Marker-assisted pyramiding of major blast resistance genes in Swarna-Sub1, an elite rice variety (Oryza sativa L.)

Patroti, Parashuram; Vishalakshi, B.; Umakanth, B.; Suresh, J.; Senguttuvel, P.; Madhav, M. S.

doi:10.1007/s10681-019-2487-1

Cited by 13 publications

(10 citation statements)

References 35 publications

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“…A higher recovery of desirable improved plants of the pyramided lines BC 2 F 2:3-7-33 and BC 2 F 2:3-7-16 was obtained because of phenotypic-based selection in each backcross generation. The current strategies of phenotypic and marker-based selection are consistent with the findings of Joseph et al ( 2004 ), Gopalakrishnan et al ( 2008 ), Tanweer et al ( 2015 ), and Patroti et al ( 2019 ), who adopted the phenotypic and marker-assisted selection for the target traits. In this study, the phenotypic-based selection process was adopted from the BC 1 F 1 generation onward for the agro-morphological traits while screening a large number of backcross plants.…”

Section: Discussionsupporting

confidence: 81%

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

et al. 2021

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Cauliflower is an important extensively grown cool season vegetable in India. Black rot and downy mildew are major devastating diseases reducing yield and quality of the crop. To tackle these through host plant resistance, a marker-assisted backcross breeding method was followed to pyramid a black rot-resistant gene (Xca1bo) and a downy mildew-resistant gene (Ppa3) from donors BR-161 and BR-2, respectively, into the background of Pusa Meghna cauliflower cultivar. Marker-assisted backcross breeding was followed up to BC2 generation using SCAR marker ScOPO-04833 and SSR marker BoGMS0624 for black rot and downy mildew resistance genes in foreground selection, respectively. In background selection, at each stage of backcrossing, 47 parental polymorphic SSR markers were used. The graphical genotyping of the five two-gene (Xca1boXca1boPpa3Ppa3) homozygous BC2F2 plants showed an average recovery of 85.44% of the Pusa Meghna genome with highest genome recovery of 91.7%. The genome contribution of donor parents (BR-161 and BR-2) was 8.26 with 6.34% of residual heterozygousity. The backcross derived pyramided lines BC2F2:3-7-16 and BC2F2:3-7-33 showed high resistance to both the diseases and exhibited higher yield and vitamin C content as compared with recipient parent Pusa Meghna. It is, therefore, evident from this study that resistant genes can be introgressed successfully into a Pusa Meghna cultivar without any yield penalty, benefitting farmers with reduced input cost and consumers with chemical residue free produce. Besides, the pyramided lines carrying dominant resistant genes can be exploited in a hybridization programme to develop hybrid(s) in cauliflower.

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Section: Discussionsupporting

confidence: 81%

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

et al. 2021

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“…The present study demonstrates successful incorporation of genetic resistance to these diseases using MABC in a popular cold tolerant, long slender grain rice variety Tellahamsa. Marker assisted selection is a widely adapted technique which is being used by several researchers to introgress the genes and it becomes more successful when the markers perfectly linked to the trait of interest are available (JGL1798, Jin 23B hybrids and Swarna Sub1) [35][36][37]. Although there are different resistance sources available for BB, we used ISM (Improved Samba Mahsuri which has Xa21 xa13 and xa5) as donor, since it has good cooking quality and…”

Section: Discussionmentioning

confidence: 99%

“…In Indian climatic conditions Pi54, Pi1 and Pi2 offer significant level of resistance to blast across environments [40,41]. The combinations of Pi54 and Pi1 in case of Samba Mahsuri, Swarna, Swarna sub-1 and Pusa Basmati 1 (PB 1) have showed high level of blast resistance [42][43][44][45]. Recently, many research workers have reported a highly effective blast resistance of gene pyramided lines possessing Pi54 and Pi2 in diverse locations of India [46,43,38].…”

Section: Plos Onementioning

confidence: 99%

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

et al. 2020

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Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a welladapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F 1 to ICF 3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC 2 F 1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1-ICF 3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF 3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.

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“…The genetic dissection of the offspring with closely linked molecular markers for the gene of interest is an effective way of speeding up the selection process at each generation for developing the superior varieties with maximum RPG recovery [3]. Patroti et al [7,20] used same markers (RM206, PI54 MAS and RM224) for the foreground selection of Pi54 and Pi1 genes in to the genetic background of Swarna Sub1 and Improved Samba Mahsuri. Swathi et al [8] carried out foreground selection of blast resistant gene, Pi54 using functional marker Pi54 MAS in the genetic background JGL1798.…”

Section: Resultsmentioning

confidence: 99%

“…Till date, more than 100 rice blast resistance genes and 340 QTLs were identified [6]. Among them, two major blast resistance genes viz.,Pi1 and Pi54 located on chromosome 11 are known to be very effective which were being used in introgression into several genetic backgrounds [7,8,9]. Therefore, we have chosen to validate the markers linked to qDTY 12.1 for improvising yield under drought stress and Pi1 and Pi54 for blast resistance.…”

Section: Introductionmentioning

confidence: 99%

Validation of Markers Linked to Yield under Drought, Blast Resistance and Assessment of Polymorphism among the Donors for Use in Marker Assisted Selection in Rice

Vishalakshi

Umakanth

Usha

et al. 2020

CJAST

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Rice is one of the most widely cultivated crop species in the world. Drought, a major constraint in upland rice (Oryza sativa L.) led to the unstable yields. The inconsistent yields often aggravated by the severe incidence of blast disease in many areas of rainfed ecosystem. To improve upland rice, introgression of two traits viz., yield under drought stress and blast resistance is highly essential. We validated the markers linked to the qDTY12.1 (exhibits grain yield under drought stress) and two important blast resistance genes (Pi1 and Pi54) among the 12 important rice genotypes to use these markers in the foreground selection. Parental polymorphic survey was also conducted among the donor for the yield under drought (Vandana NIL possessing qDTY12.1), upland rice variety Varalu and donor for blast resistance (line possessing Pi1 and Pi54 genes) using 500 SSR markers distributed across the genome. The polymorphism between Varalu, Vandana NIL is 30% whereas it is 24% between Varalu, blast donor and around 16% between three parents. The identified polymorphic markers which are linked to the genes as well as the parental polymorphic markers are useful to carry out foreground and background selection in marker assisted selection programme. In addition, the markers lies proximal and distal ends of the target genes from the respective chromosomes were also identified which can be used in recombinant selection. Thus the results emanated from this investigation are useful for the combining yield under drought and blast resistance traits through molecular breeding programme for development of rice variety suitable to the rainfed ecosystem.

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Marker-assisted pyramiding of major blast resistance genes in Swarna-Sub1, an elite rice variety (Oryza sativa L.)

Cited by 13 publications

References 35 publications

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

Validation of Markers Linked to Yield under Drought, Blast Resistance and Assessment of Polymorphism among the Donors for Use in Marker Assisted Selection in Rice

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