Marker assisted gene pyramiding for enhanced Tomato leaf curl virus disease resistance in tomato cultivars

Abstract

The present research is aimed towards molecular marker assisted pyramiding Tomato leaf curl virus (ToLCV) disease resistance genes into two ToLCV susceptible tomato (Solanum lycopersicum L.) cvs. Pbc and H-86 (resistance genes recipient parents). Resistance gene donors were EC-538408 (Solanum chilense) and EC-520061 (S. peruvianum) in the case of cv. Pbc, and EC-520061 (S. peruvianum) and H-24 (S. lycopersicum) in the case of cv. H-86. A ToLCV resistance gene associated co-dominant simple sequence repeat (SSR) marker SSR-218 was used to discriminate between homozygotes and heterozygotes at the seedling stage prior to pollination, which enabled the rejection of nontarget back crosses and pyramiding progenies of the crosses PbcxEC-520061 and H-86xEC-520061, whereas SSR-306 was used for the cross PbcxEC-538408. Ty-2 gene cleaved amplified polymorphic sequences (CAPS) marker was used for the cross H-86xH-24. Out of 279 pyramiding progenies of the cross PbcxEC-538408/PbcxEC-520061, total 91 plants showed the presence of both resistance allele 1 and 2 along with both susceptibility alleles, and in 243 pyramiding progenies of the cross H-86xEC-520061/H-86xH-24, total 82 plants showed the presence of both resistance allele 1 and Ty-2 along with both susceptible alleles. The pyramiding lines that carried both pyramided resistance genes were resistant to tomato leaf curl disease throughout its life cycle.

A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

“…Nevertheless, if efficient spread to tomato occurs, here we showed that the Ty-1 gene already deployed for the control TYLCD-associated viruses [49] seems to provide resistance to ToLCNDV, even for the isolate from India that causes severe infections in susceptible tomatoes. Moreover, other gene combinations have been reported to provide effective resistance to ToLCNDV infections in tomato [50,51]. …”

Genome-Wide Association Study of Resistance to Soybean Cyst Nematode (Heterodera glycines) HG Type 2.5.7 in Wild Soybean (Glycine soja)

“…Wild crop relatives harbor novel and exotic genetic resources that are crucial for the development of insect pest management (Hajjar and Hodgkin, 2007 ). Some wild species have been applied in breeding practices, such as for tomato (Vidavski et al, 2008 ; Kumar et al, 2014 ). G. soja is widely distributed in China, Korea, Japan, and Northeast Russia, and long-term exposure to various environmental stresses in the wild has rendered G. soja able to adapt to diverse environments with severe abiotic and biotic stresses.…”

Back into the wild—Apply untapped genetic diversity of wild relatives for crop improvement

“…As one example, five Ty genes exhibiting varying degrees of resistance to tomato yellow leaf curl virus (TYLCY) were successfully introgressed into cultivated varieties (Ji, Scott, Hanson, Graham, & Maxwell, 2007;Menda et al, 2014). Pyramiding of these Ty genes from different wild tomatoes has contributed to durable and broad resistance to TYLCY (Kumar, Tiwari, Datta, & Singh, 2014;Vidavski, Czosnek, Gazit, Levy, & Lapidot, 2008).…”