Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus)

Fuji, Kanako; Hasegawa, Osamu; Honda, Kazumitsu; Kumasaka, Kiyohiro; Sakamoto, Takashi; Okamoto, Nobuaki

doi:10.1016/j.aquaculture.2007.07.210

Cited by 127 publications

(73 citation statements)

References 19 publications

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“…Fuji et al [42] used a segregated population and located a single major QTL controlling resistance to lymphocystis disease on LG15 in Japanese flounder with a phenotypic variation value of 50%. Further study confirmed that an SSR locus within that QTL was closely related with a phenotype for lymphocystis disease resistance [43].…”

Section: Qtl For Disease Resistancesupporting

confidence: 59%

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Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish

Tong

Sun

2015

Sci. China Life Sci.

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The traits of cultured fish must continually be genetically improved to supply high-quality animal protein for human consumption. Economically important fish traits are controlled by multiple gene quantitative trait loci (QTL), most of which have minor effects, but a few genes may have major effects useful for molecular breeding. In this review, we chose relevant studies on some of the most intensively cultured fish and concisely summarize progress on identifying and verifying QTLs for such traits as growth, disease and stress resistance and sex in recent decades. The potential applications of these major-effect genes and their associated markers in marker-assisted selection and molecular breeding, as well as future research directions are also discussed. These genetic and genomic analyses will be valuable for elucidating the mechanisms modulating economically important traits and to establish more effective molecular breeding techniques in fish.aquaculture fish, economic traits, QTL, major genes, molecular markers, molecular breeding Citation:Tong JG, Sun XW. Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish. Sci

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Section: Qtl For Disease Resistancesupporting

confidence: 59%

“…Fuji et al [42] found an SSR allele associated with IPN, and used it during large-scale anti-IPN selective breeding and fingerling production [43]. Selective breeding of gibeio carp has made good progress using anti-myxosporean-related MHC alleles as molecular markers [79].…”

Section: Progress Of Applied Molecular Breeding Studiesmentioning

confidence: 99%

Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish

Tong

Sun

2015

Sci. China Life Sci.

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“…This species has been cultured since the 1990s and is an important marine fish in East Asia. Multiple breeding methods are currently in use to prevent genetic degradation in cultured olive flounder stocks, including artificial sex control, polyploidy breeding, and marker-assisted breeding (Fuji et al 2007;Yamamoto 1999). However, these breeding methods are time-consuming, require large amounts of space, and cannot be used to introduce genetic modifications.…”

Section: Introductionmentioning

confidence: 99%

A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3

Tan

Jiao

et al. 2015

Dev Genes Evol

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The olive flounder (Paralichthys olivaceus) is an important cultured marine fish. However, little information is available on primordial germ cell (PGC) development and migration in this species; such information is vital for applications in artificial reproduction and for the preservation of genetic resources. Here, we sought to remedy this information deficit by isolating the germline-specific gene nanos3 and analyzing its expression in olive flounder. Sequencing analysis showed that olive flounder nanos3 contained a typical RNA-binding zinc finger domain. A phylogenetic analysis demonstrated that nanos3 of the olive flounder grouped with that of the barfin flounder (Verasper moseri). In the olive flounder, nanos3 was consistently expressed during embryogenesis. Whole-mount in situ hybridization showed that a new pattern of PGC migration was present in olive flounder, which combined elements of the PGC migration patterns of medaka, herring, and goby. In olive flounder, PGCs aligned along the lateral plate mesoderm in two loose, elongated lines at early embryogenesis, aggregated into a single loose cluster at mid-embryogenesis, then re-aligned into two tight clusters at late somitogenesis, and finally migrated to the genital ridge as two clusters. Furthermore, whole-mount in situ hybridization revealed that expression of stromal derived factor 1 (Sdf1) was important for guiding of PGC migration during somitogenesis. In particular, Sdf1 directed aggregation of PGCs into a single loose cluster from the two elongated lines during mid-embryogenesis. Additionally, PGCs in zebrafish were successfully visualized by injection of chimeric RNA containing the green fluorescent protein (GFP) and 3' untranslated region of olive flounder nanos3. These findings provide new insights into PGC migration and development in olive flounder and will also facilitate germ cell manipulation in this species.

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“…In the tongue sole, a female-specific DNA marker, located in the sex-linked LG5 (Liao et al 2009), has also been identified (Chen et al 2007). Regarding disease resistance, Aeromonas salmonicida resistance-related traits and a major locus on LG15 (marker Poli.9-8TUF) for resistance to lymphocystis disease have been reported in turbot (Rodríguez-Ramilo et al 2011) and Japanese flounder (Fuji et al 2006(Fuji et al , 2007, respectively. The latter trait in Japanese flounder has been successfully applied as a selection breeding marker.…”

Section: Genetic Maps and Detection Of Quantitative Trait Loci (Qtls)mentioning

confidence: 99%

Advances in genomics for flatfish aquaculture

Cerdà

Manchado

2012

Genes Nutr

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Fish aquaculture is considered to be one of the most sustainable sources of protein for humans. Many different species are cultured worldwide, but among them, marine flatfishes comprise a group of teleosts of high commercial interest because of their highly prized white flesh. However, the aquaculture of these fishes is seriously hampered by the scarce knowledge on their biology. In recent years, various experimental 'omics' approaches have been applied to farmed flatfishes to increment the genomic resources available. These tools are beginning to identify genetic markers associated with traits of commercial interest, and to unravel the molecular basis of different physiological processes. This article summarizes recent advances in flatfish genomics research in Europe. We focus on the new generation sequencing technologies, which can produce a massive amount of DNA sequencing data, and discuss their potentials and applications for de novo genome sequencing and transcriptome analysis. The relevance of these methods in nutrigenomics and foodomics approaches for the production of healthy animals, as well as high quality and safety products for the consumer, is also briefly discussed.

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Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus)

Cited by 127 publications

References 19 publications

Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish

Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish

A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3

Advances in genomics for flatfish aquaculture

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