Marker assisted pyramiding of drought yield QTLs into a popular Malaysian rice cultivar, MR219

Abstract

BackgroundThree drought yield QTLs, qDTY2.2, qDTY3.1, and qDTY12.1 with consistent effect on grain yield under reproductive stage drought stress were pyramided through marker assisted breeding with the objective of improving the grain yield of the elite Malaysian rice cultivar MR219 under reproductive stage drought stress. Foreground selection using QTL specific markers, recombinant selection using flanking markers, and background selection were performed. BC1F3-derived lines with different combinations of qDTY2.2, qDTY3.1, and qDTY12.1 were evaluated under both reproductive stage drought stress and non-stress during the dry seasons of 2013 and 2014 at IRRI.ResultsThe grain yield reductions in the stress trials compared to non-stress trials ranged from 79 to 93 %. In the stress trials, delay in days to flowering and reduction in plant height were observed. In both seasons, MR219 did not produce any yield under stress, however it produced a yield of 5917 kg ha−1 during the 2013 dry season and 8319 kg ha−1 during the 2014 dry season under non-stress. Selected introgressed lines gave a yield advantage of 903 to 2500 kg ha−1 over MR219 under reproductive stage drought stress and a yield of more than 6900 kg ha−1 under non-stress during the 2014 dry season. Among lines with single qDTY, lines carrying qDTY2.2 provided a higher yield advantage under reproductive stage drought stress in the MR219 background. Two-qDTY combinations (qDTY3.1+qDTY2.2 and qDTY3.1+qDTY12.1) performed better than lines with three qDTY combinations, indicating the absence of positive interactions between the three qDTYs.ConclusionWe successfully developed drought-tolerant MR219 pyramided lines with a yield advantage of more than 1500 kg ha−1. Differential yield advantages of different combinations of the qDTYs indicate a differential synergistic relationship among qDTYs. This is the first report on the successful effect of qDTYs in increasing the yield under drought in genetic backgrounds other than those in which the qDTYs were earlier identified.

Identification of new QTL for salt tolerance from rice variety Pokkali

“…SNP markers and crop breeding chips are becoming the major marker system of choice for genetic mapping and marker‐assisted breeding in many crops (Chen et al, ; Rasheed et al, ; Thomson, ). Quantity trait locus pyramiding using molecular markers has been successfully used for developing superior genotypes with improved abiotic tolerance in rice breeding (Cheng et al, ; Endo et al, ; Shamsudin et al, ; Wenzl et al, ). In the current study, a customized Rice56K SNP array was applied in genotyping the RILs.…”

Penilaian Sifat Fisio-kimia dan Analisis Hedonik bagi Titisan Piramid Padi Tahan Kemarau Terpilih

“…Kajian oleh Shamsudin et al (2016aShamsudin et al ( , 2016b) berjaya menghasilkan beberapa PL dengan kelebihan hasil 0.5 tan sehektar atau lebih berbanding induk-induk penerima iaitu MR219 dan MRQ74 dalam tekanan kemarau di peringkat reproduktif dan tanpa kemarau melalui kaedah MAB langkah demi langkah. Tiga lokus ciri kuantitatif bagi hasil (qDTY) iaitu qDTY 2.2 , qDTY 3.1 dan qDTY 12.1 telah dipindahkan secara serentak ke dalam MR219 dan MRQ74 dan kehadiran kesemua qDTY pada PL disahkan menggunakan penanda mikrosatelit yang berkait rapat dengannya.…”

“…Penilaian dijalankan menggunakan reka bentuk rawak lengkap dengan tiga replikasi. PL yang dihasilkan mempunyai antara 83% hingga 98% dan antara 82% hingga 90% kandungan genom induk penerima bagi populasi MR219 dan MRQ74 dengan pemilihan dilakukan berdasarkan morfologi pokok dan bentuk bijian yang sama dengan induk penerima selain ketahanannya terhadap kemarau (Shamsudin et al 2016a(Shamsudin et al , 2016b. Kesemua genotip diambil daripada percubaan hasil lanjutan (AYT) pada musim utama 2016 di Stesen Padi, Tanaman Industri dan Florikultur (PTIF), Titi Serong, Perak.…”

Current Applicable DNA Markers for Marker Assisted Breeding in Abiotic and Biotic Stress Tolerance in Rice (Oryza sativa L.)

“…RM8085 was mapped on chromosome 1 at 139.9 cM and is linked to leaf rolling and leaf drying under drought stress (Salunkhe et al 2011). Shamsudin et al (2016) used three drought yield QTLs, qDTY 2.2, qDTY 3.1, and qDTY 12.1 with consistent effect on grain yield under reproductive stage drought stress in gene pyramiding work for an elite Malaysian rice cultivar. These three QTLs have effectively worked in foreground selection in each of their breeding generation.…”