Marked gut microbiota dysbiosis and increased imidazole propionate are associated with a NASH Göttingen Minipig model

Lützhøft, Ditte Olsen; Sinioja, Tim; Christoffersen, Berit Østergaard; Jakobsen, Rasmus Riemer; Geng, D. Y.; Ahmad, Hajar Fauzan; Straarup, Ellen Marie; Pedersen, Karen-Margrethe; Kot, Witold; Pedersen, Henrik D.; Cirera, Susanna; Hyötyläinen, Tuulia; Nielsen, Dennis Sandris; Hansen, Axel Kornerup

doi:10.1186/s12866-022-02704-w

Cited by 7 publications

(1 citation statement)

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“…A few studies have focused on gut microbiota in various minipig models. These studies support the minipig as a relevant model to study hostmicrobiota interaction [18][19][20].…”

Section: Introductionsupporting

confidence: 72%

High fat diet is associated with gut microbiota dysbiosis and decreased gut microbial derived metabolites related to metabolic health in young Göttingen Minipigs

Lützhøft,

Bækgård,

Wimborne

et al. 2024

PLoS ONE

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The objectives were 1) to characterize a Göttingen Minipig model of metabolic syndrome regarding its colon microbiota and circulating microbial products, and 2) to assess whether ovariectomized female and castrated male minipigs show similar phenotypes. Twenty-four nine-week-old Göttingen Minipigs were allocated to four groups based on sex and diet: ovariectomized females and castrated males fed either chow or high-fat diet (HFD) for 12 weeks. At study end, body composition and plasma biomarkers were measured, and a mixed meal tolerance test (MMT) and an intravenous glucose tolerance test (IVGTT) were performed. The HFD groups had significantly higher weight gain, fat percentage, fasting plasma insulin and glucagon compared to the chow groups. Homeostatic model assessment of insulin resistance index (HOMA-IR) was increased and glucose effectiveness derived from the IVGTT and Matsuda´s insulin sensitivity index from the MMT were decreased in the HFD groups. The HFD groups displayed dyslipidemia, with significantly increased total-, LDL- and HDL-cholesterol, and decreased HDL/non-HDL cholesterol ratio. The colon microbiota of HFD minipigs clearly differed from the lean controls (GuniFrac distance matrix). The main bacteria families driving this separation were Clostridiaceae, Fibrobacteraceae, Flavobacteriaceae and Porphyromonadaceae. Moreover, the species richness was significantly decreased by HFD. In addition, HFD decreased the circulating level of short chain fatty acids and beneficial microbial metabolites hippuric acid, xanthine and trigonelline, while increasing the level of branched chain amino acids. Six and nine metabolically relevant genes were differentially expressed between chow-fed and HFD-fed animals in liver and omental adipose tissue, respectively. The HFD-fed pigs presented with metabolic syndrome, gut microbial dysbiosis and a marked decrease in healthy gut microbial products and thus displayed marked parallels to human obesity and insulin resistance. HFD-fed Göttingen Minipig therefore represents a relevant animal model for studying host-microbiota interactions. No significant differences between the castrated and ovariectomized minipigs were observed.

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“…A few studies have focused on gut microbiota in various minipig models. These studies support the minipig as a relevant model to study hostmicrobiota interaction [18][19][20].…”

Section: Introductionsupporting

confidence: 72%