Marsdenia tenacissima extract disturbs the interaction between tumor-associated macrophages and non-small cell lung cancer cells by targeting HDGF

Fu, Jialei; Hao, Huifeng; Wang, Shan; Jiao, Yanna; Li, Pingping; Han, Shuyu

doi:10.1016/j.jep.2022.115607

Cited by 16 publications

(10 citation statements)

References 51 publications

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“…Additionally, they can also secrete immune stimulatory factors to promote the proliferation of NK cells as well as T cells to exert a stronger anti-tumor effect. M2 macrophages, which can (15,16). Previous studies have suggested that tumorinfiltrating macrophages are related to poor outcomes in lung cancer (17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

The relationship between tumor infiltrating immune cells and the prognosis of patients with lung adenocarcinoma

Tong¹,

Wang²,

Liu³

et al. 2023

J Thorac Dis

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Background: To depict the immune infiltration characteristics of tumor cells in patients with lung adenocarcinoma (LUAD) and evaluate the predictive value and significance of tumor immune cells on the prognosis of LUAD patients. Methods:The clinical characteristics and transcriptome of LUAD patients were obtained from The Cancer Genome Atlas (TCGA), and the immune cell abundance in LUAD tissue was evaluated using the CIBERSORT algorithm. We created a simplified immune cell-based Cox regression model according to the survival status of patients and clarified the correlation between the survival status of patients and seven types of immune cells. An immune cell-based risk prediction model was created by Cox proportional hazards regression. Subsequently, the gene expression profile of LUAD patients was obtained from the Gene Expression Omnibus (GEO) database to validate the tumor immune infiltration and patient prognosis prediction model attained using the CIBERSORT algorithm.Results: The abundance of 22 tumor-infiltrating immune cells in these patients was detected using the CIBERSORT algorithm. According to Pearson correlation analysis, the immune cells appeared to be closely related to each other. The immune cell composition was remarkably different between the LUAD tumor tissue and paracancerous tissue. The simplified COX model showed that seven kinds of immune cells have predictive value for the prognosis and survival status of LUAD. The receiver operating characteristic curve (ROC) curve confirmed that the prediction model performed well for 1-, 3-, and 5-year survival status. The calibration curve suggested that the prediction model was consistent with the clinical results. Correlation analysis revealed that the clinical features were significantly related to immune cell infiltration. A total of 246 LUAD specimens were from the GEO database, and the risk score model suggested that high risk scores were indicative of a poor prognosis. Finally, enzyme-linked immunosorbent assay (ELISA) revealed that the expressions of tumor necrosis factor-α (TNF-α), interleukin 8 (IL-8), IL-6, and interferon-γ (IFN-γ) in tumor tissues were remarkably higher compared with those in adjacent tissues.Conclusions: There is a close correlation between the tumor-infiltrating immune cells and the prognosis and clinical characteristics of LUAD patients. The risk score model based on TCGA and GEO designed in this study can be applied in clinical practice.

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Section: Discussionmentioning

confidence: 99%

The relationship between tumor infiltrating immune cells and the prognosis of patients with lung adenocarcinoma

Tong¹,

Wang²,

Liu³

et al. 2023

J Thorac Dis

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“…JAK1 is the upstream signal of STAT3 (Fu et al, 2022). The binding of extracellular signal to STAT3 can promote STAT3 phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

“…From our results, HIPK2 overexpression promoted M1 polarization of BV2 cells, increased p‐STAT3 expression, but did not significantly affect JAK1 expression. JAK1 is the upstream signal of STAT3 (Fu et al, 2022). The binding of extracellular signal to STAT3 can promote STAT3 phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

“…Research discovers that characteristic changes of neuroinflammation exist in depressive patients, which mostly manifest as the activation and M1 polarization of microglial cells in related brain areas (Larosa & Wong, 2022). During microglial cell polarization, STAT3 phosphorylation is a key process (Fu et al, 2022), but the regulation on STAT3 phosphorylation remains unclear. In this study, it was discovered based on GEO database that HIPK2 was significantly expressed in depression, which was positively related to STAT3.…”

Section: Introductionmentioning

confidence: 99%

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HIPK2 mediates M1 polarization of microglial cells via STAT3: A new mechanism of depression‐related neuroinflammation

Han

Pei

Sheng

et al. 2023

Journal Cellular Physiology

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This study aimed to investigate the role of protein kinase HIPK2 in depression and its associated mechanism. The chronic unpredictable mild stress (CUSM) model was constructed to simulate mice with depression to detect the mouse behaviors. Moreover, by using mouse microglial cells BV2 as the model. After conditional knockdown of HIPK2, the depressive behavior disorder of mice was improved, meanwhile, neuroinflammation was alleviated, and the M1 cell proportion was reduced. Similar results were obtained after applying the HIPK2 inhibitor tBID or ASO‐HIPK2 treatment. HIPK2 was overexpressed in BV2 cells, which promoted M1 polarization of cells, while tBID suppressed the effect of HIPK2 and reduced the M1 polarized level in BV2 cells. Pull‐down assay results indicated that HIPK2 bound to STAT3 and promoted STAT3 phosphorylation. We found that HIPK2 can bind to STAT3 to promote its phosphorylation, which accelerates M1 polarization of microglial cells, aggravates the depressive neuroinflammation, and leads to abnormal behaviors. HIPK2 is promising as the new therapeutic target of depression.

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“…Xie et al, 2022). In addition, previous studies suggested that STAT3 could play a crucial role in TAMs as its upregulation could promote macrophage M2 polarization (J. L. Fu et al, 2022;Yang et al, 2022). However, the detailed relation between IGF2BP3 and STAT3 in tumor microenvironment during GBC progression remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of IGF2BP3 inhibits the tumorigenesis of gallbladder cancer and modifies tumor microenvironment

Qin

Zhang

Lei

et al. 2022

Drug Development Research

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Gallbladder cancer (GBC) ranks seventh among the gastrointestinal cancers. Messenger RNAs (mRNAs) could regulate the progression of GBC. For the purpose of exploring the targets for GBC treatment, RNA sequencing was used to identify the differential expressed mRNAs between GBC and adjacent tissues. Next, CCK8 assay was used to assess the cell viability, and cell proliferation was investigated by colony formation assay. Flow cytometry was performed to evaluate the cell apoptosis. Protein and mRNA expression were analyzed by western blot and RT‐qPCR, respectively. Transwell was performed to evaluate the cell metastasis. GBC‐derived exosomes were isolated with ultracentrifugation. To evaluate the function of exosomes in GBC, in vivo model of GBC was constructed. The data revealed IGF2BP3 was identified to be upregulated in GBC, and IGF2BP3 silencing was able to decrease GBC cell proliferation by promoting the apoptosis. The migration and invasion of GBC cells were reduced by IGF2BP3 knockdown. Silencing of IGF2BP3 obviously suppressed the level of p‐STAT3 in GBC cells. Meanwhile, GBC cell‐derived exosomes notably promoted macrophage M2 polarization via carrying IGF2BP3, and then the polarized macrophages promoted the malignant behavior of GBC cells. Furthermore, exosomes markedly promoted the tumor growth of GBC via promoting macrophage M2 polarization. In summary, knockdown of IGF2BP3 suppressed the malignant behavior of GBC cells. Additionally, knockdown of IGF2BP3 modified tumor microenvironment during the progression of GBC. Thus, these findings might provide a new theoretical basis for exploring a strategies against GBC.

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Marsdenia tenacissima extract disturbs the interaction between tumor-associated macrophages and non-small cell lung cancer cells by targeting HDGF

Cited by 16 publications

References 51 publications

The relationship between tumor infiltrating immune cells and the prognosis of patients with lung adenocarcinoma

The relationship between tumor infiltrating immune cells and the prognosis of patients with lung adenocarcinoma

HIPK2 mediates M1 polarization of microglial cells via STAT3: A new mechanism of depression‐related neuroinflammation

Knockdown of IGF2BP3 inhibits the tumorigenesis of gallbladder cancer and modifies tumor microenvironment

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