Marker assisted breeding for transformability in maize

Lowe, Brenda; Way, Melissa M.; Kumpf, Jennifer M.; Rout, Jyoti; Warner, Dave; Johnson, R. C.; Armstrong, Charles; Spencer, Michael; Chomet, Paul

doi:10.1007/s11032-006-9031-4

Cited by 23 publications

(15 citation statements)

References 14 publications

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“…One marker, bnlg1160, was estimated between 187,043,888 and 188,417,593 (Andorf et al, 2010). The QTL reported for tissue culture traits involving other genotypes including H99 and two Chinese maize genotypes, 18 through 599 and Huangzao4, also include loci on chromosome 3 attributing to genetic contributions to culture response (Armstrong et al, 1992; Guangtang et al, 2006; Krakowsky et al, 2006; Lowe et al, 2006; Pan et al, 2006; Zhang et al, 2006).…”

Section: A Summary Of Quantitative Trait Loci (Qtl) Mapping Studies Omentioning

confidence: 99%

Genetic Fine‐Mapping of a Quantitative Trait Locus (QTL) Associated with Embryogenic Tissue Culture Response and Plant Regeneration Ability in Maize (Zea mays L.)

et al. 2018

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Embryogenic and regenerable tissue cultures are widely utilized in plant transformation, clonal propagation, and biological research applications. Germplasm utilized in those applications are limited, however, due to genotype-dependent culture response. The goal of this study was to identify genomic regions controlling embryogenic and regenerable tissue culture response in the globally important crop, maize ( L.), toward the long-term objective of developing approaches for genotype-independent plant genetic engineering and clonal propagation systems. An inbred maize line, WCIC2, nearly-isogenic to reference inbred B73, was developed by phenotypic selection and molecular marker analysis. WCIC2 has over 50x increase in tissue culture response relative to the recurrent parent, B73. This line was used to genetically fine-map a region on chromosome 3 controlling embryogenic and regenerable tissue culture response to a 23.9 Mb region. WCIC2 and derivatives will be useful materials to enable maize research in a genetic background similar to B73, and our genetic mapping results will advance research to identify causal genes controlling somatic embryo formation and plant regeneration in maize.

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Section: A Summary Of Quantitative Trait Loci (Qtl) Mapping Studies Omentioning

confidence: 99%

Genetic Fine‐Mapping of a Quantitative Trait Locus (QTL) Associated with Embryogenic Tissue Culture Response and Plant Regeneration Ability in Maize (Zea mays L.)

et al. 2018

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“…Comparative mapping of SDRs and haplotype examination underlying these SDRs will also lead to the detection of those SDRs that most commonly affect segregation distortion in barley and indicate which may be most rewarding to target in projects aiming to clone the gene causing distortion (Fu et al 2006;Han et al 2006) or those aiming at improving the success of DH line production efficiency (Lowe et al 2006).…”

Section: Segregation Distortion and Haplotype Analysismentioning

confidence: 99%

Construction of a high-density composite map and comparative mapping of segregation distortion regions in barley

Kilian²,

Zhou

et al. 2010

Mol Genet Genomics

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Segregation distortion can negatively impact on gains expected using selection. In order to increase our understanding of genetic factors that may influence the extent and direction of segregation distortion, segregation distortion analyses were conducted in four different doubled haploid (DH) populations. A high-density composite map of barley was then constructed by integrating information from the four populations. The composite map contained 2,111 unique loci, comprising RFLP, SSR and DArT markers and spanned 1,136 cM. In the four populations investigated, the proportion of markers with segregation distortion ranged from 15 to 38%, depending on the population. The highest distortion was observed in populations derived by the microspore culture technique. Distorted loci tended to be clustered, which allowed definition of segregation distortion regions (SDRs). A total of 14 SDRs were identified in the 4 populations. Using the high-density composite map, several SDRs were shown to have consistent map locations in two or more populations; one SDR on chromosome 1H was present in all four populations. The analysis of haplotypes underlying seven SDRs indicated that in three cases the under-represented haplotypes were common across populations, but for four SDRs the under-represented haplotypes varied across populations. Six of the seven centromeric regions harboured SDRs suggesting that genetic processes related to position near a centromere caused the segregation distortion in these SDRs. Other SDRs were most likely due to the methods used to produce the DH populations. The association of the SDRs identified in this study and some of the genes involved in the process of haploid production described in other studies were compared. The composite map constructed in this study provides an additional resource for the barley community via increased genome coverage and the provision of additional marker options. It has also enabled further insights into mechanisms that underpin segregation distortion.

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“…At least one new locus was found that controls the production of somatic embryogenesis in A188. Lowe et al [ 176 ] reported marker-assisted breeding (MAB) for transformability in maize. Maize lines with improved culturability and transformability were produced using MAB to introgress specific regions from the highly transformable hybrid, Hi-II, into the elite line, FBLL that responds very poorly in culture.…”

Section: Transgenicsmentioning

confidence: 99%

Advances in Maize Genomics and Their Value for Enhancing Genetic Gains from Breeding

Skinner

et al. 2009

International Journal of Plant Genomics

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Maize is an important crop for food, feed, forage, and fuel across tropical and temperate areas of the world. Diversity studies at genetic, molecular, and functional levels have revealed that, tropical maize germplasm, landraces, and wild relatives harbor a significantly wider range of genetic variation. Among all types of markers, SNP markers are increasingly the marker-of-choice for all genomics applications in maize breeding. Genetic mapping has been developed through conventional linkage mapping and more recently through linkage disequilibrium-based association analyses. Maize genome sequencing, initially focused on gene-rich regions, now aims for the availability of complete genome sequence. Conventional insertion mutation-based cloning has been complemented recently by EST- and map-based cloning. Transgenics and nutritional genomics are rapidly advancing fields targeting important agronomic traits including pest resistance and grain quality. Substantial advances have been made in methodologies for genomics-assisted breeding, enhancing progress in yield as well as abiotic and biotic stress resistances. Various genomic databases and informatics tools have been developed, among which MaizeGDB is the most developed and widely used by the maize research community. In the future, more emphasis should be given to the development of tools and strategic germplasm resources for more effective molecular breeding of tropical maize products.

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Marker assisted breeding for transformability in maize

Cited by 23 publications

References 14 publications

Genetic Fine‐Mapping of a Quantitative Trait Locus (QTL) Associated with Embryogenic Tissue Culture Response and Plant Regeneration Ability in Maize (Zea mays L.)

Genetic Fine‐Mapping of a Quantitative Trait Locus (QTL) Associated with Embryogenic Tissue Culture Response and Plant Regeneration Ability in Maize (Zea mays L.)

Construction of a high-density composite map and comparative mapping of segregation distortion regions in barley

Advances in Maize Genomics and Their Value for Enhancing Genetic Gains from Breeding

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