2021
DOI: 10.1038/s41598-021-92010-8
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Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids

Abstract: Malnutrition affects growth and development in humans and causes socio-economic losses. Normal maize is deficient in essential amino acids, lysine and tryptophan; and vitamin-A. Crop biofortification is a sustainable and economical approach to alleviate micronutrient malnutrition. We combined favorable alleles of crtRB1 and lcyE genes into opaque2 (o2)-based four inbreds viz. QLM11, QLM12, QLM13, and QLM14 using marker-assisted backcross breeding. These are parents of quality protein maize versions of two elit… Show more

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Cited by 19 publications
(11 citation statements)
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References 56 publications
(86 reference statements)
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“…This result was in agreement with the studies conducted by Visscher et al (1996) and Frisch et al (1999) [135,136]. In addition, Singh et al (2021) studied the introgression of favorable alleles of crtRB1 and lcyE genes into opaque2 (o2)-based four inbreds, namely, QLM11, QLM12, QLM13, and QLM14 using marker-assisted backcross breeding [137]. Awata et al (2021) used marker-assisted backcross (MABC) and doubled haploid (DH) methods to transfer genes for resistance to maize lethal necrosis (MLN).…”
Section: Recovery Of the Recurrent Parentsupporting
confidence: 92%
See 1 more Smart Citation
“…This result was in agreement with the studies conducted by Visscher et al (1996) and Frisch et al (1999) [135,136]. In addition, Singh et al (2021) studied the introgression of favorable alleles of crtRB1 and lcyE genes into opaque2 (o2)-based four inbreds, namely, QLM11, QLM12, QLM13, and QLM14 using marker-assisted backcross breeding [137]. Awata et al (2021) used marker-assisted backcross (MABC) and doubled haploid (DH) methods to transfer genes for resistance to maize lethal necrosis (MLN).…”
Section: Recovery Of the Recurrent Parentsupporting
confidence: 92%
“…In fact, as Liebermann [136] puts it, "many researchers [137] thus consider Homo sapiens to be a morphologically diverse species with archaic and anatomically modern grades." And, in his 2019 article, Caldararo argues that no amount of mathematical jiggling can demonstrate a clear separation between the Upper Paleolithic premodern populations and present-day humans.…”
Section: Gene Frequencies Speciation and Character Traitsmentioning
confidence: 99%
“…These heterotic groups (HGs) are composed of parental inbred lines that exhibit complementary genetic backgrounds, resulting in the expression of heterosis when crossed to opposite HGs (J. Singh et al, 2021). To develop these HGs, various methods have been employed in maize breeding programs.…”
Section: Introductionmentioning
confidence: 99%
“…Marker-assisted breeding with Meta-analysis is a powerful strategy for the identification of quantitative trait loci with a gene of interest for introgressive biofortification in maize because conventional breeding is a long-run approach, agronomic fortification like micro-organism-based inoculation to rich the desired level of nutrients from the beginning isn't a sustainable type, and transgenic fortification is more controversial and costly due to the diverse germplasm of maize [ 33 , 34 , 35 ]. Microorganism-based inoculum on seed or soil with techniques called 16S rRNA sequencing was used to identify Bacillus sp.…”
Section: Introductionmentioning
confidence: 99%