Marker assisted detection and LC-MS analysis of antimicrobial compounds in different Bacillus strains and their antifungal effect on Sclerotinia sclerotiorum

Farzand, Ayaz; Moosa, Anam; Zubair, Muhammad; Khan, Abdur Rashid; Hanif, Alvina; Tahir, Hafiz Abdul Samad; Gao, Xuewen

doi:10.1016/j.biocontrol.2019.03.014

Cited by 46 publications

(46 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibiosis is the main mechanism of biological control that has been well-studied genetically and biochemically [35]. In recent years, several studies have reported the inhibition of fungal pathogens by cyclic lipopeptides [6,37,38]. The excretion of antimicrobial compounds by Bacillus species is the primary cause of the formation of fungal growth inhibition zones in dual culture [39].…”

Section: Discussionmentioning

confidence: 99%

Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42

et al. 2019

Self Cite

View full text Add to dashboard Cite

Lipopeptides from Bacillus species exhibit promising biological control activity against plant pathogens. This study aimed to explore the potential of purified fengycin to induce systemic resistance in tomato against Sclerotinia sclerotiorum. Bacillus amyloliquefaciens FZB42, its mutant AK1S, and their corresponding metabolites showed in vitro inhibition of S. sclerotiorum mycelium. Fengycin derived from an AK1S mutant was purified and identified through HPLC and MALDI-TOF-MS, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed structural deformities in the fungal mycelium. Moreover, fengycin induced the accumulation of reactive oxygen species (ROS) in S. sclerotiorum mycelium and downregulated the expression of ROS-scavenging genes viz., superoxide dismutase (SsSOD1), peroxidase (SsPO), and catalase (SsCAT1) compared to the untreated control. Furthermore, the lesion size was dramatically reduced in fengycin-treated tomato plants compared to plants infected with S. sclerotiorum only in a greenhouse experiment. Additionally, the transcriptional regulation of defense-related genes GST, SOD, PAL, HMGR, and MPK3 showed the highest upsurge in expression at 48 h post-inoculation (hpi). However, their expression was subsequently decreased at 96 hpi in fengycin + S. sclerotiorum treatment compared to the plants treated with fengycin only. Conversely, the expression of PPO increased in a linear manner up to 96 hpi.

show abstract

Section: Discussionmentioning

confidence: 99%

Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…An amount of 2 µL of sample was injected into the machine having with electrospray ionization (ESI) in positive ion mode [M+H] + with MSE acquisition in the range of 50-1200 m/z. A similar run phase and protocol were used as already reported by our lab [78]. The data collection and analysis were done using Mass Lynx software (Version 4.1) (https://www.waters.com/waters/en_US/MassLynx-Mass-Spectrometry-Software-/nav.htm?cid=513164&locale=en_US accessed on 2 November 2019).…”

Section: Liquid Chromatography-mass Spectrometry (Lc-ms) Analysis Under Low Temperaturementioning

confidence: 99%

Novel Genetic Dysregulations and Oxidative Damage in Fusarium graminearum Induced by Plant Defense Eliciting Psychrophilic Bacillus atrophaeus TS1

Zubair

Farzand

Mumtaz

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

This study elaborates inter-kingdom signaling mechanisms, presenting a sustainable and eco-friendly approach to combat biotic as well as abiotic stress in wheat. Fusarium graminearum is a devastating pathogen causing head and seedling blight in wheat, leading to huge yield and economic losses. Psychrophilic Bacillus atrophaeus strain TS1 was found as a potential biocontrol agent for suppression of F. graminearum under low temperature by carrying out extensive biochemical and molecular studies in comparison with a temperate biocontrol model strain Bacillus amyloliquefaciens FZB42 at 15 and 25 °C. TS1 was able to produce hydrolytic extracellular enzymes as well as antimicrobial lipopeptides, i.e., surfactin, bacillomycin, and fengycin, efficiently at low temperatures. The Bacillus strain-induced oxidative cellular damage, ultrastructural deformities, and novel genetic dysregulations in the fungal pathogen as the bacterial treatment at low temperature were able to downregulate the expression of newly predicted novel fungal genes potentially belonging to necrosis inducing protein families (fgHCE and fgNPP1). The wheat pot experiments conducted at 15 and 25 °C revealed the potential of TS1 to elicit sudden induction of plant defense, namely, H2O2 and callose enhanced activity of plant defense-related enzymes and induced over-expression of defense-related genes which accumulatively lead to the suppression of F. graminearum and decreased diseased leaf area.

show abstract

“…Based on a nontarget LC‐HRMS study, we identified 11 different bioactive compounds between the two consortia based on the accurate monoisotopic molecular weight (Table 1 and Figure A3). To do this, we used an in‐house database with biomarkers from different Bacillus strains retrieved from the literature (Farzand et al, 2019), because the main group of bacteria in kefir is Lactobacillus spp. (Dobson et al, 2011; Gao & Zhang, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…MetLin comprises 29,000 exogenous and endogenous natural products from diverse sources, including actinomycetes. Alternatively, an in‐house Bacillus and Gordonia bioactive metabolite database was built after retrieving data reported in the literature (Farzand et al, 2019; Nagao et al, 2001; Pan et al, 2019; Phister et al, 2004; Xu et al, 2018), using the PCLD program in the Masshunter program (Agilent Technologies). Special care was taken with the sodium and potassium adducts that sometimes form in an MS analysis.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Metagenomic analysis and antimicrobial activity of two fermented milk kefir samples

et al. 2021

View full text Add to dashboard Cite

In recent years, the fermented milk product kefir has been intensively studied because of its health benefits. Here, we evaluated the microbial consortia of two kefir samples, from Escarcega, Campeche, and Campeche (México). We considered a functional comparison between both samples, including fungal and bacterial inhibition; second, we applied shotgun metagenomics to assess the structure and functional diversity of the communities of microorganisms. These two samples exhibited antagonisms against bacterial and fungal pathogens. Bioactive polyketides and nonribosomal peptides were identified by LC‐HRMS analysis. We also observed a high bacterial diversity and an abundance of Actinobacteria in both kefir samples, and a greater abundance of Saccharomyces species in kefir of Escarcega than in the Campeche kefir. When the prophage compositions were evaluated, the Campeche sample showed a higher diversity of prophage sequences. In Escarcega, we observed a prevalence of prophage families that infect Enterobacteria and Lactobacillus. The sequences associated with secondary metabolites, such as plipastatin, fengycin, and bacillaene, and also bacteriocins like helveticin and zoocin, were also found in different proportions, with greater diversity in the Escarcega sample. The analyses described in this work open the opportunity to understand the microbial diversity in kefir samples from two distant localities.

show abstract

Marker assisted detection and LC-MS analysis of antimicrobial compounds in different Bacillus strains and their antifungal effect on Sclerotinia sclerotiorum

Cited by 46 publications

References 60 publications

Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42

Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42

Novel Genetic Dysregulations and Oxidative Damage in Fusarium graminearum Induced by Plant Defense Eliciting Psychrophilic Bacillus atrophaeus TS1

Metagenomic analysis and antimicrobial activity of two fermented milk kefir samples

Contact Info

Product

Resources

About