Marker-assisted selection for the wide-spectrum resistance to root-knot nematodes conferred by the Ma gene from Myrobalan plum (Prunus cerasifera) in interspecific Prunus material

Lecouls, A.C.; Bergougnoux, Véronique; Rubio-Cabetas, M. J.; Bosselut, Nathalie; Voisin, R; Poëssel, Jean‐Luc; Faurobert, Mireille; Bonnet, A.; Salesses, G.; Dirlewanger, Elisabeth; Esmenjaud, Daniel

doi:10.1023/b:molb.0000018758.56413.cf

Cited by 47 publications

(36 citation statements)

References 22 publications

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“…The digested products were then separated on a 2% agarose gel and stained with ethidium bromide. The SCAL19 and SCAFLP2 markers were amplified as described in Lecouls et al (2004).…”

Section: Pcr Experimentsmentioning

confidence: 99%

High-resolution mapping and chromosome landing at the root-knot nematode resistance locus Ma from Myrobalan plum using a large-insert BAC DNA library

Claverie¹,

Dirlewanger

Cosson

et al. 2004

Theor Appl Genet

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The Ma gene for root-knot nematode (RKN)resistance from Myrobalan plum (Prunus cerasifera L.)confers a complete-spectrum and a heat-stable resistance to Meloidogvne spp., conversely to Mi-I from tomato,which has a more restricted spectrum and a reduced efficiency at high temperature. This gene was identified from a perennial self-incompatible near-wild rootstock species and lies in cosegregation with the SCAR marker SCAFLP2 on the Prunus linkage group 7 in a 2.3 cM interval between the SCAR SCAL19 and SSR pchgms6 markers. We initiated a map-based cloning of Ma and report here the strategy that rapidly led to fine mapping and direct chromosome landing at the locus. Three pairs of bulks, totaling 90 individuals from half-sibling progenies derived from the Ma-heterozygous resistant accession P.2175, were constructed using mapping data, and saturation of the Ma region was performed by bulked segregant analysis (BSA) of 320 AFLP primer pair combinations. The closest three AFLP markers were transformed into codominant SCARs or CAPS designatedSCAFLP3, SCAFLP4 and SCAFLP5. By completing the mapping population up to 1,332 offspring from P.2175,Ma and SCAFLP2 were mapped in a 0.8 cM interval between SCAFLP3 and SCAFLP4. A large-insert bacterial artificial chromosome (BAC) DNA library of P.2175,totaling 30,720 clones with a mean insert size of 145 kb and a 14-15x Prunus haploid genome coverage was constructed and used to land on the Ma spanning interval with few BAC clones. As P.2175 is heterozygous for the gene, we constructed the resistant and susceptible physical contigs by PCR screening of the library with codominant markers. Additional microsatellite markers were then designed from BAC subcloning or BAC end sequencing. In the resistant contig, a single 280 kb BAC clone was shown to carry the Ma gene; this BAC contains two flanking markers on each side of the gene as well as two cosegregating markers. These results should allow future cloning of the Ma gene in this perennial species.

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“…The digested products were then separated on a 2% agarose gel and stained with ethidium bromide. The SCAL19 and SCAFLP2 markers were amplified as described in Lecouls et al (2004).…”

Section: Pcr Experimentsmentioning

confidence: 99%

High-resolution mapping and chromosome landing at the root-knot nematode resistance locus Ma from Myrobalan plum using a large-insert BAC DNA library

Claverie¹,

Dirlewanger

Cosson

et al. 2004

Theor Appl Genet

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“…Until now, RKN have been mainly controlled by preplantation fumigations with methyl bromide (that will be completely removed for Prunus species by the end of 2004) or other highly toxic fumigants. The search for RKN resistant Prunus rootstocks as an alternative control method has been a long and continuous task in many countries (Minz and Cohn 1962;Sharpe et al 1969;Kochba and Spiegel-Roy 1975;Sherman et al 1981;Ramming and Tanner 1983;Kester and Grassely 1987;Lu et al 1999;Yamamoto et al 2001;Lecouls et al 2004). The efficiency of RKN resistance in rootstocks depends on the source of resistance (Scotto La Massese et al 1984;Esmenjaud et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid?location of root-knot nematode resistance genes

et al. 2004

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Inheritance and linkage studies were carried out with microsatellite [or simple sequence repeat (SSR)] markers in a F(1) progeny including 101 individuals of a cross between Myrobalan plum ( Prunus cerasifera Ehrh) clone P.2175 and the almond (Prunus dulcis Mill.)-peach ( Prunus persica L. Batsch) hybrid clone GN22 ["Garfi" (G) almond x "Nemared" (N) peach]. This three-way interspecific Prunus progeny was produced in order to associate high root-knot nematode (RKN) resistances from Myrobalan and peach with other favorable traits for Prunus rootstocks from plum, peach and almond. The RKN resistance genes, Ma from the Myrobalan plum clone P.2175 and R(MiaNem) from the 'N' peach, are each heterozygous in the parents P.2175 and GN22, respectively. Two hundred and seventy seven Prunus SSRs were tested for their polymorphism. One genetic map was constructed for each parent according to the "double pseudo-testcross" analysis model. The Ma gene and 93 markers [two sequence characterized amplified regions (SCARs), 91 SSRs] were placed on the P.2175 Myrobalan map covering 524.8 cM. The R(MiaNem) gene, the Gr gene controlling the color of peach leaves, and 166 markers (one SCAR, 165 SSRs) were mapped to seven linkage groups instead of the expected eight in Prunus. Markers belonging to groups 6 and 8 in previous maps formed a single group in the GN22 map. A reciprocal translocation, already reported in a G x N F(2), was detected near the Gr gene. By separating markers from linkage groups 6 and 8 from the GN22 map, it was possible to compare the eight homologous linkage groups between the two maps using the 68 SSR markers heterozygous in both parents (anchor loci). All but one of these 68 anchor markers are in the same order in the Myrobalan plum map and in the almond-peach map, as expected from the high level of synteny within Prunus. The Ma and R(MiaNem)genes confirmed their previous location in the Myrobalan linkage group 7 and in the GN22 linkage group 2, respectively. Using a GN22 F(2) progeny of 78 individuals, a microsatellite map of linkage group 2 was also constructed and provided additional evidence for the telomeric position of R(MiaNem) in group 2 of the Prunus genome.

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“…Three PCR-based genetic markers linked to Ma, SCAFLP3 (proximal; 10 recombinants with the Ma gene), SCAFLP2 (cosegregating; Lecouls et al, 2004), and SCAFLP4 (distal; a single recombinant; Supplemental Table S1), among 1,332 meioses (Claverie et al, 2004b), were placed on the sequence and determined a physical interval of 174 kb containing Ma. New simple sequence repeat (SSR) markers (Supplemental Table S1) were manually designed within this 174-kb interval and mapped on the same recombinant progeny, allowing us to reduce the interval containing the Ma gene to 70 kb flanked by the markers SSR2 and SCAFLP4 (Fig.…”

Section: Detection Of Candidate Genes Spanning the Ma Locusmentioning

confidence: 99%

TheMaGene for Complete-Spectrum Resistance toMeloidogyneSpecies inPrunusIs a TNL with a Huge Repeated C-Terminal Post-LRR Region

et al. 2011

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Root-knot nematode (RKN) Meloidogyne species are major polyphagous pests of most crops worldwide, and cultivars with durable resistance are urgently needed because of nematicide bans. The Ma gene from the Myrobalan plum (Prunus cerasifera) confers complete-spectrum, heat-stable, and high-level resistance to RKN, which is remarkable in comparison with the Mi-1 gene from tomato (Solanum lycopersicum), the sole RKN resistance gene cloned. We report here the positional cloning and the functional validation of the Ma locus present at the heterozygous state in the P.2175 accession. High-resolution mapping totaling over 3,000 segregants reduced the Ma locus interval to a 32-kb cluster of three Toll/Interleukin1 Receptor-Nucleotide Binding Site-Leucine-Rich Repeat (LRR) genes (TNL1-TNL3), including a pseudogene (TNL2) and a truncated gene (TNL3). The sole complete gene in this interval (TNL1) was validated as Ma, as it conferred the same complete-spectrum and high-level resistance (as in P.2175) using its genomic sequence and native promoter region in Agrobacterium rhizogenes-transformed hairy roots and composite plants. The full-length cDNA (2,048 amino acids) of Ma is the longest of all Resistance genes cloned to date. Its TNL structure is completed by a huge post-LRR (PL) sequence (1,088 amino acids) comprising five repeated carboxylterminal PL exons with two conserved motifs. The amino-terminal region (213 amino acids) of the LRR exon is conserved between alleles and contrasts with the high interallelic polymorphisms of its distal region (111 amino acids) and of PL domains. The Ma gene highlights the importance of these uncharacterized PL domains, which may be involved in pathogen recognition through the decoy hypothesis or in nuclear signaling.

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Marker-assisted selection for the wide-spectrum resistance to root-knot nematodes conferred by the Ma gene from Myrobalan plum (Prunus cerasifera) in interspecific Prunus material

Cited by 47 publications

References 22 publications

High-resolution mapping and chromosome landing at the root-knot nematode resistance locus Ma from Myrobalan plum using a large-insert BAC DNA library

High-resolution mapping and chromosome landing at the root-knot nematode resistance locus Ma from Myrobalan plum using a large-insert BAC DNA library

Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid?location of root-knot nematode resistance genes

TheMaGene for Complete-Spectrum Resistance toMeloidogyneSpecies inPrunusIs a TNL with a Huge Repeated C-Terminal Post-LRR Region

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