Marker-assisted introgression of the major bacterial blight resistance gene, Xa21 and blast resistance gene, Pi54 into RPHR-1005, the restorer line of the popular rice hybrid, DRRH3

Kumar, V. Abhilash; Balachiranjeevi, C. H.; Naik, S. Bhaskar; Rambabu, R.; Rekha, G.; Madhavi, K. R.; Harika, G.; Vijay, Sonam; Pranathi, K.; Hajira, S. K.; Srivastava, Aayushi; Mahadevaswamy, H. K.; Anila, M.; Yugander, A.; Aruna, J.; Prasad, A.; Madhav, M. S.; Laha, G. S.; Viraktamath, B. C.; Balachandran, S. M.; Senguttuvel, P.; Kemparaju, B.; Babu, V. Ravindra; Sundaram, R. M.; Prasad, M. S.

doi:10.1007/s13562-016-0352-z

Cited by 12 publications

(4 citation statements)

References 28 publications

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“…As xa5 is known to exhibit partial dominance and was reported to display negative effects in gene pyramided lines of some Indian rice varieties [35], in the current study we targeted only two genes (xa13 and Xa21) out of three genes (Xa21 xa13 and xa5). ISM has also been used in the earlier molecular breeding studies towards improvement of existing rice varieties [35,38]. For the introgression of Pi54 and Pi1, in the current study used Swarnamukhi (NLR145), known to have Pi54 and Pi1 genes, as it was derived from Tetep (CICA-4 × IR-625-23-3-1 × Tetep), while the presence of Pi1 gene in NLR145 has been reported [22].…”

Section: Plos Onementioning

confidence: 99%

“…For the introgression of Pi54 and Pi1, in the current study used Swarnamukhi (NLR145), known to have Pi54 and Pi1 genes, as it was derived from Tetep (CICA-4 × IR-625-23-3-1 × Tetep), while the presence of Pi1 gene in NLR145 has been reported [22]. Tetep is already being used for introgression of Pi54 in several molecular breeding studies [39,38]. NLR145 is much better donor than Tetep, since it has less linkage drag of unwanted morphological traits.…”

Section: Plos Onementioning

confidence: 99%

“…The combinations of Pi54 and Pi1 in case of Samba Mahsuri, Swarna, Swarna sub-1 and Pusa Basmati 1 (PB 1) have showed high level of blast resistance [42][43][44][45]. Recently, many research workers have reported a highly effective blast resistance of gene pyramided lines possessing Pi54 and Pi2 in diverse locations of India [46,43,38]. Previous studies also made evident that marker assisted pyramiding of major genes have helped to increase resistance level to BB [47] and blast [25].…”

Section: Plos Onementioning

confidence: 99%

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Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

et al. 2020

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Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a welladapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F 1 to ICF 3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC 2 F 1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1-ICF 3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF 3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.

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Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

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Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

et al. 2020

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“…The xa5 + xa13 + Xa21 polyline bred by molecular marker-assisted backcross breeding strategy showed high resistance ( Pradhan et al, 2015 ). The resistance genes Xa21 and Pi54 to BB and blast were polymerized into the restorer line RPHR-1005, and the progeny materials with high resistance to BB and blast were obtained by marker-assisted backcross breeding ( Kumar et al, 2016 ). These genes showed high resistance and broad-spectrum characteristics by polymerization or combination.…”

Section: Research Prospectmentioning

confidence: 99%

Research Progress on Cloning and Function of Xa Genes Against Rice Bacterial Blight

et al. 2022

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Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases that hinder the normal growth and production of rice, which greatly reduces the quality and yield of rice. The effect of traditional methods such as chemical control is often not ideal. A series of production practices have shown that among the numerous methods for BB controlling, breeding and using resistant varieties are the most economical, effective, and environmentally friendly, and the important basis for BB resistance breeding is the exploration of resistance genes and their functional research. So far, 44 rice BB resistance genes have been identified and confirmed by international registration or reported in journals, of which 15 have been successfully cloned and characterized. In this paper, research progress in recent years is reviewed mainly on the identification, map-based cloning, molecular resistance mechanism, and application in rice breeding of these BB resistance genes, and the future influence and direction of the remained research for rice BB resistance breeding are also prospected.

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Marker‐assisted forward breeding to develop a drought‐, bacterial‐leaf‐blight‐, and blast‐resistant rice cultivar

Singh¹,

Dixit²,

Alam

et al. 2021

The Plant Genome

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Among the different challenges related to rice (Oryza sativa L.) cultivation, drought, bacterial leaf blight (BLB), and blast are the key stresses that significantly affect grain yield (GY) in rice. To ameliorate this issue, marker‐assisted forward breeding (MAFB) coupled with a simultaneous crossing approach was used to combine three drought tolerant quantitative trait loci (QTL)—qDTY1.1, qDTY3.1, and qDTY12.1—four BLB genes—Xa4, xa5, xa13, and Xa21—and one blast‐resistance gene, Pi9, in the elite rice cultivar Lalat. The introgression lines (ILs) developed in the current study were phenotypically screened for drought, BLB, and blast resistance at the F7–F8 generation. Under the reproductive stage (RS) drought stress, the yield advantage of ILs, with major‐effect QTL (qDTY) over elite parent Lalat, ranges from 9 to 124% in DS2019 and from 7 to 175% in WS2019. The selected ILs were highly resistant to BLB, with lesion lengths ranging from 1.3 to 3.0 cm and blast scores ranging from 1 to 3. ILs that were tolerant to RS drought, resistant to BLB, and blast disease and had similar or higher yields than Lalat were analyzed for grain quality. Six ILs were found to have similar grain quality characteristics to Lalat including hulling, milling, head rice recovery (HRR), chalkiness, alkali spreading value (ASV), and amylose content (AC). This study showed that MAFB, together with simultaneous crossing, would be an effective strategy to rapidly combine multiple stresses in rice. The ILs developed in this study could help to ensure yield sustainability in rainfed environments or be used as genetic material in future breeding programs.

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Marker-assisted introgression of the major bacterial blight resistance gene, Xa21 and blast resistance gene, Pi54 into RPHR-1005, the restorer line of the popular rice hybrid, DRRH3

Cited by 12 publications

References 28 publications

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

Research Progress on Cloning and Function of Xa Genes Against Rice Bacterial Blight

Marker‐assisted forward breeding to develop a drought‐, bacterial‐leaf‐blight‐, and blast‐resistant rice cultivar

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