Marinobacter halodurans sp. nov., a halophilic bacterium isolated from sediment of a salt flat

Yoo, Yeonjae; Lee, Hanbyul; Kwon, Bong-Oh; Khim, Jong Seong; Baek, Seung-Woo; Pathiraja, Duleepa; Park, Byeonghyeok; Choi, In Geol; Kim, Gyu Hyeok; Kim, Beom Seok; Kim, Jae Jin

doi:10.1099/ijsem.0.004530

“…The morphological, biochemical and physiological properties of strain YJPS3-2 T were investigated by routine cultivation at 30 °C on YE/SW agar for 2 days. Gram-staining, anaerobic growth, oxidase and catalase activity, growth tests under different temperatures, pH and NaCl concentrations, hydrolysis of DNA, casein, hypoxanthine, starch, dl-tyrosine, xanthine, CM-cellulose, and chitin were tested as described previously [14,31]. Also, API 20NE and ZYM (bioMérieux), and GEN III MicroPlate system (Biolog) tests were performed to determine additional physiological and biochemical characteristics according to the manufacturer's instructions.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

Yoo

¹

,

Lee

²

,

Khim

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-negative, rod-shaped, cream-coloured, motile, halotolerant bacterium, designated as YJPS3-2T, was isolated from saltern sediment of the Yellow sea in Yongyu-do, Republic of Korea. Strain YJPS3-2T grew at pH 5.0–10.0 (optimum, pH 7.0), 4–40 °C (optimum, 30 °C) and with 1–15% (w/v) NaCl (optimum 3 %). The 16S rRNA gene sequence analysis indicated that strain YJPS3-2T was closely related to those of Halomonas halophila F5-7T (98.75 %), Halomonas salina F8-11T (98.74 %), Halomonas smyrnensis AAD6T (98.66 %), Halomonas organivorans G-16.1T (98.34 %), Halomonas koreensis SS20T (97.98 %) and Halomonas beimenensis NTU-107T (96.93 %). The average nucleotide identity and digital DNA–DNA hybridization values between YJPS3-2T and related type strains were 86.9–91.6 % and 32.0–44.8 %. Strain YJPS3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C16 : 0 (31.4 %), C19 : 0 ω8c cyclo (16.3 %), C17 : 0 cyclo (11.9 %) and C12 : 0 3-OH (10.4 %). The polar lipids consisted of phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain YJPS3-2T is 68.1mol %. Based on the polyphasic taxonomic evidence presented in this study, YJPS3-2T should be classified as representing a novel species within the genus Halmonas, for which name Halomonas getboli is proposed, with the type strain YJPS3-2T (= KCTC 92124T=KACC 22561T=JCM 35085T).

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“…The results related to growth range were recorded after incubation at 30 °C for 5 days. Hydrolysis capacity was carried out as described previously [33,34]. The results related to hydrolysis of substrates were recorded after incubation at 30 °C for 14 days.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Altererythrobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat and reclassification of Altererythrobacter deserti, Altererythrobacter estronivorus and Altererythrobacter muriae as Tsuneonella deserti comb. nov., Croceicoccus estronivorus comb. nov. and Alteripontixanthobacter muriae comb. nov.

Kim

¹

,

Yoo

²

,

Khim

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A yellow-coloured bacterium, designated strain JGD-16T, was isolated from a tidal flat in Janggu-do, Garorim Bay, Taean-gun, Chungcheongbuk-do, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-flagellated and short ovoid to coccoid-shaped. Growth was observed at 10–37 °C (optimum, 30 °C), pH 6.0–9.0 (pH 8.0) and with 1–5% (w/v) NaCl (2%). Results of 16S rRNA gene sequence analysis indicated that strain JGD-16T was closely related to Altererythrobacter xiamenensis LY02T (97.1 %), Altererythrobacter aurantiacus O30T (96.3 %), Altererythrobacter ishigakiensis JPCCMB0017T (95.8 %), Altererythrobacter epoxidivorans JCS350T (95.7 %) and Altererythrobacter insulae BPTF-M16T (95.3%). Phylogenomic analysis using the maximum-likelihood algorithm showed that strain JGD-16T formed a clade with the genus Altererythrobacter . The genomic DNA G+C content was 57.8 mol%. The predominant respiratory quinone was ubiquinone-10. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, a sphingoglycolipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were C18:1 ω7c (31.5 %) and C18:3 ω6c (19.6 %). On the basis of its phylogenomic, physiological and chemotaxonomical characteristics, strain JGD-16T represents a novel species within the genus Altererythrobacter , for which the name Altererythrobacter lutimaris JGD-16Tsp. nov. is proposed. The type strain is JGD-16T (=KCTC 72632T=KACC 21405T=JCM 33750T). We also propose the reclassification of Altererythrobacter deserti as Tsuneonella deserti comb. nov., Altererythrobacter estronivorus as Croceicoccus estronivorus comb. nov. and Altererythrobacter muriae as Alteripontixanthobacter muriae comb. nov.

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“…[5] with Marinobacter hydrocarbonoclasticus as the type species. At the time of writing, 57 species of the genus Marinobacter with validly published names have been isolated from different environments, such as sediments [6, 7], seawater [8–10], saline soil [11] and salt flat [12]. The aim of the present study was to determine the exact taxonomic position of strains PJ-16 T and PJ-38, which were isolated from a sea tidal flat.…”

Section: Introductionmentioning

confidence: 99%

Marinobacter panjinensis sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment

Yang

¹

,

An

²

,

He

³

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Two moderately halotolerant bacterium strains, designated PJ-16T and PJ-38, were isolated from a tidal flat of the red beach in Panjin City, Liaoning Province, PR China. Cells were found to be Gram-stain-negative, aerobic, motile, rod-shaped with a single polar flagellum. Optimum growth of strain PJ-16T occurred at 30 °C, pH 7.0 and 0.2–8.0 % (w/v) NaCl, and strain PJ-38 at 30 °C, pH 6.0–7.0 and 0.2–8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PJ-16T was most closely related to Marinobacter denitrificans KCTC 62941T (99.2 % 16S rRNA gene sequence similarity), Marinobacter algicola DSM 16394T (98.6 %), Marinobacter salarius JCM 19399T (98.4 %) and Marinobacter confluentis KCTC 42705T (98.2 %), and strain PJ-38 was most closely related to M. denitrificans KCTC 62941T (99.1 %), M. algicola DSM 16394T (98.6 %), M. salarius JCM 19399T (98.4 %) and M. confluentis KCTC 42705T (98.1 %). The G+C content of the genomic DNA of strain PJ-16T based on its draft genomic sequence was 57.4 mol%. The major cellular fatty acids of strain PJ-16T were C16 : 0, C16 : 1 ω7c/C16 : 1 ω6c and C18 : 1 ω9c. The major respiratory quinone of PJ-16T was ubiquinone-9 and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The results of the phenotypic, phylogenetic and genomic analyses revealed that strains PJ-16T and PJ-38 represent a novel species of the genus Marinobacter , and the name Marinobacter panjinensis sp. nov. is proposed. The type strain is PJ-16T (= CGMCC 1.13694T= KCTC 72023T).

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Marinobacter halodurans sp. nov., a halophilic bacterium isolated from sediment of a salt flat

Cited by 15 publications

References 36 publications

Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

Halomonas getboli sp. nov., a halotolerant bacteria isolated from a salt flat

Marinobacter panjinensis sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment

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