Marinomonas basaltis sp. nov., a marine bacterium isolated from black sand

Abstract

A Gram-negative, aerobic, slightly halophilic, rod-shaped bacterium was isolated from black sand in Soesoggak, Jeju island, Korea. The strain, designated J63 T , was oxidase-and catalase-positive and arginine dihydrolase-negative. The isolate required Na + for growth and differed from phenotypically related species by being able to utilize sucrose and D-galactose as a carbon source. Phylogenetic analysis based on the sequence of the 16S rRNA gene revealed that strain J63 T belongs to the genus Marinomonas. It exhibited 16S rRNA gene sequence similarities of 97.6-98.7 % to the closely related species Marinomonas communis, Marinomonas ostreistagni, Marinomonas aquimarina and Marinomonas vaga. The phylogenetic analysis revealed that strain J63 T comprised a relatively long subline of descent, shared a branch point with the outlying species Marinomonas communis and occupied a phylogenetically distant position on the main Marinomonas branch. Based on DNA-DNA hybridization, the levels of relatedness between strain J63 T and M. communis NBRC 102224 T , M. aquimarina CIP 108405 T and M. vaga JCM 20774 T were 56.2, 45.1 and 51.3 %, respectively. On the basis of the phenotypic, genetic and phylogenetic data, strain J63 T should be placed in the genus Marinomonas as representing a novel species, for which the name Marinomonas basaltis sp. nov. is proposed. The type strain is J63 T (5KCTC 22118 T 5JCM 14948 T ).

Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis

“…The phenotypic features of M. basaltis LMG 25279 T and M. communis LMG 2864 T were very similar, except for some features, namely that M. communis LMG 2864 T utilized sucrose, d -fructose, succinamic acid, urocanic acid and putrescine and had urease activity, whereas M. basaltis LMG 25279 T did not. Some phenotypic results for M. basaltis LMG 25279 T obtained in this study conflict with those reported by Chang et al (2008). They reported no growth in less than 1 % or more than 7 % NaCl and no esterase (C4), esterase lipase (C8) or naphthol-AS-BI-phosphohydrolase activities, but activities for trypsin and N -acetyl-β-glucosaminidase were present, and assimilation of l -arabinose, l -aspartic acid and glycerol.…”

“…DDH between M. basaltis LMG 25279 T and M. communis LMG 2864 T was above 70 % (78 %), which suggests that these species are synonymous. Chang et al (2008) obtained 56.2 % DDH between the same pair of type strains, but additional data from the present study (see below) support our value of 78 %. The authenticity of M. basaltis LMG 25279 T and M. communis LMG 2864 T used in this study was verified by 16S rRNA gene sequencing.…”

“…Marinomonas ostreistagni (Lau et al , 2006) and some M. aquimarina strains (Macián et al , 2005) were isolated from oysters. Marinomonas basaltis (Chang et al , 2008) and Marinomonas arenicola (Romanenko et al , 2009) were isolated from marine sediment, while Marinomonas balearica and Marinomonas pollencensis (Espinosa et al , 2010) were isolated from seagrass Posidonia oceanica .…”

Marinomonas alcarazii sp. nov., M. rhizomae sp. nov., M. foliarum sp. nov., M. posidonica sp. nov. and M. aquiplantarum sp. nov., isolated from the microbiota of the seagrass Posidonia oceanica

“…The number of species in this genus has increased during recent years, after the initial description of the genus by Van Landschoot & De Ley (1983). At the time of writing, the list of species with validly published names in the genus Marinomonas included M. communis and M. vaga (formerly Alteromonas communis and A. vaga ; Van Landschoot & De Ley, 1983; Baumann et al , 1972), M. mediterranea (Solano & Sanchez-Amat, 1999), M. primoryensis (Romanenko et al , 2003), M. pontica (Ivanova et al , 2005), M. dokdonensis (Yoon et al , 2005), M. aquimarina (Macián et al , 2005), M. ushuaiensis (Prabagaran et al , 2005), M. polaris (Gupta et al , 2006), M. ostreistagni (Lau et al , 2006), M. arctica (Zhang et al , 2008), M. basaltis (Chang et al , 2008), M. arenicola (Romanenko et al , 2009), M. balearica and M. pollencensis (Espinosa et al , 2010). M. brasilensis has since been added to the genus and M. communis and M. basaltis have been recognized as heterotypic synonyms (Chimetto et al ., 2011).…”

Marinomonas fungiae sp. nov., isolated from the coral Fungia echinata from the Andaman Sea

“…The DNA G+C content is 41–50 mol%. The genus Marinomonas belongs to the class Gammaproteobacteria , and it includes 22 species with validly published names at the time of writing, reported from various marine habitats: seawater from the Mediterranean Sea (Solano & Sanchez-Amat, 1999), subantarctic regions (Gupta et al , 2006; Prabagaran et al , 2005), the Black Sea (Ivanova et al , 2005), the East Sea of Korea (Yoon et al , 2005) and a pearl-oyster culture pond (Lau et al , 2006), coastal sea-ice of the Sea of Japan (Romanenko et al , 2003), the seagrass Posidonia oceanica (Espinosa et al , 2010), the coral Mussismilia hispida (Chimetto et al , 2009), oysters and seawater (Macián et al , 2005), marine sediment (Romanenko et al , 2009) and black sand (Chang et al , 2008).…”