Markers for the Characterization of Liver Mesenchymal Stem Cell

Lotfy, Ahmed

doi:10.19080/ijcsmb.2019.06.555676

Cited by 2 publications

(4 citation statements)

References 37 publications

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“…But there were some findings mentioned by (13) in rabbits cleared CD105 was absent from rabbit MSCs, besides ( 9) and (10) in humans. The positive expression of CD106 was similar to previous reports in humans (4). While (13) in rabbits disagreed with CD106 positive expression, they confirmed that CD106 was absent from the rabbit and human MSCs.…”

Section: Flow Cytometrysupporting

confidence: 87%

“…MSCs/stromal stem cells were first isolated from the bone marrow (4). Although the bone marrow was considered the common source for MSCs, they could be isolated from other sources, taking into consideration the differences in surface markers, proliferation rates, and differentiation ability.…”

Section: Introductionmentioning

confidence: 99%

“…Although the bone marrow was considered the common source for MSCs, they could be isolated from other sources, taking into consideration the differences in surface markers, proliferation rates, and differentiation ability. Many types of research were directed to isolate and characterize LMSCs to draw attention to the liver as an alternative source for MSCs, particularly due to increase demand for liver therapies and transplantation (4). LMSCs exhibited a distinct morphology and expressed specific sets of CD molecules.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of Fetal and Neonatal Liver-Derived Mesenchymal Stem Cells From Rabbits

Elsheikh,

EL-Hady,

Abdallah

et al. 2021

SVR

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Mesenchymal stem cells (MSCs) became in recent years a promising implement for cell therapies. Our study described the isolation and characterization of liver mesenchymal stem cells (LMSCs). We isolated LMSCs from the rabbit liver tissue from two groups: 20th day's old fetuses and 4 days-old kittens. LMSCs were characterized by flow cytometry, immunohistochemistry (IHC), and quantitative real-time-polymerase chain reaction (qRT-PCR). Flow cytometry of the isolated LMSCs showed variable expression of the cluster of differentiation markers (CD) including, CD105, CD106, CD11b, CD38, CD63, CD9, and CD14. Fetal liver isolated LMSCs showed expression of CD105, CD106, CD9, and CD63 but no expression of CD14, CD11b, and CD38 was observed. Absolute expression of LMSCs was performed by qRT-PCR analysis to detect cytoskeleton genes including, vimentin, nestin, and desmin in the experimental groups. In vivo localization of stem cell markers such as CD105 (Endoglin), vimentin, and desmin was also distinguished in fetal and neonatal liver tissues using IHC.

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Section: Flow Cytometrysupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Characterization of Fetal and Neonatal Liver-Derived Mesenchymal Stem Cells From Rabbits

Elsheikh,

EL-Hady,

Abdallah

et al. 2021

SVR

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“…These cells express mesenchymal markers such as CD29, CD44, CD73, CD90, HLA-Class I, etc. [32]. Still, the expression levels of these markers vary in reports from different authors.…”

Section: Morphology and Phenotype Of Human Liver Mscsmentioning

confidence: 99%

Mesenchymal Stem Cells in the Adult Human Liver: Hype or Hope?

Холоденко

Курбатов

Холоденко

et al. 2019

Cells

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Chronic liver diseases constitute a significant economic, social, and biomedical burden. Among commonly adopted approaches, only organ transplantation can radically help patients with end-stage liver pathologies. Cell therapy with hepatocytes as a treatment for chronic liver disease has demonstrated promising results. However, quality human hepatocytes are in short supply. Stem/progenitor cells capable of differentiating into functionally active hepatocytes provide an attractive alternative approach to cell therapy for liver diseases, as well as to liver-tissue engineering, drug screening, and basic research. The application of methods generally used to isolate mesenchymal stem cells (MSCs) and maintain them in culture to human liver tissue provides cells, designated here as liver MSCs. They have much in common with MSCs from other tissues, but differ in two aspects—expression of a range of hepatocyte-specific genes and, possibly, inherent commitment to hepatogenic differentiation. The aim of this review is to analyze data regarding liver MSCs, probably another type of liver stem/progenitor cells different from hepatic stellate cells or so-called hepatic progenitor cells. The review presents an analysis of the phenotypic characteristics of liver MSCs, their differentiation and therapeutic potential, methods for isolating these cells from human liver, and discusses issues of their origin and heterogeneity. Human liver MSCs are a fascinating object of fundamental research with a potential for important practical applications.

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Markers for the Characterization of Liver Mesenchymal Stem Cell

Cited by 2 publications

References 37 publications

Isolation and Characterization of Fetal and Neonatal Liver-Derived Mesenchymal Stem Cells From Rabbits

Isolation and Characterization of Fetal and Neonatal Liver-Derived Mesenchymal Stem Cells From Rabbits

Mesenchymal Stem Cells in the Adult Human Liver: Hype or Hope?

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