Marker-assisted selection in breeding silkworm strains with high silk production and resistance to the densonucleosis virus

Hou, Chengxiang; Sun, Ping-jiang; Guo, Xijie; Huang, Yongping; Li, M W

doi:10.4238/2013.october.7.3

Cited by 7 publications

(5 citation statements)

References 16 publications

(12 reference statements)

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“…Identification of SSR markers linked to genes was carried out in order to understand the molecular basis of characteristics of agronomic and scientific interest. The SSR loci identified by Miao et al 15 and Zhan et al 20 enabled the identification of genes related to characteristics such as thermotolerance, resistance to the Z strain of the virus of densonosis in B. mori, tolerance to fluorinated compounds, and absence of wing scales; studies that were used to develop improved lines with marker-assisted selection 9,65,66 . Genes related to pigmentation patterns were also identified, in cocoons and larvae, and in development processes such as the formation of extremities, thoracic segments, cell adhesion and regulation of molting (Table 3).…”

Section: Identification Of Genes and Qtlsmentioning

confidence: 99%

Role of microsatellites in genetic analysis of Bombyx mori silkworm: a review

et al. 2019

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In the genome of Bombyx mori Linnaeus (1758), the microsatellites, or simple sequence repeats (SSR), feature among their particular characteristics a high adenine and thymine (A/T) content, low number of repeats, low frequency, and a grouping in "families" with similar flanking regions. Such characteristics may be the result of a complex interaction between factors that limit the size and dispersion of SSR loci—such as their high association with transposons—and mean that microsatellites within this taxon suitable as molecular markers are relatively rare. The determination of genetic profiles in populations and cell lines has not been affected owing to the high level of polymorphism, nor has the analysis of diversity, structure and genetic relationships. However, the scarcity of suitable microsatellites has restricted their application in genetic mapping, limiting them to preliminary identification of gene location of genes or quantitative trait loci (QTLs) related to thermotolerance, resistance to viruses, pigmentation patterns, body development and the weight of the cocoon, the cortex, the pupa and the filament. The review confirms that, as markers, microsatellites are versatile and perform well. They could thus be useful both to advance research in emerging countries with few resources seeking to promote sericulture in their territories, and to advance in the genetic and molecular knowledge of characteristics of productive and biological interest, given the latest technological developments in terms of the sequencing, identification, isolation and genotyping of SSR loci.

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Section: Identification Of Genes and Qtlsmentioning

confidence: 99%

Role of microsatellites in genetic analysis of Bombyx mori silkworm: a review

et al. 2019

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“…From many years ago till now, many studies focused on mulberry varieties and their effects on economic traits and growth of silkworms, on the other hand, many efforts had been done by genetic breeding to find out a diseases resistance hybrids of B. mori (Alipanah et al, 2020;Hema et al, 2011;Hou et al, 2013;Kumar and Naik, 2011). But no investigations were done on the effect of mulberry varieties on immune response or disease tolerance.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Studies on the Humoral Immune Response of Mulberry Silkworm, Bombyx mori Larvae Fed on Different Mulberry Varieties

Mahmoud

2020

Journal of Applied Plant Protection

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Diseases infecting silkworms cause 30-40% cocoon crop loss. The silkworm, Bombyx mori L. defense against pathogens is provided by open circulatory system through humoral and cellular response. Three mulberry varieties, Morus alba var. Rosa, M. alba var. Morittiana and M. alba var. Laevigata, were used to study the effect of host plant and bacterial contagious infection on the humoral immune of B. mori larvae. The specific activity of related enzymes in haemolymph of fifth instar larvae was estimated. Results indicated that mulberry varieties and bacterial contagious infection had significant effects on enzymes activity values in larval haemolymph. Moreover, M. alba var. laevigata was superior in the humoral immune response.

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“…The silk yield of JS is about five-fold higher than that of L10. The silk yield is about 0.5 g per individual in Js, while that of L10 is about 0.1 g. Because cocoon and silk yields are determined by genetic quantitative traits, molecular marker techniques have been used widely in silkworms in order to identify many quantitative trait loci (QTLs) [ 5 – 7 ]; indeed, our laboratory has used such techniques to identify 14 QTLs [ 4 ]. Although technopositioning can be useful for finding the genetic loci of large fragments having relatively high location-dependent (LOD) values, RNA-Seq based transcriptome analysis may be more suitable for detecting minor genes [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Different Silk Yields of Two Silkworm Strains

Qin

et al. 2016

PLoS ONE

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Cocoon and silk yields are the most important characteristics of sericulture. However, few studies have examined the genes that modulate these features. Further studies of these genes will be useful for improving the products of sericulture. JingSong (JS) and Lan10 (L10) are two strains having significantly different cocoon and silk yields. In the current study, RNA-Seq and quantitative polymerase chain reaction (qPCR) were performed on both strains in order to determine divergence of the silk gland, which controls silk biosynthesis in silkworms. Compared with L10, JS had 1375 differentially expressed genes (DEGs; 738 up-regulated genes and 673 down-regulated genes). Nine enriched gene ontology (GO) terms were identified by GO enrichment analysis based on these DEGs. KEGG enrichment analysis results showed that the DEGs were enriched in three pathways, which were mainly associated with the processing and biosynthesis of proteins. The representative genes in the enrichment pathways and ten significant DEGs were further verified by qPCR, the results of which were consistent with the RNA-Seq data. Our study has revealed differences in silk glands between the two silkworm strains and provides a perspective for understanding the molecular mechanisms determining silk yield.

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Marker-assisted selection in breeding silkworm strains with high silk production and resistance to the densonucleosis virus

Cited by 7 publications

References 16 publications

Role of microsatellites in genetic analysis of Bombyx mori silkworm: a review

Role of microsatellites in genetic analysis of Bombyx mori silkworm: a review

Preliminary Studies on the Humoral Immune Response of Mulberry Silkworm, Bombyx mori Larvae Fed on Different Mulberry Varieties

Comparative Transcriptome Analysis Reveals Different Silk Yields of Two Silkworm Strains

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