Markers of Endothelial Cells in Normal and Pathological Conditions

Гончаров, Н. В.; Попова, П. И.; Avdonin, P. P.; Kudryavtsev, I. V.; Serebryakova, Maria; Корф, Е. А.; Авдонин, П. В.

doi:10.1134/s1990747819030140

Cited by 25 publications

(38 citation statements)

References 124 publications

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“…ECs are mainly identified by expressing cluster of differentiation (CD) 31 and von Willebrand factor (vWf) in co-cultures, while they express a variety of biomarkers including, Fli-1 (friend leukemia integration-1), CD13, CD29, CD36, CD34, CD39, CD44, CD47, ICAM-1 (intercellular adhesion molecule-1), CD61, CD62, CD80, CD102, CD105, CD106, CXCL16, CD143, CD144, CD146, ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs-13), ADAMTS18, and VE-cadherin (vascular endothelial cadherin). However, it must be taken into account that the ratio of expressed markers can be different in specialized endothelial cell networks ( Goncharov et al, 2020 ). In recent years, different sources of endothelial cells have been used alone or in a co-culture system to form capillary network in vitro .…”

Section: Endothelial Lineage Cellsmentioning

confidence: 99%

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Shafiee

Shariatzadeh

Zafari

et al. 2021

Front. Bioeng. Biotechnol.

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Currently, the fabrication of a functional vascular network to maintain the viability of engineered tissues is a major bottleneck in the way of developing a more advanced engineered construct. Inspired by vasculogenesis during the embryonic period, the in vitro prevascularization strategies have focused on optimizing communications and interactions of cells, biomaterial and culture conditions to develop a capillary-like network to tackle the aforementioned issue. Many of these studies employ a combination of endothelial lineage cells and supporting cells such as mesenchymal stem cells, fibroblasts, and perivascular cells to create a lumenized endothelial network. These supporting cells are necessary for the stabilization of the newly developed endothelial network. Moreover, to optimize endothelial network development without impairing biomechanical properties of scaffolds or differentiation of target tissue cells, several other factors, including target tissue, endothelial cell origins, the choice of supporting cell, culture condition, incorporated pro-angiogenic factors, and choice of biomaterial must be taken into account. The prevascularization method can also influence the endothelial lineage cell/supporting cell co-culture system to vascularize the bioengineered constructs. This review aims to investigate the recent advances on standard cells used in in vitro prevascularization methods, their co-culture systems, and conditions in which they form an organized and functional vascular network.

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Section: Endothelial Lineage Cellsmentioning

confidence: 99%

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Shafiee

Shariatzadeh

Zafari

et al. 2021

Front. Bioeng. Biotechnol.

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“…We investigated the expression of markers that are indicators of angiogenesis ( Figure 7 ); von Willebrand factor (vWF), platelet endothelial cell adhesion molecule-1 (PECAM-1), α-smooth muscle actin (α-SMA) and protein-tyrosine kinase receptor Flk-1, a receptor for VEGF. All of these are expressed in vascular endothelial cells [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…The molecular markers of angiogenesis are as follows; von Willebrand factor (vWF), one of the platelet coagulation factors that forms a platelet plug; platelet endothelial cell adhesion molecule-1 (PECAM-1), which accumulates at the vascular cell adhesion site and connects endothelial cells; α-smooth muscle actin (α-SMA), which is present in the smooth muscle cells of the blood vessel wall; and Flk-1, a VEGF receptor protein-tyrosine kinase [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy

Shimazaki

Noro

Hagikura

et al. 2021

Biology

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(1) Background: The control of angiogenesis is essential in disease treatment. We investigated angiogenesis-promoting or -suppressing factors and their molecular mechanisms. (2) Methods: Angiogenesis from HUVECs was quantitatively analyzed using the Angiogenesis Analysis Kit (Kurabo, Osaka, Japan). Human rAng-1-producing 107-35 CHO cells or mouse DFAT-D1 cells were co-cultured with HUVEC. Antioxidant polyphenols were added to the culture. Gene expression was analyzed by RT-PCR. (3) Results: The addition of rAng-1-producing cells, their culture supernatant, or commercially available rAng-1 showed a promoting effect on angiogenesis. The co-culture of DFAT-D1 cells promoted angiogenesis. Polyphenols showed a dose-dependent inhibitory effect on angiogenesis. Luteolin and quercetin showed remarkable anti-angiogenic effects. The expression of vWF, Flk1, and PECAM-1 was increased by adding rAng-1-producing cell culture supernatant. Polyphenols suppressed these genes. Apigenin and luteolin markedly suppressed α-SMA and Flk1. Resveratrol and quercetin enhanced the expression of PPARγ, and luteolin suppressed the expression of COX-1. The expression of endothelial nitric oxide synthase (eNOS), an oxidative stress-related gene, was slightly increased by luteolin. These results suggest that polyphenols induce ROS reduction. (4) Conclusions: We showed the promoting effect of Ang-1 or DFAT and the suppressing effect of polyphenols on angiogenesis and studied their molecular mechanisms. These results help control angiogenesis in regenerative therapy.

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“…This assumption was confirmed in the recent work of Raheel et al; according to their data, transcytosis of albumin in the skin—unlike in lungs—has saturation kinetics, which indicates a receptor-mediated process [ 197 ]. It was found that for albumin transcytosis through the endothelium of the microvessels of the dermis, the necessary and sufficient condition is the expression of the CD36 glycoprotein, which is known as the scavenger receptor class 3B (SR-B3), platelet membrane glycoprotein IV (GPIV), glycoprotein IIIb (GPIIIb), thrombospondin receptor, collagen receptor, FA translocase (FAT), and even as an innate immune receptor [ 198 ]. Upon ligand binding, CD36 triggers a signaling cascade that mediates a wide range of pro-inflammatory responses.…”

Section: Interaction Of Albumin With Ec: Glycocalyx Transcytosis and Glycoprotein Cd36mentioning

confidence: 99%

Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties

Belinskaia

Voronina

Шмурак

et al. 2021

IJMS

104

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Being one of the main proteins in the human body and many animal species, albumin plays a decisive role in the transport of various ions—electrically neutral and charged molecules—and in maintaining the colloidal osmotic pressure of the blood. Albumin is able to bind to almost all known drugs, as well as many nutraceuticals and toxic substances, largely determining their pharmaco- and toxicokinetics. Albumin of humans and respective representatives in cattle and rodents have their own structural features that determine species differences in functional properties. However, albumin is not only passive, but also an active participant of pharmacokinetic and toxicokinetic processes, possessing a number of enzymatic activities. Numerous experiments have shown esterase or pseudoesterase activity of albumin towards a number of endogeneous and exogeneous esters. Due to the free thiol group of Cys34, albumin can serve as a trap for reactive oxygen and nitrogen species, thus participating in redox processes. Glycated albumin makes a significant contribution to the pathogenesis of diabetes and other diseases. The interaction of albumin with blood cells, blood vessels and tissue cells outside the vascular bed is of great importance. Interactions with endothelial glycocalyx and vascular endothelial cells largely determine the integrative role of albumin. This review considers the esterase, antioxidant, transporting and signaling properties of albumin, as well as its structural and functional modifications and their significance in the pathogenesis of certain diseases.

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Markers of Endothelial Cells in Normal and Pathological Conditions

Cited by 25 publications

References 124 publications

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy

Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties

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