Marrow culture studies in adult acute myeloid leukemia at diagnosis and during complete remission

Shih, Lee‐Yung; Chang, Chyuan-Ying

doi:10.1007/bf00320299

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…It is difficult to compare our findings with those of others due to differences in methodology and criteria for classifying growth pat terns. Our previous work had shown that there was no correlation between culture growth pattern and age or initial WBC count [8]. The same analyses were carried out in respect to the Hb level, platelet count and per centage of leukemic cells in marrow.…”

Section: Discussionmentioning

confidence: 98%

“…Studies reported previously have shown that mar row cultures from patients with untreated acute non lymphocytic leukemia (ANLL) exhibited heterogene ous abnormal growth patterns [1][2][3][4][5][6][7][8]. However, at tempts to correlate the growth pattern with the out come of therapy in ANLL have yielded conflicting re sults by several groups [ 1 -7,9-19].…”

Section: Introductionmentioning

confidence: 99%

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Prognostic Significance of in vitro Marrow Culture Growth Pattern in Untreated Acute Nonlymphocytic Leukemia

Shih¹,

Dunn²,

Liaw³

1986

Acta Haematol

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The growth pattern of marrow cells in agar culture was studied in 90 adult patients with acute nonlymphocytic leukemia (ANLL) at diagnosis. We classified the abnormal growth patterns into 4 groups, A: no growth, B: decreased growth, C: excessive microcluster formation and D: excessive cluster growth with more than 20 colonies. There was a good correlation between growth pattern and FAB subtype. A predominance of group A growth was observed in Ml, while group B growth was found in 50% of patients with M2 and M5. No relationships between the growth patterns and other clinical parameters were detected. Sixty-six patients were evaluable for treatment outcome. The growth pattern significantly correlated with complete remission rate. The remission rates were 52, 87, 80, and 25% for patients with group A, B, C and D growth, respectively. Analyses of remission duration and survival curves showed significant differences among the different growth patterns. Patients with D growth experienced a shorter remission duration and a lower survival rate than other groups. These results indicate that the in vitro culture growth pattern in untreated ANLL is of prognostic significance in predicting the response to therapy.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Prognostic Significance of in vitro Marrow Culture Growth Pattern in Untreated Acute Nonlymphocytic Leukemia

Shih¹,

Dunn²,

Liaw³

1986

Acta Haematol

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“…In vitro culture has been increasingly used to study the proliferative characteristics of human leukemias, especially acute myeloid leukemia [1, 2, 3, 4, 5, 6, 7]. Marrow cultures exhibited abnormal growth patterns ranging from no growth to excessive microcluster formation in acute myeloid leukemia.…”

Section: Introductionmentioning

confidence: 99%

Granulocyte-Macrophage Colony-Forming-Unit Assay and Liquid Suspension Culture of Bone Marrow and Peripheral Blood in Newly Diagnosed Childhood Acute Lymphoblastic Leukemia

et al. 1998

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Granulocyte-macrophage colony-forming-unit (CFU-GM)-assays and/or liquid suspension cultures of bone marrow (BM) and peripheral blood (PB), using human placental conditioned medium as a source of colony-stimulating activity, were performed in 125 children with newly diagnosed acute lymphoblastic leukemia (ALL). Unlike the previous small series which invariably showed poor BM granulocyte-macrophage colony and cluster formations, we found 3 growth patterns. A poor-growth pattern was seen in 101 patients. A low-growth pattern with BM colony and cluster formations in the lower range of the normal limits was observed in 6 patients. All 6 patients had low-risk ALL, and the in vitro growth may reflect the residual normal CFU-GM in BM. Thirdly an over-growth pattern was noted in one patient with refractory ALL. In 49 cases where liquid suspension cultures of BM were performed, 4 had a normal-growth pattern and 42 had apoptotic cells and/or few residual granulocytic/monocytic cells after 7 days of culture. However, in both B cell ALL cases and in 1 refractory T cell ALL case with hyperleukocytosis, repetitive liquid suspension cultures of BM and PB consistently revealed an extensive growth of lymphoblasts. Thus, an over-growth pattern in either the BM CFU-GM assay or liquid suspension culture of BM or PB in children with ALL at diagnosis may indicate a refractory or aggressive disease. A low-growth pattern in the BM CFU-GM assay in children with ALL at diagnosis probably predicts a favorable outcome.

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In vitro culture studies of blood and marrow cells in chronic myeloid leukemia at different phases of the disease

Shih

Chiu

Dunn

et al. 1988

Blut

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The in vitro culture growth of peripheral blood (PB) and bone marrow (BM) cells were studied simultaneously from 100 adult patients with chronic myeloid leukemia at different phases. Sixty-five patients were investigated at initial diagnosis, 30 patients in control phase, and 41 patients in blast phase. In untreated chronic phase, the relative concentrations of granulocyte-macrophage progenitor cells (CFU-GM) in BM were not significantly different from those of normal controls, but there was generally a marked increase in circulating CFU-GM. The 6 Ph1-negative patients did not show different growth characteristics. We were unable to correlate the CFU-GM number to any of the hematologic parameters as well as to the response to busulfan therapy. Pretreated patients with excessive cluster formation did not necessarily indicate impending blast crisis. In hematologic remission, the numbers of CFU-GM in both BM and PB were well within the ranges of normal controls. Culture results in blast phase revealed a spectrum of abnormal growth. In myeloid crisis, 14/29 BM and 12/29 PB samples showed increased colony and cluster formations which were composed predominantly of immature cells with variable degeneration. Marrow cells in lymphoid crisis produced low numbers of both colonies and clusters in 5 out of 8 patients, while blood cells from 8 out of 10 patients formed large amount of colonies of normal morphology. This study indicates that the in vitro CFU-GM assay may have diagnostic utility in differentiating lymphoid crisis from myeloid crisis.

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Marrow culture studies in adult acute myeloid leukemia at diagnosis and during complete remission

Cited by 8 publications

References 28 publications

Prognostic Significance of in vitro Marrow Culture Growth Pattern in Untreated Acute Nonlymphocytic Leukemia

Prognostic Significance of in vitro Marrow Culture Growth Pattern in Untreated Acute Nonlymphocytic Leukemia

Granulocyte-Macrophage Colony-Forming-Unit Assay and Liquid Suspension Culture of Bone Marrow and Peripheral Blood in Newly Diagnosed Childhood Acute Lymphoblastic Leukemia

In vitro culture studies of blood and marrow cells in chronic myeloid leukemia at different phases of the disease

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