumors in nude mice, even at high inoculum doses, whereas clones containing a high fraction of 2s cells were consistently tumorigenic. The degree of cellular tumorigenicity thus correlated with cell ploidy. In addition, serial passage of WOR-6 cells in semisolid medium resulted in selective enrichment for near 2s cells and, concomitantly, greatly enhanced tumorigenicity. Analyses of G-banded chromosomes revealed that the 1s cells of the WOR-6 parental clone, which has a modal chromosome number of 21 and a range of 18 to 23, is completely or partially monosomic for some chromosomes and trisomic for others. The 2s cells, selected both in vivo through growth as tumors in nude mice and in vitro in semisolid medium, appeared to have resulted from preferential duplication of certain chromosomes of the 1s cells. Our results therefore suggest that cells which develop multiple copies of selected genes, while remaining functionally hemizygous for other loci, acquire an enhanced anchorage-independent growth potential in vitro and increased tumorigenicity. This conclusion is consistent with the observation that cellular tumorigenicity is correlated with anchorage independence (Freedman and Shin, 1974) and lends support to Ohno’s (1974)

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Section: Observationssupporting

confidence: 80%

supporting

confidence: 69%

Enhanced anchorage independence and tumorigenicity of aneuploid Chinese hamster cells with nearly doubled chromosome complements

Klinger

Shin

Freedman

1976

Cytogenet Genome Res

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“…cells in the nude mouse does not affect the electrophoretic mobilities of several other enzymes (50,51). Similar results have been observed for tumors in nude mice originating from cell lines of the Chinese and Syrian hamsters, mouse, rat, rabbit, and man, and with human-rodent cell hybrids (52)(53)(54)(55)(56).…”

Section: Constancy Of Phenotypes Shown Insupporting

confidence: 74%

Stability of Polymorphic Enzyme Phenotypes in Human Tumor Cell Lines

Wright

Daniels

Fogh

1979

Experimental Biology and Medicine

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“…Similarly, a choriocarcinoma synthesized HCG (Kamneya et al, 1976), and human mammary carcinomas continue to produce calcitonin (Coombes et al, 1975) and tumourassociated glycolipid (absent from nonmalignant tissue) and shed this into the serum (Nordquist et al, 1978). Similar studies with animal-tumour xenografts have demonstrated the synthesis of myeloma protein by a mouse plasmacytoma, insulin by a hamster pancreatic-islet-cell tumour, and SV40 T-antigen by SV40-transformed mouse, rat and rabbit cells (Freedman et al, 1976). The objective of the present studies was to examine the continued production in xenografts of the unique cell-surface tumour-associated neoantigens of 2 carcinogen-induced rat tumours, and here it has been demonstrated that these antigens are expressed during xenograft growth.…”

supporting

confidence: 53%

Serological aspects of rat tumour xenograft growth in athymic nude mice

Pimm¹,

Baldwin²

1979

Br J Cancer

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Summary.-The serum of athymic nude mice bearing rat tumour xenografts has been examined for tumour-specific antigen. With a sarcoma and a hepatoma, tumourspecific antigen expression continued in xenograft growths, and sera of tumourbearing mice contained free antigen, assayed by its ability to neutralise reactivity of tumour-immune rat sera against tumour target cells in an indirect membraneimmunofluorescence test. In contrast, no anti-rat antibody was detectable in sera of mice bearing the xenografts, or rejecting cells injected in admixture with BCG.

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Mass production of animal cells in mice with retention of cell specific markers

Cited by 13 publications

References 12 publications

Enhanced anchorage independence and tumorigenicity of aneuploid Chinese hamster cells with nearly doubled chromosome complements

Enhanced anchorage independence and tumorigenicity of aneuploid Chinese hamster cells with nearly doubled chromosome complements

Stability of Polymorphic Enzyme Phenotypes in Human Tumor Cell Lines

Serological aspects of rat tumour xenograft growth in athymic nude mice

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