Marker-assisted introgression of bacterial blight and blast resistance into DRR17B, an elite, fine-grain type maintainer line of rice

Balachiranjeevi, C. H.; Bhaskar, Naik S.; Abhilash, V.; Akanksha, S.; Viraktamath, B. C.; Madhav, M. S.; Hariprasad, A. S.; Laha, G. S.; Prasad, M. S.; Balachandran, S. M.; Neeraja, C. N.; Kumar, Manish; Senguttuvel, P.; Kemparaju, K. B.; Bhadana, V. P.; Ram, T.; Harika, G.; Swamy, H. K. Mahadeva; Hajira, S. K.; Yugander, A.; Pranathi, K.; Anila, M.; Rekha, G.; Kousik, M. B. V. N.; Kumar, T. Dilip; Swapnil, R. K.; Giri, Archana; Sundaram, R. M.

doi:10.1007/s11032-015-0348-8

Cited by 49 publications

(26 citation statements)

References 50 publications

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“…In this study, the recurrent parent genome recovery rates in BC 1 F 1 , BC 2 F 1 and BC 2 F 2 were 73.8%, 83% and 84.7% (Figure 2), respectively. Compared with the theoretical %RPG, a relatively low background recovery rate was obtained, however, the results were consistent with those found in previous studies [36,37]. Marker-assisted backcrossing can accelerate the breeding process and facilitate a speedy recovery for most of the recurrent genome within a few generations [38], however, the population size of each backcross generation, linkage drag, number of background markers used and genetic background between two parents are considered to be factors that reduce the efficiency of MAB and %RPG [32].…”

Section: Discussionsupporting

confidence: 91%

“…The 80% and 89% recovery rates following two and three backcrosses were obtained from three-BB-gene-pyramided BC 2 and BC 3 genotypes, via MAS [35]. Balachiranjeevi et al (2015) transferred the BB gene, Xa21 and rice blast-resistant gene, Pi54, to DRR17A and were able to recover 73.4%, 84.8% and 93.4% RPG in the BC 1 , BC 2 and BC 3 generations, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Hsu

Chiu²,

Yap

et al. 2020

IJMS

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Tainung82 (TNG82) is one of the most popular japonica varieties in Taiwan due to its relatively high yield and grain quality, however, TNG82 is susceptible to bacterial blight (BB) disease. The most economical and eco-friendly way to control BB disease in japonica is through the utilization of varieties that are resistant to the disease. In order to improve TNG82’s resistance to BB disease, five bacterial blight resistance genes (Xa4, xa5, Xa7, xa13 and Xa21) were derived from a donor parent, IRBB66 and transferred into TNG82 via marker-assisted backcrossing breeding. Five BB-resistant gene-linked markers were integrated into the backcross breeding program in order to identify individuals possessing the five identified BB-resistant genes (Xa4, xa5, Xa7, xa13 and Xa21). The polymorphic markers between the donor and recurrent parent were used for background selection. Plants having maximum contribution from the recurrent parent genome were selected in each generation and crossed with the recipient parent. Selected BC3F1 plants were selfed in order to generate homozygous BC3F2 plants. Nine pyramided plants, possessing all five BB-resistant genes, were obtained. These individuals displayed a high level of resistance against the BB strain, XF89-b. Different BB gene pyramiding lines were also inoculated against the BB pathogen, resulting in more than three gene pyramided lines that exhibited high levels of resistance. The five identified BB gene pyramided lines exhibited yield levels and other desirable agronomic traits, including grain quality and palatability, consistent with TNG82. Bacterial blight-resistant lines possessing the five identified BB genes exhibited not only higher levels of resistance to the disease, but also greater yield levels and grain quality. Pyramiding multiple genes with potential characteristics into a single genotype through marker-assisted selection can improve the efficiency of generating new crop varieties exhibiting disease resistance, as well as other desirable traits.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Hsu

Chiu²,

Yap

et al. 2020

IJMS

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“…QTLs (A,a) qSBR11-1, (B,b) In this study, no positive or negative interactions were observed between genes conferring resistance against bacterial blight and blast. Similar results were found by Sundaram et al (2008) and Balachiranjeevi et al (2015). Singh et al (2014) also reported the incorporation of multiple disease resistance including BB, rice blast and ShB in Pusa 6B and developed the new stable improved lines of the Pusa 6B.…”

Section: R R R R R Rsupporting

confidence: 80%

Marker Assisted Selection for Sheath Blight, Blast and Bacterial Blight Resistance in Two Popular Rice Varieties

Vidya

Ramalingam

2018

Madras Agricultural Journal

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Major biotic stresses that affects rice production are sheath blight (ShB) caused by Rhizoctonia solani Kuhn, blast disease caused by fungal pathogen Magnaporthe oryzae and bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae. To reduce the yield loss pyramiding of resistance (R) genes in an elite rice variety is the most effective method. Two high yielding rice varieties namely, ASD16 and ADT43 are extensively grown in South India. These varieties are susceptible to blast, bacterial blight and sheath blight diseases. Hence this study aims to improve these disease susceptible varieties to achieve higher yield in disease prone areas. Improved ASD16 (CB14004) harbouring BB and blast and improved ADT43 (CB14002) harbouring BB resistance genes were used in the present marker assisted backcross breeding program to introgress three sheath blight resistant QTLs (qSBR 11-1, qSBR 11-2 and qSBR 7-1). In every backcross generation, PCR-based markers (RM224, RM209 and RM336), linked with sheath blight resistance QTLs, bacterial blight resistance genes (xa5, xa13 and Xa21) and blast resistance genes (Pi54) were utilized for foreground selection. In addition, a set of forty eight (CB14002 x tetep) and forty one (CB14002) parental polymorphic SSR markers were used for background selection and backcrossing was carried out until BC 3 generation. Background selection studies using polymorphic markers revealed that, an average recovery of 91.15% and the maximum of 92.68% recurrent parent genome in CB14004 x Tetep cross population. In CB14002 x Tetep an average of 90.20% and maximum of 92.70% has been recovered. These plants will be selfed to generate large number of segregating progenies to identify agronomically superior with good grain quality traits and multiple disease resistance lines in rice.

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“…Therefore, rice blast resistance has been considered as one of the priorities in the rice breeding program. R-gene linked or specific molecular markers were developed and widely used in the mainstream breeding programs via marker aided selection (MAS) (Jiang et al 2012;Balachiranjeevi et al 2015;Ni et al 2015;Shalini et al 2016;Wu et al 2016;Mi et al 2018). However, many rice blast R-gene haplotypes from different rice lines are almost identical except for a few nucleotide differences, leading to different specificities or even loss of function, e.g, different Pik and Pi2/9 alleles (Zhou et al 2007;Constanzo and Jia 2010).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

Xiao

Jiao

Zhu

et al. 2020

Rice

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Multiple haplotypes at the same rice blast R-gene locus share extremely high sequence similarity, which makes the gene diagnostic method using molecular markers less effective in differentiation from one another. The composition and distribution pattern of deployed R genes/haplotypes in elite rice varieties has not been extensively analyzed. In this study, we employed PCR amplification and sequencing approach for the diagnosis of R-gene haplotypes in 54 Chinese elite rice varieties. A varied number of functional and nonfunctional haplotypes of 4 target major R-gene loci, i.e., Pi2/9, Pi5, Pik, and Pib, were deduced by referring to the reference sequences of known R genes. Functional haplotypes accounted for relatively low frequencies for the Pi2/9 (15%) and Pik (9%) loci but for relatively high frequencies for the Pi5 (50%) and Pib (54%) loci. Intriguingly, significant frequencies of 33%, 39%, 46% of non-functional haplotypes at the Pi2/9, Pik, and Pib loci, respectively, with traceable original donors were identified, suggesting that they were most likely unintentionally spread by using undesirable donors in various breeding programs. In the case of Pi5 locus, only a single haplotype, i.e., Pi5 was identified. The reactions of 54 rice varieties to the differential isolates were evaluated, which showed a good correlation to the frequency of cognate avirulence (Avr) genes or haplotypes in the differential isolates. Four R genes, i.e., Pi2, Piz-t, Pi50, and Pikm were found to contribute significantly to the resistance of the elite rice varieties. Other two genes, Pi9 and Pikh, which were not utilized in rice varieties, showed promising values in breeding durable resistance due to their high resistance frequencies to the contemporary rice blast population. The sequencebased molecular diagnosis provided a promising approach for the identification and verification of haplotypes in different R-gene loci and effective R genes valuable for breeding durable rice resistance to rice blast.

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Marker-assisted introgression of bacterial blight and blast resistance into DRR17B, an elite, fine-grain type maintainer line of rice

Cited by 49 publications

References 50 publications

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Marker Assisted Selection for Sheath Blight, Blast and Bacterial Blight Resistance in Two Popular Rice Varieties

Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

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