Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection

Zampieri, Elisa; Volante, Andrea; Marè, Caterina; Orasen, G.; Desiderio, Francesca; Biselli, Chiara; Canella, Marco; Carmagnola, Lorena; Milazzo, Joëlle; Adreit, Henri; Tharreau, Didier; Poncelet, Nicolas; Vaccino, P.; Valè, Giampiero

doi:10.3390/plants12040757

Cited by 7 publications

(3 citation statements)

References 63 publications

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“…Evidence in the literature support that the introduction of distinct blast R genes into a susceptible variety through conventional breeding methods, either alone or in combinations, is effective to confer resistance against M. oryzae infection. They include Pi1, Pi2, Pi9, Pi20(t), Pi33, Pi39 and Pi40(t), Pi47, Pi48, Pi54, Pi56, PiZ, Pizt and Pig, among others [16,18,22,28,55]. In particular, the Pigm gene has been shown to be effective to control both leaf and panicle [56].…”

Section: Discussionmentioning

confidence: 99%

“…The production of blast-resistant japonica lines derived from japonica × indica crosses well adapted to temperate conditions remains challenging, not only because of the spectrum of resistance against M. oryzae races and the risk of resistance breakdown, but also because of the risk of the sterility of hybrids. Even so, the introgression of indica R genes (Piz, Pib, Pita and Pik) into a susceptible japonica variety has proven to be effective in conferring resistance against the blast population in Northern Italy [22].…”

Section: Introductionmentioning

confidence: 99%

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Development and Genome-Wide Analysis of a Blast-Resistant japonica Rice Variety

Escolà,

González-Miguel,

Campo

et al. 2023

Plants

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Rice is one of the most important crops in the world, and its production is severely affected by the rice blast disease caused by the fungus Magnaporthe oryzae. Several major blast resistance genes and QTLs associated with blast resistance have been described and mostly identified in indica rice varieties. In this work, we report the obtention of a blast-resistant rice breeding line derived from crosses between the resistant indica variety CT13432 and the japonica elite cultivar JSendra (highly susceptible to blast). The breeding line, named COPSEMAR9, was found to exhibit resistance to leaf blast and panicle blast, as demonstrated by disease assays under controlled and field conditions. Furthermore, a high-quality genome sequence of the blast-resistant breeding line was obtained using a strategy that combines short-read sequencing (Illumina sequencing) and long-read sequencing (Pacbio sequencing). The use of a whole-genome approach allowed the fine mapping of DNA regions of indica and japonica origin present in the COPSEMAR9 genome and the identification of parental gene regions potentially contributing to blast resistance in the breeding line. Rice blast resistance genes (including Pi33 derived from the resistant parent) and defense-related genes in the genome of COPSEMAR9 were identified. Whole-genome analyses also revealed the presence of microRNAs (miRNAs) with a known function in the rice response to M. oryzae infection in COPSEMAR9, which might also contribute to its phenotype of blast resistance. From this study, the genomic information and analysis methods provide valuable knowledge that will be useful in breeding programs for blast resistance in japonica rice cultivars.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Genome-Wide Analysis of a Blast-Resistant japonica Rice Variety

Escolà,

González-Miguel,

Campo

et al. 2023

Plants

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“…The gene combinations Pigm + Pi1 , Pigm + Pi54 , and Pigm + Pi33 displayed broad-spectrum resistance ( Wu et al., 2019 ). Broad-spectrum blast resistance was also achieved in the temperate japonica varieties by pyramiding three to four genes with Piz , Pib , Pik , Pita , and Pita2 ( Zampieri et al., 2023 ). As proven in many previous studies, stacking suitable blast genes/alleles has strong potential to obtain durable and broad-spectrum resistance in the breeding program.…”

Section: Fungal Diseases and Available Genetic Resourcesmentioning

confidence: 99%

Available cloned genes and markers for genetic improvement of biotic stress resistance in rice

Simon,

Hechanova,

Hernandez

et al. 2023

Front. Plant Sci.

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Biotic stress is one of the major threats to stable rice production. Climate change affects the shifting of pest outbreaks in time and space. Genetic improvement of biotic stress resistance in rice is a cost-effective and environment-friendly way to control diseases and pests compared to other methods such as chemical spraying. Fast deployment of the available and suitable genes/alleles in local elite varieties through marker-assisted selection (MAS) is crucial for stable high-yield rice production. In this review, we focused on consolidating all the available cloned genes/alleles conferring resistance against rice pathogens (virus, bacteria, and fungus) and insect pests, the corresponding donor materials, and the DNA markers linked to the identified genes. To date, 48 genes (independent loci) have been cloned for only major biotic stresses: seven genes for brown planthopper (BPH), 23 for blast, 13 for bacterial blight, and five for viruses. Physical locations of the 48 genes were graphically mapped on the 12 rice chromosomes so that breeders can easily find the locations of the target genes and distances among all the biotic stress resistance genes and any other target trait genes. For efficient use of the cloned genes, we collected all the publically available DNA markers (~500 markers) linked to the identified genes. In case of no available cloned genes yet for the other biotic stresses, we provided brief information such as donor germplasm, quantitative trait loci (QTLs), and the related papers. All the information described in this review can contribute to the fast genetic improvement of biotic stress resistance in rice for stable high-yield rice production.

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