Masculinization protocol for Nile tilapia (O. niloticus) in Biofloc technology using 17-α-methyltestosterone in the diet

Silva, Rodrigo Zhouri Costa e; Alvarenga, Érika Ramos de; Matta, Sylvia Velloso; Alves, Gabriel Francisco de Oliveira; Manduca, Ludson Guimarães; Silva, Marcos Antonio; Yoshinaga, Thomás Toshio; Fernandes, Arthur Francisco Araújo; Turra, Eduardo Maldonado

doi:10.1016/j.aquaculture.2021.737470

Cited by 10 publications

(4 citation statements)

References 39 publications

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“…Other studies with masculinization using MT supplemented feed in different fish species, obtained a higher percentage of males as in the case of tilapia where it was possible to obtain 94% of males with 60 mg/kg (Silva et al, 2022) and for silver perch where 100% of males were obtained with 9 mg/kg (Sulaeman and Fotedar, 2017). In addition, a male percentage similar to ours was described for Rhamdia quelen with 72% males at a dose of 60 mg/kg (Weiss et al, 2018a).…”

Section: Discussionsupporting

confidence: 85%

Effect of dosage of orally administered 17α-methyltestosterone on sex reversion of the yellowtail tetra Astyanax lacustris (Lütken, 1875)

et al. 2023

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The females of yellowtail tetra (Astyanax lacustris), known as the freshwater sardine, are approximately 1.33 times larger than males, and thus, all-female monosex culture would increase production and reduce size variability. The present work aimed to identify the optimal dose of 17α-methyltestosterone (MT) to be used in the masculinization of A. lacustris for indirect sex reversal. Three different concentrations of MT (20, 40, and 60 mg/kg of feed in the diet) were fed to the fry for 30 days. Thirty adult individuals from each treatment, including the control (0 mg MT/kg), were evaluated for gonadal development, morphological and histological sexual identification, zootechnical performance, and the possible genotoxic effect caused by prolonged exposure to MT. MT significantly (P<0.01) affected the differentiation of the gonads, with the presence of possible inhibitory effects in all treatments. Intersex individuals were present in the 20 and 60 mg MT/kg treatments. All treatments were able to masculinize A. lacustris and the treatment with the lowest hormone concentration produced the highest percentage of males 76.7%, while the control had 46.7% males. The presence of erythrocyte nuclear alterations indicated a possible cytotoxic effect of MT in treatments 40 and 60 mg MT/kg, however, the use of the hormone did not affect the growth and the survival of the individuals. Thus, the use of MT is a viable option for obtaining neomales as a first step into the production of all-female progenies.

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Section: Discussionsupporting

confidence: 85%

Effect of dosage of orally administered 17α-methyltestosterone on sex reversion of the yellowtail tetra Astyanax lacustris (Lütken, 1875)

et al. 2023

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“…e Silva e al. (2022) [48] reported the 94% masculinization in tilapia after the fed of 17α-MT at 60 mg kg-1 five times daily on the BFT systems. When given 50 mg/kg of 17α-MT, an exogenous hormone, the juvenile yellow perch, Perca flavencens, generated up to 100% male offspring in contrast to the other treatment groups [33].…”

Section: Sex Ratio and Histologymentioning

confidence: 99%

Effects of dietary aromatase inhibitors on masculinization of rosy barb (Pethia conchonius): Evidence from growth, coloration and gonado-physiological changes

Lal,

Biswas,

Singh

et al. 2023

PLoS ONE

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The objective of this study was to reveal the growth, colouration and gonado-physiological changes due to the exogenous aromatase inhibitor (AIs) in an ornamental fish. 17α-methyltestosterone (MT) and letrozole (LET) were used as potential AIs. The AI were supplemented with a gel-based feed (LET: 50, 100, 150 and MT: 12.5, 25, 37.5 mg/kg feed) in Rosy barb, Pethia conchonius fry. The fishes were reared in a 45-L glass tank using AI treated gel-based feed for 3 months. Growth in AI-based diets was reduced but the reduction was minimal compared to the control. At 25 mg/kg feed of 17 MT, the highest male proportion (84.72% 6.05%) was recorded, which was significantly higher (P≤0.05) than other groups. L*, a*, and b* values showed that 17α-MT-fed groups had brighter coloration (P≤0.05). Histological sections showed that LET-17α-MT suppressed ovarian development, causing atretic oocytes. Testicular development was unaffected. 25 mg/kg-treated feed increased SOD, CAT, GST, and GPX. The AI (MT) at 25 mg/kg gel-based feed could therefore be utilised for musculinization without impacting growth, colour, and antioxidant activity of rosy barb, which serves the entire male population in the ornamental fish sector.

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“…In this way, fish do not move energy to reproduction, only to growth. Lots of monosex fish or sterile individuals can be obtained by sexing, sexual manipulations, using sexual steroids or chromosomal manipulations such as polyploidy ( Arai and Fujimoto, 2018 ; Baroiller and D’Cotta, 2018 ; Wang and Shen, 2018 ; Alvarenga et al, 2020 ; Costa e Silva et al, 2022 ). The effectiveness of sterility is also important for environmental sustainability because the escape of fertile fish represents a threat of genetic contamination of wild population stocks and possible ecological imbalance ( Wong and Zohar, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the most used method on sexual control in commercial tilapia production is the use of monosex populations through the sexual inversion induction by hormonal treatment ( Baroiller and D'Cotta, 2018 ; Costa e Silva et al, 2022 ). However, much is discussed about the sex steroids use in tilapia larvae, therefore, triploidy was considered as an alternative for reproductive control ( Teletchea and Fontaine, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Growth performance, reproductive status, and chromosomal instability in triploid Nile tilapias

Menezes,

Alvarenga,

Nóbrega

et al. 2024

Anim. Reprod.

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Reproductive control is one of the biggest challenges in tilapia production and triploidy was developed as an alternative to sterilization. In general, polyploids present chromosomal instability but for triploid Nile tilapia it has yet to be reported. This study evaluated the chromosomal instability from juveniles to adulthood, growth performance and gonadal status of tilapia hatched from eggs submitted or not to heat shock for triploid induction. Nile tilapia oocytes were fertilized (1,476 oocytes), half of the eggs were subjected to a four-minute shock in 41 °C water four minutes after fertilization and the other half were not (Control group). The eggs were incubated (at 27°C) and 160 larvae from the treated group hatched and survived after yolk sac absorption. The determination of ploidy was performed by flow cytometry at 85 th (juveniles) and 301 st (adults) days of age post yolk sac absorption. At the time of the first cytometry analysis there were 73 surviving juveniles from the treated group, and only 14 were confirmed triploid. However, at the analysis of adult ploidy, one out of 8 surviving adult tilapias from the 14 confirmed triploid juveniles remained triploid. Gonadal histology showed that the non-remaining triploids continued to produce gametes. The growth performance of triploid tilapia was initially superior to that of diploid tilapia during the juvenile phase, but similar in adults. Once the chromosome sets are lost and the tilapias become diploid again, at least in tissues with a high proliferation rate, such as the hematopoietic tissue that was analyzed (and possibly in gonads), all possible advantages of triploids are probably lost. Thus, our results suggest that, due to genomic instabilities, the triploid generation of tilapia has low efficiency.

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Masculinization protocol for Nile tilapia (O. niloticus) in Biofloc technology using 17-α-methyltestosterone in the diet

Cited by 10 publications

References 39 publications

Effect of dosage of orally administered 17α-methyltestosterone on sex reversion of the yellowtail tetra Astyanax lacustris (Lütken, 1875)

Effect of dosage of orally administered 17α-methyltestosterone on sex reversion of the yellowtail tetra Astyanax lacustris (Lütken, 1875)

Effects of dietary aromatase inhibitors on masculinization of rosy barb (Pethia conchonius): Evidence from growth, coloration and gonado-physiological changes

Growth performance, reproductive status, and chromosomal instability in triploid Nile tilapias

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