Marinobacterium sedimentorum sp. nov., Isolated from the Bottom Sediments of the Okhotsk Sea

Abstract

A Gram-negative, aerobic, rod-shaped, motile bacterium designated КММ 9957T was isolated from a bottom sediment sample obtained from the Okhotsk Sea, Russia. Phylogenetic analyses based on the 16S rRNA gene and concatenated conserved protein-coding sequences positioned the novel strain KMM 9957T in the genus Marinobacterium as a distinct line adjacent to Marinobacterium rhizophilum CL-YJ9T, Marinobacterium profundum PAMC 27536T, and Marinobacterium aestuarii KCTC 52193T with 16S rRNA gene sequence similarities of 99%, 98.3%, and 98.2%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain KMM 9957T and M. aestuarii KCTC 52193T, M. profundum PAMC 27536T, and M. rhizophilum DSM 18822T were 89.4%, 87.9%, and 84.7% and 38.6%, 34.8%, and 28.4%, respectively. The genomic DNA G+C content of strain KMM 9957T was 58.4 mol%. The dominant respiratory quinone was ubiquinone Q-8, and the major fatty acids were C18:1, C10:0 3-OH, C16:0, and C16:1ω7c. The polar lipids of strain KMM 9957T consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, two unidentified aminolipids, eight unidentified phospholipids, and three unidentified lipids. Based on the combination of phylogenetic and phenotypic characteristics, strain KMM 9957T represents a novel species of the genus Marinobacterium, for which the name Marinobacterium sedimentorum sp. nov. is proposed.