Marker-free Detection on Microarrays

Vaupel, M.; Eing, Andreas; Greulich, Karl-Otto; Roegener, Jan; Schellenberg, Peter; Striebel, Hans‐Martin; Arlinghaus, Heinrich F.

doi:10.1007/3-540-26578-3_9

Cited by 4 publications

(1 citation statement)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SPPs are already being widely used to enhance Raman scattering [77], molecular fluorescence [78] and the output efficiency of solid-state light emitters [79] and terahertz lasers [80]. Commercial SPP-based ellipsometers [81] are capable of tracing minute amounts of substance, e.g., in the detection of protein monolayers or to monitor antibody reactions [82]. Field localization properties of SPPs have also recently been used to demonstrate trapping of micro-colloids on a patterned metal surface under low laser intensity.…”

Section: Ultra-fast Surface Plasmon Polariton Nano-opticsmentioning

confidence: 99%

Ultra‐fast nano‐optics

Vasa

Ropers

Pomraenke

et al. 2009

Laser & Photonics Reviews

View full text Add to dashboard Cite

Ultra-fast nano-optics is a comparatively young and rapidly growing field of research aiming at probing, manipulating and controlling ultrafast optical excitations on nanometer length scales. This ability to control light on nanometric length and femtosecond time scales opens up exciting possibilities for probing dynamic processes in nanostructures in real time and space. This article gives a brief introduction into the emerging research field of ultrafast nano-optics and discusses recent progress made in it. A particular emphasis is laid on the recent experimental work performed in the authors' laboratories. We specifically discuss how ultrafast nano-optical techniques can be used to probe and manipulate coherent optical excitations in individual and dipole-coupled pairs of quantum dots, probe the dynamics of surface plasmon polariton excitations in metallic nanostructures, generate novel nanometer-sized ultrafast light and electron sources and reveal the dipole interaction between excitons and surface plasmon polaritons in hybrid metal-semiconductor nanostructures. Our results indicate that such hybrid nanostructures carry significant potential for realizing novel nano-optical devices such as ultrafast nano-optical switches as well as surface plasmon polariton amplifiers and lasers. Two-dimensional finite difference time domain (FDTD) simulation of the spatio-temporal evolution of a 10 fs light pulse at a center wavelength of 810 nm propagating through a tapered, perfectly conducting metal-coated fiber probe of 100 nm aperture diameter. The field intensity |Ex(x, y, t)| 2 is displayed on a logarithmic intensity scale at four different instants in time. After t ∼ 14 fs the pulse center reaches the aperture, generating directly below it an ultra-short near-field spot of light.

show abstract

Section: Ultra-fast Surface Plasmon Polariton Nano-opticsmentioning

confidence: 99%

Ultra‐fast nano‐optics

Vasa

Ropers

Pomraenke

et al. 2009

Laser & Photonics Reviews

View full text Add to dashboard Cite

show abstract

Survey of the year 2005 commercial optical biosensor literature

2006

View full text Add to dashboard Cite

We identified 1113 articles (103 reviews, 1010 primary research articles) published in 2005 that describe experiments performed using commercially available optical biosensors. While this number of publications is impressive, we find that the quality of the biosensor work in these articles is often pretty poor. It is a little disappointing that there appears to be only a small set of researchers who know how to properly perform, analyze, and present biosensor data. To help focus the field, we spotlight work published by 10 research groups that exemplify the quality of data one should expect to see from a biosensor experiment. Also, in an effort to raise awareness of the common problems in the biosensor field, we provide side-by-side examples of good and bad data sets from the 2005 literature.

show abstract

Optical technologies for the read out and quality control of DNA and protein microarrays

Schäferling

Nagl

2006

Anal Bioanal Chem

View full text Add to dashboard Cite

Microarray formats have become an important tool for parallel (or multiplexed) monitoring of biomolecular interactions. Surface-immobilized probes like oligonucleotides, cDNA, proteins, or antibodies can be used for the screening of their complementary targets, covering different applications like gene or protein expression profiling, analysis of point mutations, or immunodiagnostics. Numerous reviews have appeared on this topic in recent years, documenting the intriguing progress of these miniaturized assay formats. Most of them highlight all aspects of microarray preparation, surface chemistry, and patterning, and try to give a systematic survey of the different kinds of applications of this new technique. This review places the emphasis on optical technologies for microarray analysis. As the fluorescent read out of microarrays is dominating the field, this topic will be the focus of the review. Basic principles of labeling and signal amplification techniques will be introduced. Recent developments in total internal reflection fluorescence, resonance energy transfer assays, and time-resolved imaging are addressed, as well as non-fluorescent imaging methods. Finally, some label-free detection modes are discussed, such as surface plasmon microscopy or ellipsometry, since these are particularly interesting for microarray development and quality control purposes.

show abstract

Marker-free Detection on Microarrays

Cited by 4 publications

References 50 publications

Ultra‐fast nano‐optics

Ultra‐fast nano‐optics

Survey of the year 2005 commercial optical biosensor literature

Optical technologies for the read out and quality control of DNA and protein microarrays

Contact Info

Product

Resources

About