Marker-assisted selection for white mold resistance in common bean

Ender, Márcio; Terpstra, Karolyn; Kelly, James D.

doi:10.1007/s11032-007-9115-9

Cited by 109 publications

(37 citation statements)

References 24 publications

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“…Miklas (2007) used molecular-marker-assisted selection to introgress WM-resistant QTL located on chromosome 7 in G 122 and 8 in NY6020-4. Similarly, Ender et al (2008) used marker-assisted selection for WM-resistance QTL located on chromosomes 2 and 7 in two common bean populations involving ICA Bunsi. Furthermore, they were able to enhance WM resistance by including seed yield and plant architectural traits in the selection process.…”

Section: Introductionmentioning

confidence: 99%

Gamete selection for improving physiological resistance to white mold in common bean

Terán

Singh

2009

Euphytica

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White mold (WM), caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a widespread disease of dry and green bean (Phaseolus vulgaris L.) in North America. Gamete selection (GS) was effective to combine and pyramide resistant genes and quantitative trait loci (QTL) for common bacterial blight. Our objective was to determine the effectiveness of GS to introgress physiological resistance to white mold. Two inter-gene-pool double-cross populations were developed. Selection for WM resistance was practiced from F 1 to F 4 . Thirteen selected F 1:5 breeding lines of each population and their four parents were evaluated. Two separate inoculations were made on each plant 1 week apart using a cut-stem method. The WM reaction was scored at 16, 23, and 33 days post inoculation (DPI) using a scale from 1 (no disease) to 9 (severely diseased or dead). In F 1 , 52% of Pop I (USPT-WM-1/CORN 601//USPT-CBB-1/92BG-7) and 67% of Pop II (Chase/I9365-25//ABL 15/A 195) susceptible plants were discarded. In F 4, only 1.2% of families from Pop I, and 0.9% for Pop II, survived the selection process. An average of 20.5% gain in WM resistance was obtained for both populations in F 4 . Four breeding lines of Pop I had significantly (P = 0.05) lower WM score (4.

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Section: Introductionmentioning

confidence: 99%

Gamete selection for improving physiological resistance to white mold in common bean

Terán

Singh

2009

Euphytica

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“…Molecular markers were also useful for introgression of gene(s) from indica into japonica variety of rice, so that new rice cultivars with combination of desirable traits from both rice varieties were developed (Rahman et al, 2008). Using molecular markers, an increased selection gain and a decreased selection cost were achieved in a snap bean breeding program to develop a cultivar resistant to white fungus diseases (Ender et al, 2008). In bell pepper, molecular markers were successfully employed in introgression of genes for resistance to Phytophtora (Thabuis et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Dna Marker-Assisted and Morphological Selection on Bc3 Genotypes Shortcut the Introgression of CMV Tolerance Genes on Chili Pepper

Herison¹,

Winarsih²,

Handayaningsih³

et al. 2012

Agrivita.J.Agr.Sci

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Superior hybrid and CMV tolerant cultivar development requires a pair of highly heterobeltiosis parents and both of which are CMV tolerant. Gene introgression has to be accomplished if the tolerance does not exist in the parents. The objective of this research was to employ DNA markers and morphological traits to identify CMV tolerant individuals in BC3 that were the most similar to their recurrent parents in order to shortcut the backcrossing cycle in chili pepper (Capsicum annuum L.). This research used fifteen BC3 populations generated from crosses between PBC1354 and PBC378 hybrid parents and tolerant parents of C1024, C1042 and C1043. The BC3 populations were previously selected for their CMV tolerance and were characterized by RAPD technique as well as morphological traits. Selection by means of both RAPD markers and morphological traits identified BC3 individuals with 99.9% similarity to their respective recurrent parent. B3A24-20, B3A29-13, B3A29-22, B3B12-13, B3B37-9, B3B12-25, B3C16-5, B3C16-16, B3C34-18 genotypes were 99.9% similar to PBC378; meanwhile, B3D11-8, B3D11-17, B3D38-5, B3E12-17, B3E20-22, B3E31-19 were 99.9% similar to PBC1354. Those genotypes were both similar to their recurrent parents and tolerant to CMV. The employed strategy shortened CMV tolerance gene introgression through backcross breeding.

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“…The indirect methods used pathogen filtrate to detect physiological resistance (Miklas et al 1992b), oxalic acid diffusion test (Tu 1985), modified oxalate test (Kolkman and Kelly 2000), and soluble stem pigment production in oxalic acid (Wegulo et al 1998). In addition, researchers used resistance-linked molecular markers to detect presence or absence of WM resistance quantitative trait loci (QTL) Kolkman and Kelly 2003;Ender and Kelly 2005;Maxwell et al 2007;Ender et al 2008).…”

Section: Mots Clé Smentioning

confidence: 99%

Efficacy of three greenhouse screening methods for the identification of physiological resistance to white mold in dry bean

Terán

Singh²

2009

Can. J. Plant Sci.

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Tera´n, H. and Singh, S. P. 2009. Efficacy of three greenhouse screening methods for the identification of physiological resistance to white mold in dry bean. Can. J. Plant Sci. 89: 755Á762. White mold (WM) caused by Sclerotinia sclerotiorum (Lib.) de Bary is the most devastating disease of common bean (dry and snap or garden bean) (Phaseolus vulgaris L.) in North America. The use of a reliable screening method (SM) in common bean is crucial to improve physiological resistance to WM. The objective of this study was to compare the efficacy of three SM to identify physiological resistance in dry bean genotypes with different evolutionary origins and levels of resistance. Screening methods tested were: (i) the modified straw test or cutÁstem (CSM); (ii) infected bean flower (IFL); and (iii) infected oat seed (IOS). A 195, ICA Bunsi, Othello, and VCW 54 dry bean were tested with the three SM. The experimental design was a split plot in randomized complete blocks with three replications in 2007 and 2008. Two independent inoculations 1 wk apart for each SM were made. The WM reaction was scored at 16, 23, and 33 d post-inoculation (DPI) using a 1 to 9 scale. There were highly significant differences between SM and its interaction with years. The CSM and IFL were the most consistent and highly correlated (r 0.70, P B0.01). Interspecific breeding line VCW 54 consistently had the highest WM resistance across years, SM, and evaluation dates, followed by A 195. White mold scores increased with delayed evaluations. Thus, CSM or IFL with disease assessed 33 DPI should be used for identifying common bean genotypes with high levels of physiological resistance to WM. . La ligne´e interspe´cifique VCW 54 a toujours pre´sente´la meilleure re´sistance a`la MB peu importe l'anne´e, la me´thode de pre´se´lection et la date d'e´valuation. Elle e´tait suivie par le cultivar A 195. Le degre´d'infection s'accroıˆt quand on retarde l'e´valuation. Les auteurs pre´conisent la me´thode CSM ou IFL avec e´valuation de la maladie 33 jours apre`s l'inoculation pour identifier les ge´notypes de haricot pre´sentant la meilleure re´sistance physiologique a`la MB.

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Marker-assisted selection for white mold resistance in common bean

Cited by 109 publications

References 24 publications

Gamete selection for improving physiological resistance to white mold in common bean

Gamete selection for improving physiological resistance to white mold in common bean

Dna Marker-Assisted and Morphological Selection on Bc3 Genotypes Shortcut the Introgression of CMV Tolerance Genes on Chili Pepper

Efficacy of three greenhouse screening methods for the identification of physiological resistance to white mold in dry bean

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