Marrow adipose tissue accumulation and dysgenesis of the trabecular bone after anterior cruciate ligament transection and reconstruction in the rat proximal tibial epiphysis

Kaneguchi, Akinori; Ozawa, Junya; Umehara, Takuya; Yamaoka, Kaoru

doi:10.1016/j.acthis.2022.151891

Cited by 3 publications

(9 citation statements)

References 41 publications

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“…In this study, trabecular bone and MAT areas in the ACLR group were comparable to those of the control group, although a previous study reported MAT accumulation in the rat proximal tibial epiphysis at 4 and 12 weeks after ACL reconstruction [ 16 ]. Another study reported that trabecular bone and MAT areas in the proximal tibial epiphysis did not change significantly after two and four weeks of ACL reconstruction in rats [ 18 ].…”

Section: Discussionsupporting

confidence: 50%

“…Sagittal sections (4 μm thick) were cut at the medial midcondylar level using a microtome and stained with Safranin-O Fast Green. Parameters of trabecular bone and MAT in the proximal tibial epiphysis were assessed using previously described methods [ 16 , 18 ]. Six fields of view located between the articular cartilage and the growth plate were photographed at 20× magnification using a light microscope (BX51; Olympus, Tokyo, Japan) equipped with a digital microscope camera (WRAYCAM-NOA2000; WRAYMER, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…ACL injury is the most common knee injury and can result in bone loss around the knee in human patients [ 25 ]. In a rat ACL transection model, bone loss in the proximal tibial epiphysis was accompanied by MAT accumulation [ 16 ]. The most common treatment for ACL injury is ligament reconstruction surgery.…”

Section: Introductionmentioning

confidence: 99%

“…The most common treatment for ACL injury is ligament reconstruction surgery. A previous study reported that immediate ligament reconstruction after ACL transection inhibited trabecular bone loss but could not block MAT accumulation in the proximal tibial epiphysis in rats [ 16 ]. Another study reported decreased bone mineral density in the proximal tibia after ACL reconstruction in human patients [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Weight Bearing on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in the Rat Proximal Tibial Epiphysis

Kaneguchi,

Yamaoka,

Ozawa

2024

Acta Histochem. Cytochem

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The effects of mechanical unloading after anterior cruciate ligament (ACL) reconstruction on bone and marrow adipose tissue (MAT) are unclear. We investigated weight bearing effects on bone and MAT after ACL reconstruction. Rats underwent unilateral knee ACL transection and reconstruction, followed by hindlimb unloading (non-weight bearing), no intervention (low-weight bearing, the hindlimb standing time ratio (STR; operated/contralateral) during treadmill locomotion ranging from 0.55 to 0.91), or sustained morphine administration (moderate-weight bearing, STR ranging from 0.80 to 0.95). Untreated rats were used as controls. At 7 or 14 days after surgery, changes in trabecular bone and MAT in the proximal tibial were assessed histologically. Histological assessments at 7 or 14 days after surgery showed that ACL reconstruction without post-operative intervention did not significantly change trabecular bone and MAT areas. Hindlimb unloading after ACL reconstruction induced MAT accumulation with adipocyte hyperplasia and hypertrophy within 14 days, but did not significantly affect trabecular bone area. Increased weight bearing through morphine administration did not affect trabecular bone and MAT parameters. Our results suggest that early weight bearing after ACL reconstruction is important in reducing MAT accumulation, and that reduction in weight bearing alone is not sufficient to induce bone loss early after ACL reconstruction.

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Section: Discussionsupporting

confidence: 50%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Weight Bearing on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in the Rat Proximal Tibial Epiphysis

Kaneguchi,

Yamaoka,

Ozawa

2024

Acta Histochem. Cytochem

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“…ACL reconstruction can cause marrow adipose tissue (MAT) accumulation and bone loss in the bones around knee joint [ 13 , 21 ]. For example, MAT accumulation in the proximal tibia has been observed in rats after ACL reconstruction [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Corticosteroid Administration and Treadmill Exercise on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in Rats

Kaneguchi,

Yamaoka,

Ozawa

2024

Acta Histochem. Cytochem

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We aimed to investigate the effects of short-term corticosteroid administration after anterior cruciate ligament (ACL) reconstruction on marrow adipose tissue (MAT) and trabecular bone mass, as well as to examine whether treadmill exercise can mitigate MAT increase and trabecular bone deterioration caused by corticosteroid. ACL-reconstructed rats were divided into groups: no intervention, daily treadmill exercise (60 min/day), administration of the steroidal drug dexamethasone (250 μg/kg on days 0–5, 7, and 9 post-operatively), or dexamethasone administration combined with treadmill exercise. Untreated rats were served as controls. At day 10 or 30 post-operatively, histological assessments were performed in the proximal tibial epiphysis. MAT accumulation and trabecular bone loss were observed after ACL reconstruction. Dexamethasone promoted MAT accumulation at day 10 post-operatively but did not affect the trabecular bone loss. The MAT accumulation caused by dexamethasone reversed within 21 days after discontinuation. Treadmill exercise did not influence the changes in the MAT and trabecular bone areas. Short-term corticosteroid administration after ACL reconstruction promoted MAT accumulation while not affecting trabecular bone area. The MAT accumulation resulting from corticosteroid administration was reversible after discontinuation. Treadmill exercise could not mitigate the accumulation of MAT caused by corticosteroid administration and did not affect trabecular bone area.

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Effects of joint immobilization and treadmill exercise on marrow adipose tissue and trabecular bone after anterior cruciate ligament reconstruction in the rat proximal tibial epiphysis

2023

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Marrow adipose tissue accumulation and dysgenesis of the trabecular bone after anterior cruciate ligament transection and reconstruction in the rat proximal tibial epiphysis

Cited by 3 publications

References 41 publications

Effects of Weight Bearing on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in the Rat Proximal Tibial Epiphysis

Effects of Weight Bearing on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in the Rat Proximal Tibial Epiphysis

The Effects of Corticosteroid Administration and Treadmill Exercise on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in Rats

Effects of joint immobilization and treadmill exercise on marrow adipose tissue and trabecular bone after anterior cruciate ligament reconstruction in the rat proximal tibial epiphysis

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