Marker assisted introgression of blast (Pi-2 and Pi-54) genes in to the genetic background of elite, bacterial blight resistant indica rice variety, Improved Samba Mahsuri

Madhavi, K. R.; Rambabu, R.; Kumar, V. Abhilash; Kumar, Sameer; Aruna, J.; Ramesh, S.; Sundaram, R. M.; Laha, G. S.; Madhav, M. S.; Babu, V. Ravindra; Prasad, Mahendra

doi:10.1007/s10681-016-1784-1

Cited by 21 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently through application of marker-assisted backcrossing, many R genes for blast and bacterial blight have been successfully introgressed into elite inbred lines of rice to improve the disease resistance. Some new inbred varieties that combine excellent grain quality with resistance to diseases and input use efficiency have been popularized in Southeast Asia (Joseph et al 2004 ; Luo et al 2014 ; Ratna Madhavi et al 2016 ; Tanweer et al 2015 ; Xiao et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Yang

Chen

et al. 2018

Rice

View full text Add to dashboard Cite

BackgroundBreeding two-line hybrid rice with disease resistance is an effective approach to stabilize rice yield in commercial rice production of China.ResultsWe improved the blast and bacterial blight resistance of Guangzhan63-4S, an elite photoperiod- and thermo-sensitive male sterile (P/TGMS) line widely used in two-line hybrid rice, by introducing the R genes Pi2 and Xa7 conferring resistance to rice blast and bacterial blight, respectively. Through the backcrossing and gene pyramiding breeding coupled with molecular marker-assisted selection, a new P/TGMS line Hua1228S carrying Pi2, Xa7, and tms5 was developed. Based on 200,000 SNP markers by next-generation sequencing, Hua1228S covered 87.6% of the recurrent genome, as well as 4.5% of the donor genome from VE6219 and 7.9% from YR7029–39. When infected with seven tested Xanthomonas oryzae pv. oryzae strains, Hua1228S conferred high resistance (0 level) to six bacterial blight strains. Moreover, Hua1228S showed broad-spectrum resistance to rice blast isolates with a high resistance frequency of 90.91%. High levels of resistance to leaf blast and neck blast were observed under heavy disease pressure in natural field. Importantly, Hua1228S showed identical fertility-sterility alteration pattern to Guangzhan63-4S. Thus, two hybrid combinations Hua Liangyou 2821 and Hua Liangyou 284 derived from Hua1228S exhibited enhanced resistance and higher yield compared with the control variety Feng Liangyou 4.ConclusionsThese results indicate that Hua1228S has tremendous potentiality to increase and stabilize the rice yield, through the introgression of two R genes by marker-assisted selection strategy.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0203-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Yang

Chen

et al. 2018

Rice

View full text Add to dashboard Cite

show abstract

“…Eleven rice lines designated as RPNF01 through RPNF11 were used in this study. These lines were derived from a common elite variety BPT5204 (Samba Mahsuri- RPNF11- susceptible to BB, BL and GM) through systematic introgression of any of the three BB resistance genes Xa21, xa13, xa5; two of the BL resistance genes Pi2 , Pi54 and four of the GM resistance genes , Gm1, gm3, Gm4 and Gm8 in different combinations through marker-assisted backcross breeding (MABB) (Sama et al 2014 ; Madhavi et al 2016 ; Kumar et al 2017 ) as detailed in Table 1 . Foreground selection for introgression of these genes was accomplished using reported linked or gene based markers (Additional file 3 : Table S3).…”

Section: Methodsmentioning

confidence: 99%

Expression Profile of Defense Genes in Rice Lines Pyramided with Resistance Genes Against Bacterial Blight, Fungal Blast and Insect Gall Midge

Divya

Madhavi

Dass

et al. 2018

Rice

View full text Add to dashboard Cite

BackgroundRice, a major food crop of the world, endures many major biotic stresses like bacterial blight (BB), fungal blast (BL) and the insect Asian rice gall midge (GM) that cause significant yield losses. Progress in tagging, mapping and cloning of several resistance (R) genes against aforesaid stresses has led to marker assisted multigene introgression into elite cultivars for multiple and durable resistance. However, no detailed study has been made on possible interactions among these genes when expressed simultaneously under combined stresses.ResultsOur studies monitored expression profiles of 14 defense related genes in 11 rice breeding lines derived from an elite cultivar with different combination of R genes against BB, BL and GM under single and multiple challenge. Four of the genes found implicated earlier under combined GM and BB stress were confirmed to be induced (≥ 2 fold) in stem tissue following GM infestation; while one of these, cytochrome P450 family protein, was also induced in leaf in plants challenged by either BB or BL but not together. Three of the genes highlighted earlier in plants challenged by both BB and BL were also found induced in stem under GM challenge. Pi54 the target R gene against BL was also found induced when challenged by GM. Though expression of some genes was noted to be inhibited under combined pest challenge, such effects did not result in compromise in resistance against any of the target pests.ConclusionWhile R genes generally tended to respond to specific pest challenge, several of the downstream defense genes responded to multiple pest challenge either single, sequential or simultaneous, without any distinct antagonism in expression of resistance to the target pests in two of the pyramided lines RPNF05 and RPNF08.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0231-4) contains supplementary material, which is available to authorized users.

show abstract

“…oryzae) genes (Xa21, xa13, and xa5) [132]. Two major blast disease (Magnaporthe oryzae) resistance genes (Pi-2 and Pi-54) and a BB gene (Xa38) were further stacked into 'ISM' [133,134]. 'Pusa Basmati 1' pyramided with two (Pi2+Pi5) and three (Pi54+Pi1+Pita) blast genes [135] and improved version of 'Pusa Basmati 1121' and 'Pusa Basmati 6' carrying blast (Pi2 and Pi54) and BB resistance genes (xa13 and Xa21) are among others [136].…”

Section: Linking Genome Diversity and Complex Trait Phenotypesmentioning

confidence: 99%

Designing Future Crops: Genomics-Assisted Breeding Comes of Age

Varshney

Bohra

et al. 2021

Trends in Plant Science

273

197

View full text Add to dashboard Cite

Marker assisted introgression of blast (Pi-2 and Pi-54) genes in to the genetic background of elite, bacterial blight resistant indica rice variety, Improved Samba Mahsuri

Cited by 21 publications

References 27 publications

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice

Expression Profile of Defense Genes in Rice Lines Pyramided with Resistance Genes Against Bacterial Blight, Fungal Blast and Insect Gall Midge

Designing Future Crops: Genomics-Assisted Breeding Comes of Age

Contact Info

Product

Resources

About