Markers for Plant Gene Transfer

Bowen, Ben

doi:10.1016/b978-0-12-428781-5.50031-4

Cited by 39 publications

(21 citation statements)

References 229 publications

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“…As non-transformed horse chestnut roots did not grow in PGR-free medium, we avoided using antibiotics or herbicides as selective agents, which is a major advantage as these generally allow the forming of chimera and have negative impact on proliferation and differentiation (James and Dandekar 1991). Most explant cells die during negative selection, which may cause a release of toxic substances (such as phenolics) that may affect transformed cells (Bowen 1992). Moreover, the transformation frequencies obtained by positive selection appear to be higher than those obtained by kanamycin selection (Joersbo and Okkels 1996).…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

et al. 2004

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Hairy roots were induced from androgenic embryos of horse chestnut (Aesculus hippocastanum L.) by infection with Agrobacterium rhizogenes strain A4GUS. Single roots were selected according to their morphology in the absence of antibiotic or herbicide resistance markers. Seventy-one putative transformed hairy root lines from independent transformation events were established. Regeneration was induced in MS liquid medium supplemented with 30 microM 6-benzylaminopurine (BA), and the regenerants were multiplied on MS solid medium containing 10 microM BA. Following elongation on MS medium supplemented with 1 microM BA and 500 mg/l polyvinylpyrrolidone, the shoots were subjected to a root-inducing treatment. Stable integration of TL-DNA within the horse chestnut genome was confirmed by Southern hybridization. The copy number of transgenes was estimated to be from two to four.

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Section: Discussionmentioning

confidence: 99%

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

et al. 2004

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“…The effectiveness of tissue-culture selection regimes is dependent on many factors, including type of explant, the chemical properties of the selective agents, and time of application (Bowen 1993). Usually, higher selection pressure enhances the selection efficiency, but the regeneration ability will also be reduced, making it difficult to regenerate plantlets after selection.…”

Section: Discussionmentioning

confidence: 99%

Establishment of an Agrobacterium-mediated transformation system for Periploca sepium Bunge

Ren

Gyokusen

Nakazawa

et al. 2010

Plant Biotechnology

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“…Cereal plants with chimeric sectors were generated using direct DNA delivery into shoot apical meristems such as maize (Lusardi et al, 1994). Even, large transgenic sectors that extend through the ear node and into the tassel of mature maize plants failed to produce transgenic progeny (Bowen, 1993).…”

Section: Tissue Culture and Plant Chimerasmentioning

confidence: 99%

Genetic transformation of Sorghum bicolor

Girijashankar

Swathisree

2009

Physiol Mol Biol Plants

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Great millet (Sorghum bicolor (L.) Moench) is cultivated across the world for food and fodder. It is typically grown in semiarid regions that are not suitable for cultivation of other major cereals. Sexual incompatibility and shortage of available genes in germplasm to combat biotic and abiotic stresses resulted in marginalized yields of this crop. Genetic modification of sorghum with agronomically useful genes can address this problem. Here, we tried to review and summarize the key aspects of sorghum transformation work being carried out so far by various research groups across the world. The approaches used and the obstacles in generating transgenic sorghum are also pointed out and discussed.

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Markers for Plant Gene Transfer

Cited by 39 publications

References 229 publications

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

Establishment of an Agrobacterium-mediated transformation system for Periploca sepium Bunge

Genetic transformation of Sorghum bicolor

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