Markers of Connective Tissue Degradation in the Small Intestine of Rats Undergoing Surgical Trauma Replicated Under Experimental Post-Traumatic Stress Disorder

Abstract

The objective of this experiment is to study the indices of the depolymerisation of biopolymers in the small intestine connective tissue of rats undergoing surgical trauma replicated under post-traumatic stress disorder. The study was carried out on 42 white rats. The findings obtained have shown that the experimental modelling of posttraumatic stress disorder (single prolonged stress) significantly elevates the concentration of free hydroxyproline (by 44.9%, P<0. 001), hexuronic acids (by 47.0%, P<0.001), and N-acetylneuraminic acid (by 46.2%, P<0.001), indicating depolymerisation of the extracellular matrix components, collagen, proteoglycans, and sialoglycoproteins, in this organ. On the 7th day after laparotomy performed under the experimental model of posttraumatic stress disorder, the content of free hydroxyproline, hexuronic acids, and N-acetylneuraminic acid in the small intestine homogenate significantly exceeded the respective values obtained after the separate laparotomy and "sham" surgical operation performed under simulated single prolonged stress.