Marmoset glutathione peroxidases: cDNA sequences, molecular evolution, and gene expression

Atanasova, Srebrena; Ahsen, Nicolas von; Schlumbohm, Christina; Wieland, Eberhard; Oellerich, Michael; Armstrong, Victor W.

doi:10.1111/j.1600-0684.2006.00158.x

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…In addition, the accumulation of oxide reactive species rep-rate and the morbidity and mortality associated with bladder cancer vary according to ethnic group, gender, resents an important role in the pathogenesis of cardiovascular diseases, neurodegenerative disorders, chronic and age (2,3). In Ecuador, bladder cancer represents the 19th and 20th cause of death in men and in women, damage to the liver and kidneys, as well as the development of different types of cancer (8). respectively (4,5).…”

Section: Introductionmentioning

confidence: 99%

Frequency of Polymorphisms pro198leu in GPX-1 Gene and ile58thr in MnSOD Gene in the Altitude Ecuadorian Population With Bladder Cancer

Paz‐y‐Miño¹,

Muñoz²,

López‐Cortés³

et al. 2009

Oncology Research Featuring Preclinical and Clinical Cancer The

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Bladder cancer represents 8% of all malignancies diagnosed in men and 3% in women. The risk factors for developing bladder cancer, including the incidence rate, morbidity, and mortality, vary according to the ethnic group, exposition rate at work, age, gender, and tobacco consumption. Moreover, there is a risk of developing this carcinoma due to dietary conditions, demonstrating that certain enzymes neutralize oxidative compound derivative of carcinogens, which if not degraded, accumulate in the body and destroy epithelial cells of the bladder, causing an increase in the risk of developing this disease. The detoxifying enzymes inactivate dangerous chemical compounds and anions for the cell; that is, why it is important to know if the polymorphisms pro198leu in GPX-1 and ile58thr in MnSOD are associated with bladder cancer. In this study, 120 individuals were analyzed as controls and 97 individuals with previously diagnosed bladder cancer. In the case of polymorphism pro198leu, highly significant differences were observed and individuals with this polymorphism presented a probability of developing bladder cancer 3.8 times greater than controls (OR = 3.8; 95% CI 2.16-6.78; p < 0.001). No significant differences in polymorphism ile58thr of MnSOD gene occurred when we compared the study population (OR = 2.1; 95% CI 1.26-3.49; p > 0.05). The results indicate that polymorphism of GPX-1 gene influences the risk of developing bladder cancer in the Ecuadorian population, suggesting that more research on detoxifying genes in bladder cancer should be conducted.

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Section: Introductionmentioning

confidence: 99%

Frequency of Polymorphisms pro198leu in GPX-1 Gene and ile58thr in MnSOD Gene in the Altitude Ecuadorian Population With Bladder Cancer

Paz‐y‐Miño¹,

Muñoz²,

López‐Cortés³

et al. 2009

Oncology Research Featuring Preclinical and Clinical Cancer The

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Prenatal dexamethasone exposure in the common marmoset monkey enhances gene expression of antioxidant enzymes in the aorta of adult offspring

Atanasova

Wieland

Schlumbohm

et al. 2009

Stress

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Human epidemiological studies have indicated that low birth weight associated with an adverse intrauterine environment is related to a greater incidence of cardiovascular disorders in later life. In the foetus, endogenous glucocorticoids generally increase if there is intrauterine nutrient deficiency. The consequent glucocorticoid hyperexposure has been hypothesised to cause in utero programming of atherogenic genes. We investigated the effect of oral treatment with the synthetic glucocorticoid dexamethasone during early or late pregnancy in marmoset monkeys on oxidative and antioxidant status in the offspring. Urinary concentrations of F(2)-isoprostanes were quantified as markers for in vivo oxidative stress. Expression of the mRNAs for the antioxidant enzymes cytosolic glutathione peroxidase (GPx-1), phospholipid hydroperoxide glutathione peroxidase (GPx-4), cytosolic Cu,Zn-superoxide dismutase (SOD1), mitochondrial Mn-superoxide dismutase (SOD2), glutathione reductase (GSR), modifier subunit of glutamate-cysteine ligase (GCLM) and catalase were determined in the aorta. Three groups of pregnant marmosets (10 animals per group) were treated orally for one week with vehicle, or with dexamethasone (5 mg/kg daily) during two gestation windows: early dexamethasone group, pregnancy day 42-48, and late dexamethasone group, pregnancy day 90-96. In one male sibling of each litter (10 males per group), aortas were taken at 2 years of age. In the late dexamethasone group a higher aortic mRNA expression for GPx-1 (p < 0.023), MnSOD (p < 0.016), GCLM (p < 0.019) and GSR (p < 0.014) in comparison to the controls was observed. Aortic expression in the early dexamethasone group was statistically significantly higher only for GSR mRNA (p < 0.038). No significant changes in urinary F(2)-isoprostane concentrations between controls, early and late dexamethasone groups at 2 years of age were observed. Hence, prenatal exposure to dexamethasone in the third trimester leads to increased mRNA expression of several aortic antioxidant enzymes in the offspring. This expression pattern was not temporally related to oxidative stress, and it may reflect in utero re-programming of aortic antioxidant gene expression during prenatal glucocorticoid exposure.

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Selenium in mammalian spermiogenesis

Flohé¹

2007

BCHM

147

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The role of selenium in male fertility is reviewed with special emphasis on selenoprotein P and phospholipid hydroperoxide glutathione peroxidase (GPx4) in spermiogenesis. Inverse genetics reveal that selenoprotein P is required for selenium supply to the testis. GPx4 is abundantly synthesized in spermatids. As a moonlighting protein it is transformed in the later stages of spermiogenesis from an active selenoperoxidase into a structural protein that becomes a constituent of the mitochondrial sheath of spermatozoa. The transformation is paralleled by loss of glutathione. Mechanistically, the process is an alternate substrate inactivation of GPx4 resulting from reactions of its selenenic form with thiols of GPx4 itself and other proteins. Circumstantial evidence and ongoing experimental genetics indicate that the mitochondrially expressed form of the GPx4 gene is the most relevant one in spermiogenesis, with the nuclear form being dispensable for fertility and the role of cytosolic GPx4 remaining unclear. Clinical data reveal a strong association of low sperm GPx4 with infertility. Thus, impaired GPx4 biosynthesis, due to selenium deficiency or to genetic defects in gpx4 itself or in proteins involved in Se distribution and selenoprotein biosynthesis, causes male infertility, but can also be an epiphenomenon due to any perturbation of testicular function.

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Marmoset glutathione peroxidases: cDNA sequences, molecular evolution, and gene expression

Abstract: These findings will be of value for studies designed to assess the role of glutathione peroxidases in non-human primate models for a variety of diseases in which increased oxidative stress has been implicated.

Cited by 3 publications

References 31 publications

Frequency of Polymorphisms pro198leu in <I>GPX-1</I> Gene and ile58thr in <I>MnSOD</I> Gene in the Altitude Ecuadorian Population With Bladder Cancer

Frequency of Polymorphisms pro198leu in <I>GPX-1</I> Gene and ile58thr in <I>MnSOD</I> Gene in the Altitude Ecuadorian Population With Bladder Cancer

Prenatal dexamethasone exposure in the common marmoset monkey enhances gene expression of antioxidant enzymes in the aorta of adult offspring

Selenium in mammalian spermiogenesis

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