Marker profile of mesothelial cells <i>versus</i> ovarian carcinoma cells

Niekerk, Catharina C. van; Jap, P. H. K.; Thomas, Chris M.G.; Smeets, D.F.C.M.; Ramaekers, Frans C. S.; Poels, L. G.

doi:10.1002/ijc.2910430619

Cited by 28 publications

(10 citation statements)

References 22 publications

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“…However, since the initial report of Bast et al [3], several studies have demonstrated CA-125 positivity in the benign epithelium of the female genital tract, and also in the normal lung, serous membranes, various inflammatory processes, and malignant mesotheliomas [4,9,19,27,28]. In contrast, no CA-125 positivity was found in adult ovaries [37], and weak to negative staining was seen in cultured mesothelial cells [36]. Most of the above studies have not used antigen demasking of any kind, and the reported pattern of staining in these studies is either cytoplasmic or membranous.…”

Section: Discussionmentioning

confidence: 91%

Detection of cancer cells in effusions from patients diagnosed with gynaecological malignancies

Davidson¹,

Risberg²,

Kristensen

et al. 1999

Virchows Archiv

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The detection of malignant cells in pleural, peritoneal, and pericardial fluids of cancer patients marks the presence of metastatic disease and is associated with a grave prognosis. We evaluated five epithelial markers for the detection of cancer cells in 94 fresh pleural, peritoneal and pericardial effusions. Eighty-four of the samples were regarded as adequate for analysis after evaluation of cytological smears, including 61 samples from patients known to have gynaecological neoplasms. The other 23 samples were from patients with various non-gynaecological malignancies or tumours of unknown origin. Our control cases were 10 fallopian tubes not affected by any malignancy and 12 malignant mesotheliomas. Cell blocks from all cases were stained for CA-125, BerEP4, carcinoembryonic antigen (CEA), BG8 (Lewis Y blood antigen), and B72.3 (TAG-72). Fifty-one of 84 cases were diagnosed as malignant or suggestive of malignancy in cytological smears and/or cell block sections. However, staining for epithelial markers highlighted the presence of malignant cells in 7 additional cases. When membrane staining was evaluated, the sensitivity of the markers studied in detecting malignant cells was as follows: CA-125: 88%, BerEP4: 78%, CEA: 26%, BG8: 86%, B72.3: 79%. Membrane positivity for CEA, B72.3 and BerEP4 was not detected in reactive mesothelial cells. However, membranous staining in mesothelial cells was evident in 13% and 31% of cases with the use of BG8 and CA-125, respectively. Weak cytoplasmic staining for CEA was observed in mesothelial cells in 2 cases. When Ber-EP4, B72.3, and BG8 staining results in cancer cells were combined, the following sensitivity levels were observed: BG8+B72.3: 91%; BG8+Ber-EP4: 90%; B72.3+Ber-EP4: 93%; BG8+Ber-EP4+B72.3: 95%. The detection of malignant cells in effusions is facilitated by the use of immunocytochemistry using a wide panel of antibodies. BerEP4 and B72.3 appear to be the best markers when both sensitivity and specificity are considered, followed by BG8, while CEA and CA-125 have a limited role in the detection of metastases from gynaecological tumours owing to the low sensitivity of the former and the low specificity of the latter. Analysis of all staining results should be based on a thorough morphological examination.

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Section: Discussionmentioning

confidence: 91%

Detection of cancer cells in effusions from patients diagnosed with gynaecological malignancies

Davidson¹,

Risberg²,

Kristensen

et al. 1999

Virchows Archiv

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“…31,37,38 The OV-TL 12/30 clone of CK7 antibody was described as a chain-specific monoclonal antibody that stains intermediate filament structures. 39 Moreover, OV-TL 12/30 antibody was found to specifically react with CK7 on electrophoretically separated cytoskeletal preparations (one-and two-dimensional immunoblots) of human cell lines. 40 Differences in the reactivity patterns of individual CK7 antibodies were explained as the likely result of epitope masking.…”

Section: Discussionmentioning

confidence: 98%

The OV-TL 12/30 Clone of Anti-cytokeratin 7 Antibody as a New Marker of Corneal Conjunctivalization in Patients with Limbal Stem Cell Deficiency

Jirsová

Ďuďáková²,

Kalasova³

et al. 2011

Invest. Ophthalmol. Vis. Sci.

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“…We have thus concluded that the increase in CA 125 in these breast cancer patients was caused by the metastatic infiltration of either the lung or the pleura: only in those patients with pleural involvement were CA 125 levels elevated; CA 125 levels in patients with no pleural involvement remained low. This hypothesis is further substantiated by reports indicating that CA 125 levels are elevated in patients with primary lung cancer predominantly when the pleura is affected (Niwa & Shimokata, 1986, Van Niekerk et al, 1989Kandylis et al, 1990;Kimura et al, 1990;Diez et al, 1991).…”

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confidence: 73%

“…Elevated CA 125 levels in primary lung cancer have been reported (Niwa & Shimokata, 1986;Van Niekerk et at., 1989; (0), liver (0) and lung (0) metastases, at the time of detection and thereafter. The CA 125 levels are shown only for those patients with elevated CA 125 levels at the time of detection of metastases.…”

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confidence: 99%

Is an increase in CA 125 in breast cancer patients an indicator of pleural metastases?

Jäger

Kissing²,

Cilaci³

et al. 1994

Br J Cancer

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S_lThe retrospective analysis of 250 breast cancer patients with disseminated disease provided evidence that the increase in CA 125 serum levels in these patients was caused by lung metastases or pleural effusions. Seven patients with lung metastases and pleural involvement had elevated CA 125 levels, while in four patients with lung metastases but without pleural effusions CA 125 levels remained normal. In patients with only bone or liver metastases ResultsOf a group of 250 patients with disseminated breast cancer, only 26 fulfilled the inclusion criteria. Most patients were excluded because multiple metastases were detected or could not be ruled out within 6 months after the detection of the first metastases. The final distribution of isolated metastases in these 26 patients was as follows: bone = nine patients (premenopausal: two patients), liver = six patients (premenopausal: four patients) and lung= 11 patients (premenopausal: five patients).The CA 125 levels at the time of detection of these metastases are shown in Figure 1. As can be seen, 7 of the 11 patients with lung metastases had elevated CA 125 serum levels (three premenopausal and four post-menopausal patients), while only one of nine patients with bone metastases (post-menopausal patient) and one out of six patients with liver metastases had high CA 125 levels (post-menopausal patient). Of the seven patients with lung metastases and elevated CA 125 levels, five had pleural effusions when the metastases were detected. The other two patients developed a pleuritis carcinomatosa with pleural effusions within 4 weeks of the diagnosis of the lung metastasis. The remaining four patients with normal CA 125 levels did not develop pleural effusions. The patient with high CA 125 levels and bone metastasis was shown post mortem (7 months later) to have pleuritis carcinomatosa, which had not been previously detected. The serial CA 125 measurements showed that in some patients the CA 125 concentration increased months before the detection of the metastasis (Figure 2). In the one patient with a liver metastasis and elevation of CA 125, the endocrine and metabolic function of the liver was impaired. In this patient serum aspartate transaminase (AST), serum alanine transaminase (ALT) and serum glutamyl transferase (GGT) were elevated.

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Marker profile of mesothelial cells versus ovarian carcinoma cells

Cited by 28 publications

References 22 publications

Detection of cancer cells in effusions from patients diagnosed with gynaecological malignancies

Detection of cancer cells in effusions from patients diagnosed with gynaecological malignancies

The OV-TL 12/30 Clone of Anti-cytokeratin 7 Antibody as a New Marker of Corneal Conjunctivalization in Patients with Limbal Stem Cell Deficiency

Is an increase in CA 125 in breast cancer patients an indicator of pleural metastases?

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