Markers of genotoxicity and oxidative stress in farmers exposed to pesticides

Jacobsen-Pereira, Carolina Hilgert; Santos, Cláudia Regina dos; Maraslis, Flora Troina; Pimentel, Luisi; Feijó, Ana Júlia Lobo; Silva, Clarice Iomara; Medeiros, Guilherme da Silva de; Zeferino, Rodrigo C.; Pedrosa, Rozangela Curi; Maluf, Sharbel Weidner

doi:10.1016/j.ecoenv.2017.10.004

Cited by 75 publications

(47 citation statements)

References 52 publications

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“…The reduction in PChE activity was associated with a higher level of PrC, which was the biomarker of pesticide exposure-induced oxidative stress damage. In contrast to previous studies [ 44 , 48 , 66 ], the present study showed that the decrease in PChE activity was associated with a lower level of TBARS. The significant relationship between oxidative stress and genotoxicity biomarkers, as shown in the results reported by Zepeda-Arce et al [ 55 ] and Jacobsen-Pereira et al [ 66 ], shows that pesticide exposure could lead to DNA damage and apoptosis by inducing oxidative stress via the generation of ROS and alternation of the capacity of the antioxidant system.…”

Section: Discussioncontrasting

confidence: 99%

“…The Spearman’s correlation analysis showed significant correlations between all oxidative stress biomarkers with PChE activity as a biomarker of exposure to anticholinesterase pesticides in the exposed group. In accordance with a previous study [ 66 ], an inverse association between PChE activity and DNA damage indices was found in the present study. In fact, some authors consider that cholinesterase inhibition induces cholinergic hyperactivity, initiating the accumulation of free radicals and leading to the damage of cellular components and pesticide-induced toxicity [ 68 , 69 ].…”

Section: Discussionsupporting

confidence: 94%

“…The cleavage of chromatin DNA into inter-nucleosomal fragments has a key role in the agrochemical-induced genotoxicity. The results of the Comet assay presented in this study, together with earlier studies [ 38 , 53 , 54 , 63 , 64 , 65 , 66 ], revealed that the occupational exposure to pesticides could induce DNA damage. However, Zepeda-Arce et al [ 55 ] reported no statistical differences in the measured genotoxic parameters among the study groups.…”

Section: Discussionsupporting

confidence: 79%

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Hematological Abnormality, Oxidative Stress, and Genotoxicity Induction in the Greenhouse Pesticide Sprayers; Investigating the Role of NQO1 Gene Polymorphism

Ahmadi

Mandegary

Jamshidzadeh

et al. 2018

Toxics

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The widespread use of pesticides in agriculture represents a threat to the human populations exposed to them. In this cross-sectional study, the hematological and biochemical parameters, plasma cholinesterase (PChE) activity, oxidative stress, genotoxicity, and NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism were measured in 100 greenhouse workers occupationally exposed to pesticide mixture and 104 normal healthy controls. There was a decrease in erythrocytes (5.45%, p = 0.026) and hemoglobin (3.26%, p = 0.025), and an increase in mean corpuscular hemoglobin (3.54%, p = 0.013) in the exposed workers. Sprayers showed a reduction in PChE (23%) and GSH (50%) levels, and an increase in lipid peroxidation (LPO) (55%), protein carbonyl (145%), Superoxide dismutase activity (61%), and total antioxidant capacity (35%) (p < 0.001 for all parameters but LPO: p = 0.009). Genotoxicity parameters were significantly high in the exposed cases (for all parameters: p < 0.001 but tail length: p = 0.002). There was a significant correlation between oxidative stress and genotoxicity parameters, and also between these biomarkers and PChE activity. The NQO1 C609T polymorphism was not significantly associated with studied biomarkers. The findings indicate that occupational exposure to a mixture of pesticides can induce hematotoxicity, oxidative stress, and genotoxicity in greenhouse workers.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 79%

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Hematological Abnormality, Oxidative Stress, and Genotoxicity Induction in the Greenhouse Pesticide Sprayers; Investigating the Role of NQO1 Gene Polymorphism

Ahmadi

Mandegary

Jamshidzadeh

et al. 2018

Toxics

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“…Regarding the DNA fragmentation in OP poisoning cases, it significantly increased (p < 0.001). This could be attributed to DNA damage and apoptosis by the induction of the oxidative stress resulting from the pesticide [42]. D'Costa et al are in accordance with the present study that observed significant DNA damage in all the fish treated with OP compounds [43].…”

Section: Controlsupporting

confidence: 92%

Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning

Tallat

Hussien

Mohamed

et al. 2020

Journal of Genetic Engineering and Biotechnology

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Background: Organophosphorus (OP) compounds have been widely available for decades in agriculture for crop protection and as cheap pest controllers, which increases the rate of exposure and poisoning cases. Using serum cholinesterase as prognostic markers for the acute OP toxicity is controversial; therefore, we aim to find out prognostic biomarkers that best correlate with mortality and outcomes of patients with acute OP toxicity. Levels of serum oxidative stress biomarkers (malondialdehyde (MDA) and total antioxidant capacity (TAC)) and activity of the apoptotic biomarkers (caspase 3 and caspase 9) and pseudo-cholinesterase (p.ChE) were performed. Also, we evaluated the apoptotic capacity through determining the genotoxic effects and chromosomal abnormalities among OP intoxicated patients. Results: We found the activity of caspases and serum MDA and TAC were significantly increased after OP poisoning and decreased after the appropriate atropine and oxime treatment course. The ROC curve suggested caspases as mortality and outcome predictive markers for acute OP poisoning patients. However, OP poisoning cases before treatment showed significant DNA damage, and they did not show any chromosomal aberration. Conclusion: The mentioned results strongly suggest apoptotic-related markers (caspase 3, caspase 9) as prognostic markers for evaluation of the treatment, outcomes, and mortality rate in the acute OP toxicity patients.

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“…Oxidative stress is a way for pesticide toxicity resulting in cell death (apoptosis and necrosis) and alters in vital and metabolic cell functions (Jacobsen‐Pereira et al, ; Soltaninejad & Abdollahi, ). The role of antioxidant enzymes and lipid peroxidation markers have been revealed in the pathogenesis of pesticides in several studies (Demir, Uzun, Durak, & Kalender, ).…”

Section: Discussionmentioning

confidence: 99%

Hematoprotective effect of vitamins C and E against subchronic toxicity of bendiocarb: Biochemical evidences

et al. 2018

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Oxidative stress parameters were tested in blood samples obtained from bendiocarb, vitamin C‐ and vitamin E‐treated rats and evaluated for lipid peroxidation and antioxidant enzymes. Hematologic parameters, ferric reducing antioxidant potential (FRAP) and trolox equivalent antioxidant capacity (TEAC) levels were also assayed in rat blood. The levels of malondialdehyde of erythrocytes and leucocytes, white blood cell (WBC), mean platelet volume (MPV) and platelet values increased, and glutathione peroxidase, superoxide dismutase, glutathione‐S‐transferase, catalase activities of erythrocytes and leucocytes, FRAP and TEAC values decreased in bendiocarb‐treated group. There were ameliorative effects of vitamins C and E on examining parameters. There were no significant differences between vitamin C, vitamin E and vitamin C + vitamin E treatment on changes caused by bendiocarb in GPx and GST activities, and WBC, MPV and platelet values. The present results suggest that reduced FRAP and TEAC values and antioxidant enzyme activities, enhanced lipid peroxidation, WBC, MPV, and platelet levels in the blood were handled in the context of oxidative stress. Practical applications Pesticides are used all over the world to remove agricultural pests. Pesticides which include the bendiocarb are cause to damage the cells by oxidative imbalance. Some of the determining parameters for oxidative damage are FRAP, TEAC, MDA, GST, GPx, SOD, CAT parameters. However, vitamins are very popular and commonly used antioxidants against xenobiotic. We also demonstrate that vitamins have been shown to protect against pesticide damage.

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Markers of genotoxicity and oxidative stress in farmers exposed to pesticides

Cited by 75 publications

References 52 publications

Hematological Abnormality, Oxidative Stress, and Genotoxicity Induction in the Greenhouse Pesticide Sprayers; Investigating the Role of NQO1 Gene Polymorphism

Hematological Abnormality, Oxidative Stress, and Genotoxicity Induction in the Greenhouse Pesticide Sprayers; Investigating the Role of NQO1 Gene Polymorphism

Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning

Hematoprotective effect of vitamins C and E against subchronic toxicity of bendiocarb: Biochemical evidences

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