Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight

Ni, Dahu; Song, Fengshun; Ni, Jianfeng; Zhang, Aifang; Wang, Chunlian; Zhao, Kun; Yang, Yaolong; Wei, Pengcheng; Yang, Jian‐Bo; Li, Li

doi:10.1016/j.fcr.2015.07.018

Cited by 44 publications

(25 citation statements)

References 26 publications

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“…In the past decade, breeders have paid more attention to super two-line hybrid rice for increasing yields (Yuan 2017 ). During the past decade, the proportion of planting area for two-line hybrid rice has significantly increased by 25% in China (Mou 2016 ; Ni et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Yang

Chen

et al. 2018

Rice

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BackgroundBreeding two-line hybrid rice with disease resistance is an effective approach to stabilize rice yield in commercial rice production of China.ResultsWe improved the blast and bacterial blight resistance of Guangzhan63-4S, an elite photoperiod- and thermo-sensitive male sterile (P/TGMS) line widely used in two-line hybrid rice, by introducing the R genes Pi2 and Xa7 conferring resistance to rice blast and bacterial blight, respectively. Through the backcrossing and gene pyramiding breeding coupled with molecular marker-assisted selection, a new P/TGMS line Hua1228S carrying Pi2, Xa7, and tms5 was developed. Based on 200,000 SNP markers by next-generation sequencing, Hua1228S covered 87.6% of the recurrent genome, as well as 4.5% of the donor genome from VE6219 and 7.9% from YR7029–39. When infected with seven tested Xanthomonas oryzae pv. oryzae strains, Hua1228S conferred high resistance (0 level) to six bacterial blight strains. Moreover, Hua1228S showed broad-spectrum resistance to rice blast isolates with a high resistance frequency of 90.91%. High levels of resistance to leaf blast and neck blast were observed under heavy disease pressure in natural field. Importantly, Hua1228S showed identical fertility-sterility alteration pattern to Guangzhan63-4S. Thus, two hybrid combinations Hua Liangyou 2821 and Hua Liangyou 284 derived from Hua1228S exhibited enhanced resistance and higher yield compared with the control variety Feng Liangyou 4.ConclusionsThese results indicate that Hua1228S has tremendous potentiality to increase and stabilize the rice yield, through the introgression of two R genes by marker-assisted selection strategy.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0203-8) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Yang

Chen

et al. 2018

Rice

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“…The rice blast R gene Pi9 in the indica rice line 75-1-127 was introgressed from the wild species Oryza minuta (Amante-Bordeos et al 1992; Liu et al 2002) . The Pi9 gene conferred broad-spectrum disease resistance to 43 M. oryzae isolates collected from 13 countries (Liu et al 2002) and was used in rice breeding program (Khanna et al 2015; Koide et al 2011; Luo and Yin 2013; Ni et al 2015). …”

Section: Introductionmentioning

confidence: 99%

Marker-assisted breeding of the rice restorer line Wanhui 6725 for disease resistance, submergence tolerance and aromatic fragrance

Luo

Zhang

et al. 2016

Rice

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BackgroundRice is a staple food crop in the world. With the increase in world population and economic development, farmers need to produce more rice in limited field. However, the rice production is frequently affected by biotic and abiotic stresses. The use of natural disease resistance and stress tolerance through genetic breeding is the most efficient and economical way to combat or acclimate to these stresses. In addition, rice with aromatic fragrance can significantly increase market value for its good grain quality. Mianhui 725 (MH725) is an elite restorer line that has been widely used to produce three-line hybrid rice in China. We previously introduced rice bacterial blight resistance genes Xa4 and Xa21 into MH725 and obtained an introgression rice line Wanhui 421 (WH421), which theoretically possesses 96.9% genetic background of MH725.ResultsHere we report the introduction and pyramiding of disease resistance genes Xa27 and Pi9, submergence tolerance gene Sub1A and aromatic fragrance gene badh2.1 in WH421 through backcrossing and marker-assisted selection. The newly developed introgression rice line was designated as Wanhui 6725 (WH6725), which theoretically possesses 95.0% genetic background of MH725. WH6725 and its hybrid rice conferred disease resistance to both blast and bacterial blight diseases and showed tolerance to submergence for over 14 days without significant loss of viability. Compared with non-aromatic rice MH725, WH6725 has strong aromatic fragrance. The major important agronomic traits and grain quality of WH6725 and its hybrid rice obtained in field trials were similar to those of MH725 and the control hybrid rice, indicating that WH6725 is as good as MH725 when it is used as a restorer line for three-line hybrid rice production.ConclusionWe have successfully developed a new restorer line WH6725 with disease resistance to rice blast and bacterial blight, tolerance to submergence and aromatic fragrance, which can be used to replace MH725 for hybrid rice production.

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“…Therefore, rice blast resistance has been considered as one of the priorities in the rice breeding program. R-gene linked or specific molecular markers were developed and widely used in the mainstream breeding programs via marker aided selection (MAS) (Jiang et al 2012;Balachiranjeevi et al 2015;Ni et al 2015;Shalini et al 2016;Wu et al 2016;Mi et al 2018). However, many rice blast R-gene haplotypes from different rice lines are almost identical except for a few nucleotide differences, leading to different specificities or even loss of function, e.g, different Pik and Pi2/9 alleles (Zhou et al 2007;Constanzo and Jia 2010).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

Xiao

Jiao

Zhu

et al. 2020

Rice

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Multiple haplotypes at the same rice blast R-gene locus share extremely high sequence similarity, which makes the gene diagnostic method using molecular markers less effective in differentiation from one another. The composition and distribution pattern of deployed R genes/haplotypes in elite rice varieties has not been extensively analyzed. In this study, we employed PCR amplification and sequencing approach for the diagnosis of R-gene haplotypes in 54 Chinese elite rice varieties. A varied number of functional and nonfunctional haplotypes of 4 target major R-gene loci, i.e., Pi2/9, Pi5, Pik, and Pib, were deduced by referring to the reference sequences of known R genes. Functional haplotypes accounted for relatively low frequencies for the Pi2/9 (15%) and Pik (9%) loci but for relatively high frequencies for the Pi5 (50%) and Pib (54%) loci. Intriguingly, significant frequencies of 33%, 39%, 46% of non-functional haplotypes at the Pi2/9, Pik, and Pib loci, respectively, with traceable original donors were identified, suggesting that they were most likely unintentionally spread by using undesirable donors in various breeding programs. In the case of Pi5 locus, only a single haplotype, i.e., Pi5 was identified. The reactions of 54 rice varieties to the differential isolates were evaluated, which showed a good correlation to the frequency of cognate avirulence (Avr) genes or haplotypes in the differential isolates. Four R genes, i.e., Pi2, Piz-t, Pi50, and Pikm were found to contribute significantly to the resistance of the elite rice varieties. Other two genes, Pi9 and Pikh, which were not utilized in rice varieties, showed promising values in breeding durable resistance due to their high resistance frequencies to the contemporary rice blast population. The sequencebased molecular diagnosis provided a promising approach for the identification and verification of haplotypes in different R-gene loci and effective R genes valuable for breeding durable rice resistance to rice blast.

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Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight

Cited by 44 publications

References 26 publications

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Marker-assisted breeding of the rice restorer line Wanhui 6725 for disease resistance, submergence tolerance and aromatic fragrance

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

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