Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina

Abstract

This study aimed to examine the effect of extract of purwoceng Pimpinella alpina for masculinization of Betta splendens. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L as control. The treatments were given by immersion to 35 embryos at eye spots phase or about 28-hours after fertilization, for eight hours. The immersion process was done for 8 hours. The result showed that 20 µL/L dose of purwoceng extract produced 62.66% male, and hatching rate was 85.71%. This male percentage was higher compared to control (45.91%), but the hatching rate was lower than that of control (98.57%). At the higher dose (30 µL/L), male fish population and hatching rate reduced respectively 39.72% and 68.57%, respectively. Keywords: masculinization, embryo immersion, purwoceng extract, Betta splendens ABSTRAKPenelitian ini bertujuan untuk mengevaluasi pengaruh dosis ekstrak purwoceng Pimpinella alpina extract dalam maskulinisasi ikan cupang hias Betta splendens. Penelitian ini menggunakan metode rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan dosis ekstrak purwoceng. yaitu 10, 20, dan 30 µL/L, serta perlakuan 0 µL/L sebagai kontrol. Perlakuan diberikan melalui perendaman selama delapan jam terhadap 35 embrio saat memasuki fase bintik mata atau sekitar jam ke-28 setelah pembuahan. Hasil penelitian menunjukkan bahwa dosis ekstrak purwoceng 20 µL/L menghasilkan persentase ikan jantan sebesar 62,66%, dan daya tetas telur sebesar 85,71%. Persentase jantan tersebut lebih tinggi daripada kontrol (45,1%), tetapi derajat penetasannya lebih rendah daripada kontrol (98,57%). Pada dosis yang lebih tinggi (30 µL/L), populasi ikan jantan (39,72%), dan derajat penetasan (68,57%) adalah menurun.Kata kunci: maskulinisasi, perendaman embrio, ekstrak purwoceng, Betta splendens Artikel Orisinal

The Effects of Different Dosages of Pasak Bumi (Eurycoma longifolia Jack) towards The Sex of Betta Fish Embryo

“…The temperature and dissolved oxygen obtained during the study still support fish life where temperatures of 24-28 0C and DO > 3 mg/L are suitable in the maintenance of betta fish and sex direction in betta fish [5]; [27]. The pH of the water in the rearing container still supports the life of the fish that are kept, a pH ranging from 6-7 is a suitable pH in the maintenance of betta fish and the success of maleization in betta fish [6]; [28]; [29]; [27].…”

“…provide the aesthetic value of the fish [3]; [4]; [5]. Thus, there is a need for efforts to obtain a male monosex population using the masculinization method [6]; [1]; [5].…”

“…Natural ingredients such as coconut water can increase up to 86.26±8.34% male IOP Publishing doi:10.1088/1755-1315/1118/1/012005 2 sex ratio [5], honey can increase 93-96% [1]; 77, 33% [7] and 85-100% male sex ratio [3]. Other natural ingredients such as celery leaves can increase the sex ratio greater than 50% [8] and purwoceng extract can increase the sex ratio percentage up to 62.66% [6].…”

Maskulinisasi Ikan Platy Pedang (Xiphophorus hellerii) melalui Perendaman Larva dalam Larutan Madu dengan Dosis Berbeda

“…Menurut Arfah et al (2013), salah satu metode untuk meningkatkan produksi ikan jantan adalah melalui proses maskulinisasi. Menurut Siregar et al (2018), maskulinisasi merupakan teknik yang dapat digunakan untuk memproduksi benih ikan monosex jantan.…”

Evaluation of yellow pumpkin seed extract (Cucurbita moschata D) on the successful masculinization of betta fish (Betta splendens)

“…Persentase kelamin jantan pada setiap perlakuan mengalami kenaikan dan penurunan pada perlakuannya. Menurut Arfah et al (2013) hal ini menunjukkan semakin tinggi dosis perendaman dalam kegiatan jantanisasi ikan tidak selalu diikuti dengan peningkatan persentase populasi jantannya. Untuk menghasilkan perendaman yang efektif, perlu diperhatikan antara konsentrasi dosis yang diberikan dan lama perendamannya.…”