Marker-Assisted Breeding for Economic Traits in Common Bean

Kelly, James D.; Bornowski, Nolan

doi:10.1007/978-3-319-94746-4_10

Cited by 21 publications

(14 citation statements)

References 122 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, in recent years, use of GWAS for mapping economically important QTLs in agricultural crops has increased and P. vulgaris is not behind. Many QTLs having economic importance in common bean have been mapped using a GWAS approach ( Moghaddam et al, 2016 ; Wu et al, 2017 ; Kelly and Bornowski, 2018 ; Kamfwa et al, 2019 ; Bisneta and Gonçalves-Vidigal, 2020 ; Bornowski et al, 2020 ; Gonçalves-Vidigal et al, 2020 ; Mungalu et al, 2020 ). In the present study, significant differences observed in NW Indian bean genotypes led to the identification of SNPs associated with the anthracnose resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Anthracnose resistance genes Co-3 and its alleles, Co-16 are mapped to common bean Pv04 and this study also found association of SNPs on Pv04 with anthracnose resistance to races 3, 87, and 503 ( Geffroy et al, 1999 ; David et al, 2008 ; Coimbra-Gonçalves et al, 2016 ; Murube et al, 2019 ). The Co-3 4 locus was previously mapped on the 3.36-Mb genomic position but it was recently mapped to the region between 0.49 to 0.58 Mb on Pv04 ( Valentini et al, 2017 ) which indicates shift in the physical positions with better mapping tools ( Kelly and Bornowski, 2018 ). A previous GWAS study with an Andean diversity panel by Zuiderveen et al (2016) identified two SNPs located at 0.45 and 0.53 Mb on Pv04 against race 7 and 109, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…This region contains many NBS-LRR genes ( Richard et al, 2017 ). The Co-2 gene was previously mapped at 39.73 Mb ( Creusot et al, 1999 ; Zuiderveen et al, 2016 ), but a recent study fine mapped it to 51.52 Mb ( Kelly and Bornowski, 2018 ; Miklas et al, 2020 ). There is a tight association between Co-2 and Ur-11 , a bean rust resistance gene ( Awale et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

et al. 2020

View full text Add to dashboard Cite

Common bean ( Phaseolus vulgaris L.) is an important legume crop of north-western (NW) Himalayan region and the major disease that causes catastrophic loss to the crop is anthracnose, which is caused by Colletotrichum lindemuthianum . The pathogen is highly diverse and most of the commercial cultivars are susceptible to different races prevalent in the region. The lack of information on the genomic regions associated with anthracnose resistance in NW Himalayan common bean population prompted us to dissect Quantitative Resistance Loci (QRLs) against major anthracnose races. In this study, 188 common bean landraces collected from NW region were screened against five important anthracnose races and 113 bean genotypes showed resistance to one or multiple races. Genotyping by sequencing (GBS) was performed on a panel of 192 bean lines (4 controls plus 188 Indian beans) and 22,589 SNPs were obtained that are evenly distributed. Population structure analysis of 192 bean genotypes categorized 188 Indian beans into two major clusters representing Andean and Mesoamerican gene pools with obvious admixtures. Many QRLs associated with anthracnose resistance to Indian C. lindemuthianum virulences (race 3, 87, and 503) are located at Pv04 within the gene models that encode typical resistance gene signatures. The QRLs associated with race 73 are located on Pv08 and overlaps with Co-4 anthracnose resistance gene. A SNP located at distal end of Pv11 in a gene model Phvul.011G202300 which encodes a LRR with a typical NB-ARC domain showed association with race 73 resistance. Common bean genomic regions located at Pv03, Pv09, and Pv11 showed association with resistance to anthracnose race 2047. The present study showed presence of many novel bean genomic regions associated with anthracnose resistance. The presence of Co-4 and Co-2 genes in our material is encouraging for breeding durable anthracnose resistant cultivars for the region.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The African Orphan Crop Consortium (AOCC) has included bambara groundnut in their 101 traditional African food crops with nutritional potential in which genomic resources would help to improve their nutrient content. In contrast, common bean which is the most consumed legume (Schmutz et al 2014) has had molecular-assisted breeding approaches applied for more than two decades; from as early as the 1990s, utilising random amplified polymorphic DNA (RAPD) markers linked to disease-resistance traits (summarised by Kelly and Bornowski 2018). The genetic diversity of a number of bambara groundnut landraces was initially evaluated through RAPD and amplified fragment length polymorphism (AFLP) markers (Amadou et al 2001;Massawe et al 2003a, b;Rungnoi et al 2012;Fatimah et al 2018).…”

Section: Molecular Tools and Resourcesmentioning

confidence: 99%

Bambara groundnut: an exemplar underutilised legume for resilience under climate change

Mayes

Chai

et al. 2019

Planta

122

118

View full text Add to dashboard Cite

Main conclusion Bambara groundnut has the potential to be used to contribute more the climate change ready agriculture. The requirement for nitrogen fixing, stress tolerant legumes is clear, particularly in low input agriculture. However, ensuring that existing negative traits are tackled and demand is stimulated through the development of markets and products still represents a challenge to making greater use of this legume.

show abstract

“…A summary of the broad breeding objectives in common bean improvement was recently reviewed by Assefa [6]. Abundant genetic diversity, easy germplasm accessibility, and application of marker assisted breeding technologies have supported conventional bean breeding efforts [3,7,8]. In addition, advances in whole genome sequencing and transcriptome analysis have paved a way for genomics-enabled bean breeding through either conventional approaches or genetic engineering [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.)

et al. 2020

Self Cite

View full text Add to dashboard Cite

Stable transformation of common bean (Phaseolus vulgaris L.) has been successful, to date, only using biolistic-mediated transformation and shoot regeneration from meristemcontaining embryo axes. In this study, using precultured embryo axes, and optimal co-cultivation conditions resulted in a successful transformation of the common bean cultivar Olathe using Agrobacterium tumefaciens strain EHA105. Plant regeneration through somatic embryogenesis was attained through the preculture of embryo axes for 12 weeks using induced competent cells for A. tumefaciens-mediated gene delivery. Using A. tumefaciens at a low optical density (OD) of 0.1 at a wavelength of 600 nm for infection and 4-day co-cultivation, compared to OD 600 of 0.5, increased the survival rate of the inoculated explants from 23% to 45%. Selection using 0.5 mg L-1 glufosinate (GS) was effective to identify transformed cells when the bialaphos resistance (bar) gene under the constitutive 35S promoter was used as a selectable marker. After an 18-week selection period, 1.5%-2.5% inoculated explants, in three experiments with a total of 600 explants, produced GS-resistant plants through somatic embryogenesis. The expression of bar was confirmed in first-and secondgeneration seedlings of the two lines through reverse polymerase chain reaction. Presence of the bar gene was verified through genome sequencing of two selected transgenic lines. The induction of regenerable, competent cells is key for the successful transformation, and the protocols described may be useful for future transformation of additional Phaseolus germplasm.

show abstract

Marker-Assisted Breeding for Economic Traits in Common Bean

Cited by 21 publications

References 122 publications

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

Bambara groundnut: an exemplar underutilised legume for resilience under climate change

Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.)

Contact Info

Product

Resources

About