Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., Isolated from Sea Water

Abstract

Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4°C and 40°C, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1 ω7c, C18:1 ω9c and C18:1 ω7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved protein-coding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1T ( = LMG 27497T = JCM 19399T = CIP 110588T = KMM 7502T) and Marinobacter similis sp. nov., with the type strain A3d10T ( = JCM 19398T = CIP 110589T = KMM 7501T), are proposed.

Marinobacter salinus sp. nov., a moderately halophilic bacterium isolated from a tidal flat environment

“…16S rRNA genes were amplified by PCR using a universal bacterial primer set 27F (5¢-AGAGTTTGATCMTGGCTCAG-3¢) and 1492R (5¢-TACGGYTACCTTGTTACGACTT-3¢) [13], and rpoD and secA genes were amplified using primers previously described (see [14,15]). Purified PCR products were sequenced by Macrogen using primers 27F, 338F, 786R and 1492R [13].…”

“…The two type strains M. sediminum R65 T (DSM 27079) and M. similis A3d10 T (DSM 15400) were selected as reference strains for comparison with the novel strains based on 16S rRNA gene sequence similarities and phylogenetic positions, and were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ). Unless stated otherwise, all reference strains were grown on MA or marine broth (MB) medium under optimal culture conditions [15,23].…”

Marinobacter adhaerens HP15 harbors two CzcCBA efflux pumps involved in zinc detoxification

“…A number of fully sequenced Marinobacter genomes is already available in public databases (e.g. Gauthier et al 1992, Ng et al, 2014, Song et al 2013. Thus a deeper bioinformatic analysis with focus on heavy metal resistance associated genes and gene clusters, including CzcCBA efflux pumps, will therefore be a future project.…”

Idiomarina atlantica sp. nov., a marine bacterium isolated from the deep sea sediment of the North Atlantic Ocean

“…Although the draft genome sequence was used to analyse the genotype to phenotype characteristic, significant correlation was also observed between the genotype to phenotype comparative analysis of phenotypic features retrieved from in vitro tests and from the draft genomes sequences in silico (Supplementary Table S1). However, there were some exceptions, maybe some repressor genes, regulatory proteins and global regulators prevent the expression of related genes under certain conditions, which was reported in some Vibrio species (Amaral et al 2014) and Marinobacter species (Ng et al 2014). Other characteristics are given in the species description (Table 1).…”