Maspin expression and subcellular localization in invasive ductal carcinoma of the breast: Prognostic significance and relation to microvessel density

Abstract

Maspin (Mammary serine protease inhibitor) is a tumor suppressor serine. Its clinical significance and role in breast carcinoma are contradictory and inconclusive. Researches demonstrated that the function of maspin differs according to its subcellular localization. This study was conducted to investigate the expression of maspin in invasive ductal carcinoma (IDC) of the breast with special emphasis on its subcellular localization and to evaluate its prognostic role in relation to clinicopathological parameters and microvessel density (MVD) of the tumor. The expression of maspin was evaluated immunohistochemically in 45 IDC cases. The positive rate of maspin expression was 73.3%. Maspin positivity was significantly related to higher tumor grade (p value = 0.041), nodal metastasis (p value = 0.044), perineural invasion (p value = 0.047), and high CD34+MVD (p value = 0.002). Nuclear maspin was detected in 36.6% whereas cytoplasmic maspin was detected in 63.4% of maspin positive cases. A significant inverse relationship was observed between nuclear maspin and high tumor grade (p value = 0.016), and nodal metastasis (p value = 0.047). These results suggest that maspin expression has a prognostic role in breast cancer. Maspin expression is related to increased angiogenesis. Subcellular localization of maspin can strongly affect cancer prognosis. Cytoplasmic maspin relates to poor prognostic parameters whereas nuclear maspin relates to good prognostic ones.

The Role of Maspin Expression as Diagnostic Tissue Marker in Pancreaticoduodenal Malignant Tumors and Benign Lesions

“…A non-significant relationship was found between maspin distribution and N stage of malignant pancreatic epithelial tumor (p >0.05). Contrary to our results, Helal and El-Guindy, Terashima et al, and Oh et al [19], [22], [27] found significant relation between immunoreactivity for maspin and nodal metastasis. They noted that maspin expression was higher in lymph node-positive carcinomas than in lymph node-negative carcinomas.…”

“…Maspin positivity is inversely proportional to tumor grade, being more among poorly differentiated cases. These results were similar to that conducted on Maspin expression in breast carcinoma by Helal and El-Guindy, Lee et al, Umekita and Yoshida [22], [23], [24], [25] who reported a strong association between Maspin expression and higher histologic grade. On the other hand, Jason et al, [9] did not find a correlation between maspin staining and histologic grade of the pancreatic tumors.…”

Placental Maspin Gene Expression Pattern May Be Used to Distinguish Between Preeclampsia and Intrauterine Growth Restriction

“…Maspin which is a tumor suppressor gene and inhibits invasion, motility, and metastasis of tumor cells and neoangiogenesis, is one of these genes (11). Maspin expression was downregulated via promoter methylation and this effect were studied previously in different cancer type (19,20). It has been also reported that maternal plasma concentrations of unmethylated maspin sequences were found to be higher in 5.7fold in preeclamptic pregnancies that have dysfunction of trophoblast in-vasion and reduced neoangiogenesis of the placenta, compared to healthy pregnancy controls (21).…”

The value of maspin and PD-L1 expression and peritumoral lymphocytic infiltration in breast tumors

“…In this study; higher grade, lymph node metastasis and perineural invasion were found to be associated with cytoplasmic maspin expression. Therefore, cytoplasmic maspin expression is thought to be associated with poor prognosis (43). In a study of Gurzu et al, In colorectal carcinomas, tumors in which cytoplasmic maspin staining is dominant have a better prognosis and nuclear dominance is associated with high aggressiveness (44).…”