Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization

Bednarska-Czerwińska, Anna; Czerwiński, Michał; Morawiec, Emilia; Łach, Aleksandra; Ziaja, Anna; Kusaj, Adrian; Strączyńska, Patrycja; Sagan, Dorota; Boroń, Dariusz; Grabarek, Beniamin Oskar

doi:10.3390/jcm11123348

Cited by 8 publications

(5 citation statements)

References 35 publications

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“…The predominance of Lactobacillus and the negative impact of Escherichia coli and Gardnerella vaginalis on fertility outcomes in their study reinforce the importance of a balanced endometrial microbiota for successful implantation. Additionally, another previous study identified both physiological and pathological microflora in the endometrium and cervix of women undergoing IVF, underscoring the potential influence of these microorganisms on reproductive success [20].…”

Section: Discussionmentioning

confidence: 72%

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Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure

Zhang,

Zou,

Zhang

et al. 2024

Ann Clin Microbiol Antimicrob

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Background Chronic endometritis (CE) is associated with poor reproductive outcomes, yet the role of endometrial microbiota in patients with recurrent implantation failure (RIF) and CE remains unclear. This study aims to characterize endometrial microbiota in RIF patients with CE and assess its implications for reproductive outcomes. Methods In this prospective study, we enrolled RIF patients both with and without CE. Endometrial and cervical samples were collected for 16 S rRNA gene sequencing. Microbiota composition was compared between groups using diversity indices, phylum, and genus-level analysis. Canonical correlation analysis (CCA) and Spearman’s correlation coefficients were used to assess relationships between CE, reproductive outcomes, and microbiota. Predictive functional profiling was performed to evaluate metabolic pathways associated with CE. Results Endometrial microbiota in CE patients exhibited greater diversity and evenness compared to non-CE patients. Principal coordinates analysis (PCoA) revealed distinct clustering between CE and non-CE groups. Linear discriminant analysis (LDA) identified Proteobacteria, Aminicenantales, and Chloroflexaceae as characteristic of CE, while Lactobacillus, Acinetobacter, Herbaspirillum, Ralstonia, Shewanela, and Micrococcaceae were associated with non-CE. CCA demonstrated associations between CE, adverse reproductive outcomes, and specific bacterial taxa. Microbial metabolic pathways significantly differed between CE and non-CE groups, with enrichment in pathways related to cofactors, vitamins, secondary metabolites, and the immune system in CE patients. Conclusion RIF patients with CE exhibit distinct endometrial microbiota compositions associated with adverse reproductive outcomes. The increased microbial diversity and altered metabolic pathways in CE suggest a potential correlation with reproductive outcomes, although further studies are necessary to elucidate the causal relationship between microbiota alterations and fertility. Modulating the endometrial microbiome may represent a novel therapeutic strategy to improve IVF outcomes in patients with CE.

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Section: Discussionmentioning

confidence: 72%

“…Miscarriage was defined as the loss of a pregnancy before the completion of 12 weeks of gestation. The inclusion and exclusion criteria were modified from a previous study (Supplemental Table S1) [20].…”

Section: Study Participantsmentioning

confidence: 99%

Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure

Zhang,

Zou,

Zhang

et al. 2024

Ann Clin Microbiol Antimicrob

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“…New technologies have enhanced the metagenomic analysis of samples that allow us to further characterise the microbiome of the reproductive tract [ 33 ]. We have better characterised the normal and abnormal endometrial microbiome and determined that sites previously thought to be sterile actually have robust microbiomes.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Endometrial Microbiome in Patients with Recurrent Implantation Failure

et al. 2023

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An abnormal endometrial microbiota has been associated with implantation failure; therefore, it may be important to evaluate it in order to improve reproductive outcomes in infertile patients. The main objective of our study was to compare the endometrial microbiome of patients with recurrent implantation failure (RIF) and control patients undergoing assisted reproduction treatment (ART). A prospective cohort study including forty-five patients with their own or donated gametes. The endometrial microbiome was analysed by massive sequencing of the bacterial 16S rRNA gene. Different bacterial communities were detected in RIF and control patients. Lactobacillus stands out as the most frequent genus, with 92.27% in RIF patients and 97.96% in control patients, and significant differences were reported between the two groups (p = 0.002). No significant differences were found regarding alpha diversity index. In beta diversity analysis, a significant trend was observed in the separation of the bacterial community between established groups (p < 0.07). Relative abundance analysis identified genera Prevotella (p < 0.001), Streptococcus (p < 0.001), Bifidobacterium (p = 0.002), Lactobacillus (p = 0.002) and Dialister (p = 0.003). Our results demonstrated the existence of an endometrial microbiota characteristic of RIF patients and showed that there might be a relationship between population of the endometrial microbiome and embryo implantation failure, providing us the possibility to improve clinical results in this patients.

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“…Table 1 presents the inclusion and exclusion criteria, which are the same as in our previous publication [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Dynamics of Microbiome Changes in the Endometrium and Uterine Cervix during Embryo Implantation: A Comparative Analysis

Bednarska-Czerwińska,

Morawiec,

Zmarzły

et al. 2023

Med Sci Monit

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Background The microbiome is the collection of all micro-organisms and their genes, which naturally live in and on the body. The cervical and endometrial bacterial microbiome has previously been reported to affect fertility and influence the outcomes of assisted reproductive therapy (ART), including embryo transfer. This study aimed to evaluate the cervical and endometrial bacterial microbiome in 177 women treated for infertility before, during, and after embryo implantation, and the outcomes. Material/Methods Cervical and endometrial swabs were collected from 177 women diagnosed with infertility at 3 time points: (1) during the initial examination, (2) during implantation, (3) 10–14 days after implantation. Next-generation sequencing (NGS) was used to analyze the bacterial microbiome. Taxonomic identification was performed with the Usearch algorithm. Results There was a significant change in the number of patients with Escherichia coli depending on the collection time. For the first swab collection, there were significant negative relationships between the percentage of Gardnerella vaginalis and Lactobacillus spp. For the second collection, there was a negative relationship between Lactobacillus helveticus and Lactobacillus jensenii . For the third collection, negative relationships were found between Escherichia coli and Lactobacillus spp. A similar distribution of the bacterial microbiome was observed in all 3 swab collections. Conclusions Lactobacillus spp. were the main bacteria identified in the cervix and endometrium, present before, during, and after successful embryo transfer. E. coli and G. vaginalis reduced the protective effect of Lactobacilli before, during, and after embryo transfer.

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Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization

Cited by 8 publications

References 35 publications

Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure

Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure

Characterization of the Endometrial Microbiome in Patients with Recurrent Implantation Failure

Dynamics of Microbiome Changes in the Endometrium and Uterine Cervix during Embryo Implantation: A Comparative Analysis

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