Marker assisted separation of resistance genes Rph22 and Rym16 Hb from an associated yield penalty in a barley: Hordeum bulbosum introgression line

Johnston, Paul A.; Meiyalaghan, Vijitha; Forbes, M.; Habekuß, Antje; Butler, Ruth; Pickering, R. A.

doi:10.1007/s00122-015-2495-z

Cited by 12 publications

(7 citation statements)

References 68 publications

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“…2HL. Recently, Johnston et al (2015) has demonstrated the usefulness of a similar approach using overlapping Hb segments to generate ILs with particular small introgression sizes.…”

Section: Strategy To Avoid Low Interspecific Recombinationmentioning

confidence: 99%

Bulbosum to Go: A Toolbox to Utilize Hordeum vulgare/bulbosum Introgressions for Breeding and Beyond

et al. 2015

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Hordeum bulbosum L., a wild relative of barley (Hordeum vulgare L.), has been considered as a valuable source of genetic diversity for barley improvement. Since the 1990s, a considerable number of barley/H. bulbosum introgression lines (IL)s has been generated, with segments introgressed from H. bulbosum harboring a diverse set of desirable traits. However, the efficient utilization of these ILs has been hampered, largely due to the lack of suitable molecular tools for their genetic characterization and highly reduced interspecific recombination frequencies in the region of the introgression. In the present study, we utilized genotyping-by-sequencing for the detailed molecular characterization of 145 ILs. Genotypic information allows the genetic diversity within the set of ILs to be determined and a strategy was outlined to tackle the obstacle of reduced recombination frequencies. Furthermore, we compiled exome capture re-sequencing information of barley and H. bulbosum and designed an integrated barley/H. bulbosum sequence resource with polymorphism information on interspecific and intraspecific sequence variations of both species. The integrated sequence will be valuable for marker development in barley/H. bulbosum ILs derived from any barley and H. bulbosum donors. This study provides the tools for the widespread utilization of barley/H. bulbosum ILs in applied barley breeding and academic research.

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“…2HL. Recently, Johnston et al (2015) has demonstrated the usefulness of a similar approach using overlapping Hb segments to generate ILs with particular small introgression sizes.…”

Section: Strategy To Avoid Low Interspecific Recombinationmentioning

confidence: 99%

Bulbosum to Go: A Toolbox to Utilize Hordeum vulgare/bulbosum Introgressions for Breeding and Beyond

et al. 2015

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“…In addition to cloned eIF4E and PDIL5-1 , rym7 [ 26 ], Rym16 HB [ 27 , 28 ], Rym17 [ 29 ] and Rym14 HB [ 4 ] have been fine-mapped to a small region on chromosome 1H, 2H, 3H, and 6H, respectively. The remaining 10 BYMD-resistant genes rym8 [ 30 ], rym9 [ 31 ], rym12 [ 32 ], rym13 [ 33 ], rym18 [ 29 ], rym3 [ 34 ], Un-designated [ 35 ], rym15 [ 36 ], rym2 [ 32 ] and rym7t [ 37 ] have been preliminarily mapped.…”

Section: Backgroundsmentioning

confidence: 99%

Identification of novel QTL contributing to barley yellow mosaic resistance in wild barley (Hordeum vulgare spp. spontaneum)

et al. 2021

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Background Barley yellow mosaic disease (BYMD) caused by Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) seriously threatens the production of winter barley. Cultivating and promoting varieties that carry disease-resistant genes is one of the most powerful ways to minimize the disease’s effect on yield. However, as the BYMD virus mutates rapidly, resistance conferred by the two cloned R genes to the virus had been overcome by new virus strains. There is an urgent need for novel resistance genes in barley that convey sustainable resistance to newly emerging virus strains causing BYMD. Results A doubled haploid (DH) population derived from a cross of SRY01 (BYMD resistant wild barley) and Gairdner (BYMD susceptible barley cultivar) was used to explore for QTL of resistance to BYMD in barley. A total of six quantitative trait loci (qRYM-1H, qRYM-2Ha, qRYM-2Hb, qRYM-3H, qRYM-5H, and qRYM-7H) related to BYMD resistance were detected, which were located on chromosomes 1H, 2H, 3H, 5H, and 7H. Both qRYM-1H and qRYM-2Ha were detected in all environments. qRYM-1H was found to be overlapped with rym7, a known R gene to the disease, whereas qRYM-2Ha is a novel QTL on chromosome 2H originated from SRY01, explaining phenotypic variation from 9.8 to 17.8%. The closely linked InDel markers for qRYM-2Ha were developed which could be used for marker-assisted selection in barley breeding. qRYM-2Hb and qRYM-3H were stable QTL for specific resistance to Yancheng and Yangzhou virus strains, respectively. qRYM-5H and qRYM-7H identified in Yangzhou were originated from Gairdner. Conclusions Our work is focusing on a virus disease (barley yellow mosaic) of barley. It is the first report on BYMD-resistant QTL from wild barley accessions. One novel major QTL (qRYM-2Ha) for the resistance was detected. The consistently detected new genes will potentially serve as novel sources for achieving pre-breeding barley materials with resistance to BYMD.

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“…However, this performance under stress conditions should not be at the cost of yield under control or non-submergence conditions, since flooding usually occur once in 2–5 years. Previous studies have reported contrasting results showing favorable, unfavorable or even neutral effects of different genes incorporated in various crop species 28 , 29 . However, replete knowledge on the influence of Sub1 QTL on yield and yield related parameters in farmers’ fields not affected by floods is incomplete.…”

Section: Introductionmentioning

confidence: 96%

No yield penalty under favorable conditions paving the way for successful adoption of flood tolerant rice

Dar¹,

Zaidi²,

Waza³

et al. 2018

Sci Rep

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Flooding is one of the major constraints for rice production in rainfed lowlands, especially in years and areas of high rainfall. Incorporating the Sub1 (Submergence1) gene into high yielding popular varieties has proven to be the most feasible approach to sustain rice production in submergence-prone areas. Introgression of this QTL into popular varieties has resulted in considerable improvement in yield after flooding. However, its impact under non-flooded conditions or years have not been thoroughly evaluated which is important for the farmers to accept and adopt any new version of their popular varieties. The present study was carried out to evaluate the effect of Sub1 on grain yield of rice in different genetic backgrounds, under non-submergence conditions, over years and locations. The study was carried out using head to head trials in farmer’s fields, which enable the farmers to more accurately compare the performance of Sub1 varieties with their recurrent parents under own management. The data generated from different head to head trials revealed that the grain yield of Sub1 varieties was either statistically similar or higher than their non-Sub1 counterparts under non-submergence conditions. Thus, Sub1 rice varieties show no instance of yield penalty of the introgressed gene.

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Marker assisted separation of resistance genes Rph22 and Rym16 Hb from an associated yield penalty in a barley: Hordeum bulbosum introgression line

Cited by 12 publications

References 68 publications

Bulbosum to Go: A Toolbox to Utilize Hordeum vulgare/bulbosum Introgressions for Breeding and Beyond

Bulbosum to Go: A Toolbox to Utilize Hordeum vulgare/bulbosum Introgressions for Breeding and Beyond

Identification of novel QTL contributing to barley yellow mosaic resistance in wild barley (Hordeum vulgare spp. spontaneum)

No yield penalty under favorable conditions paving the way for successful adoption of flood tolerant rice

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