Marker-assisted selection and gene pyramiding for resistance to bacterial leaf blight disease of rice (<i>Oryza sativa</i>L.)

Chukwu, Samuel Chibuike; Rafii, Mohd Y.; Ramlee, Shairul Izan; Ismail, Siti Izera; Oladosu, Yusuff; Okporie, Emmanuel Ogbonna; Onyishi, G. C.; Utobo, E. B.; Ekwu, L. G.; Swaray, Senesie; Jalloh, Momodu

doi:10.1080/13102818.2019.1584054

Cited by 63 publications

(41 citation statements)

References 118 publications

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“…The result confirms the successful pyramiding of the target genes into the BC 2 F 1 populations using marker-assisted foreground selection. Foreground markers are closely associated or linked to the gene of interest on the same chromosome and as such are carried from one generation to another [22,23].…”

Section: Marker Genotyping Of Bc 2 F 1 Populationmentioning

confidence: 99%

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

et al. 2019

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Bacterial leaf blight caused by Xanthomonas oryzae pv oryzae (Xoo) and blast caused by Magnaporthe oryzae are major diseases responsible for significant yield loss in rice production across all rice growing regions. Host plant resistance has been advocated as a sustainable means of guarding against the diseases. This experiment was conducted with the aim to introgress multiple resistance genes against bacterial leaf blight and blast diseases through marker-assisted backcross breeding. Two dominant (Xa4 and Xa21) and two recessive (xa5 and xa13) Xoo resistance genes were introgressed into a high yielding Malaysian rice variety Putra-1 with genetic background of three blast resistance (Piz, Pi2 and Pi9) genes. Eight polymorphic tightly linked functional and SSR markers were used for foreground selection of target genes. Seventy nine polymorphic SSR markers were used in background selection. The plants were challenged at initial stage of breeding and challenged again at BC2F2 with the most virulent Malaysian pathotypes of Xoo (P7.7) and Magnaporthe oryzae (P7.2) to test their resistance. Results obtained from foreground marker segregation analysis at BC1F1 and BC2F1 showed that the marker polymorphism both fitted into the Mendel’s single gene segregation ratio of 1:1 for both Xoo and blast resistance. At BC2F2, results indicated that foreground marker polymorphism fitted into the expected Mendelian ratio of 1:2:1 for blast resistance only. Marker-assisted background selection revealed high percentage of recurrent parent genome recovery (95.9%). It was concluded that the inheritance of blast resistance in the introgressed lines was mainly due to single gene action while the inheritance of Xoo resistance was substantially due to single nuclear gene action. The incorporation of four bacterial leaf blight and three blast resistance genes (Xa4 + xa5 + xa13 + Xa21; Pi9 + Pi2 + Piz) in the newly developed lines would provide for broad spectrum and durable resistance against the two major diseases studied.

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Section: Marker Genotyping Of Bc 2 F 1 Populationmentioning

confidence: 99%

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

et al. 2019

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Section: Confirmation Using Polymorphic Tightly Linked Foreground Marmentioning

confidence: 99%

Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in Putra-1 rice variety

Rafii

Chukwu

Ramlee

et al. 2019

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This experiment was conducted with the aim of introgressing multiple resistance genes against bacterial leaf blight (BLB) and blast diseases through marker-assisted backcross breeding. Two dominant (Xa4 and Xa21) and two recessive (xa5 and xa13) BLB resistance genes were introgressed into a Malaysian elite rice variety Putra-1 with genetic background of three blast resistance (Piz, Pi2 and Pi9) genes and high yielding. Eight polymorphic tightly linked functional and SSR markers were used for foreground selection of target genes. 79 polymorphic SSR markers were used in background selection. The plants were challenged at initial stage of breeding and challenged again at BC 2 F 2 with the most virulent Malaysian pathotypes of Xoo (P7.7) and Magnaporthe oryzae (P7.2) to test their resistance. Results obtained from foreground marker analysis showed that the BC 1 F 1 and BC 2 F 1 both fitted into the Mendel's single gene segregation ratio of 1:1 for both Xoo and blast resistance. At BC 2 F 2 , result obtained indicated that foreground marker segregation fitted into the expected Mendelian ratio of 1:2:1 for blast resistance only. Marker-assisted background selection revealed high percentage of recurrent parent genome recovery (95.9%). It was concluded that resistance to Xoo pathotype P7.7 in IRBB60 was neither due to two independent gene action nor epistasis but substantially due to single nuclear gene action. Also, the inheritance of blast resistance in the pyramided lines to pathotype P7.2 was also attributed to single gene action. The incorporation of four bacterial leaf blight and three blast resistance genes (Xa4+xa5+xa13+Xa21+Pi9+Pi2+Piz) in the newly developed lines provides for broad spectrum and durable resistance against the two major diseases studied.

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“…However, this disease occurs due to the interaction of a favorable environment, a susceptible genotype and a virulent pathogen. Hence, the utilization of resistant cultivar is a favorable way to reduce the use of destructive pesticides [15,16]. Molecular markers have been intensively used to pyramid beneficial and multiple alleles to develop new rice blast resistant varieties [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Agro-Morphologic Performance, Genetic Parameters and Clustering Pattern of Newly Developed Blast Resistant Rice Lines Tested in Four Environments

et al. 2020

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Multi-environmental yield trial is very vital in assessing newly developed rice lines for its adaptability and stability across environments especially prior to release of the newly developed variety for commercial cultivation. The growth performance and phenotypic variability of these genotypes are the combination of environment, genotype and genotype by environment (G×E) interaction factors. Thus, evaluation creates an opportunity for effective selection of superior genotypes. The objectives of this study were to evaluate the newly developed blast resistant rice lines in varied environmental conditions, precisely measure the response of the advanced lines in multiple environments and classify the genotypes into groups that could serve as varieties for commercial cultivation. Genetic materials included 18 improved blast resistant rice lines and the recipient parent MR219. The total of 19 newly developed genotypes was evaluated under four varied environments in Peninsular Malaysia. The experiments were carried out using randomized complete block design (RCBD) with three replications at each environment. Data were collected on the vegetative, yield and yield component traits. Descriptive statistics (mean performance) and analysis of variance were conducted using SAS Software version 9.4. Genotypic and phenotypic coefficients, phenotypic variance component, heritability and genetic advance were also determined. Analysis of variance revealed that all traits were significantly different for genotypes except days to maturity, number of filled grains and total number of grains. Meanwhile, all the traits differed significantly for genotype × environment (G×E) except number of tillers per hill and number of panicles per hill. Low heritability (<30%) was found for all the traits. Similarly, low genetic advance was also observed for all the traits except for number of tillers per hill and number of panicles per hill. yield per hectare had significant and positive correlation with most evaluated traits except for days to flowering, days to maturity, plant height and number of unfilled grains. Cluster analysis classified the 19 evaluated genotypes into six groups. Therefore, the six clusters/groups of genotypes were recommended as varieties for commercial cultivation in Malaysia and other rice growing regions.

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Marker-assisted selection and gene pyramiding for resistance to bacterial leaf blight disease of rice (Oryza sativaL.)

Cited by 63 publications

References 118 publications

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in Putra-1 rice variety

Assessment of Agro-Morphologic Performance, Genetic Parameters and Clustering Pattern of Newly Developed Blast Resistant Rice Lines Tested in Four Environments

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