Marinobacter alexandrii sp. nov., a novel yellow-pigmented and algae growth-promoting bacterium isolated from marine phycosphere microbiota

Qiao, Yingjie; Feng, Qi; Zhang, Baoping; Gao, Jiajun; Zhi, Sheng; Xue, Qiping

doi:10.1007/s10482-021-01551-5

Cited by 27 publications

(24 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strain LZ-28 was isolated from toxic A. catenella LZT09 according to our protocol previously described [8–10]. The strain was purified and maintained on marine agar (MA; Difco), and preserved as a glycerol suspension (20%, v/v) at −80 °C for long term preservation, and was transferred into marine broth (MB) for experimental needs.…”

Section: Methodsmentioning

confidence: 99%

“…Gram-staining test was followed by the our procedures described previously [8–10]. Hanging-drop technique was used for observing motility during the exponential phase of culture growth.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, it’s also one key player to regulate the biosynthesis of paralytic shellfish poisoning toxins (PST) during various stages of harmful algal blooms (HAB) [5–7]. Within the phycosphere interface, exopolysaccharides (EPS) produced by bacterial consortia are the essential intermedia during their dynamic interactions [1,8–10].…”

Section: Introductionmentioning

confidence: 99%

“…To convey the composition of microbial consortium governing the phycosphere microbiota (PM) that are closely-associated with diverse HAB dinoflagellates, we have performed the Phycosphere Microbiome Project (PMP) [7–10,17–19]. During the investigation, we found that Mameliella is one predominant group and widely distributed in PM of various HAB dinoflagellates analyzed by metagenomics study (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, during our microbial cultivation study on diverse PM, a new versatile, rod-shaped, halophilic and red-pigmented bacterium designated as strain LZ-28 was isolated from highly-toxic HAB Alexandrium catenella LZT09. The new isolate produces active bioflocculanting EPS screened by our flocculanting assay [8–10]. Besides, it shows obvious microalgae growth-promoting ability to its host.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Comparative genomics reveals insights into phylogenomic taxonomy and potential algae-bacteria interactions of novel versatile Mameliella alba strain LZ-28 isolated from highly-toxic marine phycosphere

Zhu

Fang²,

Y³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Phycosphere harbors cross-kingdom interactions with significant ecological relevance for harmful algal blooms (HAB) and phycotoxins biosynthesis. Previously, a new red-pigmented bacterium designated as strain LZ-28 was isolated from phycosphere microbiota of typical HAB dinoflagellate Alexandrium catenella LZT09 which is a vitamin B 12 auxotroph and produces high levels of paralytic shellfish poisoning toxins (PST). Strain LZ-28 exhibited obvious growth-promoting activity toward its algal host, along with the production of active bioflocculanting exopolysaccharides (EPS). But the phylogenetic affiliation and genomic potential of this versatile bacterium has not yet been elucidated. In this study, we carried out combined taxonomic and phylogenomic analysis to clarify the taxonomic classification of strain LZ-28. The obtained 16S rRNA phylogeny revealed close taxonomic relationship between strain LZ-28 and other Mameliella alba members. Additional calculations of key phylogenomic parameters, average nucleotide identity (ANI), the average amino acid identity (AAI) and the digital DNA-DNA hybridization (dDDH) values based on genomes of strain LZ-28 and type strain of Mameliella alba were all exceeded the limit of species circumscription. Collectively considering the phenotypic and biochemical characterizations, strain LZ-28 was therefore identified as a new member of Mameliella alba. Furthermore, based on the genomic evidence, potential algae-bacteria interactions of strain LZ-28 with host algae LZT09 were elucidated through the associations with photosynthetic and antioxidant carotenoids, supplying of bacterial VB 12 to auxotroph host, and versatile EPS serving for bacterial colonization and nutrient exchange during their interactions, along with stress response systems to defense oxidative stress and quorum sensing (QS) signals benefited survival for bacteria in the symbiotic system. Comparative genomics shed light on similar genomic features between M . alba strains, revealed potential close associations of strain LZ-28 with its algae host, and further enriched the genomic repertoire of interactions between phycosphere microbiota and algal host LZT09.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Gram-staining test was followed by the our procedures described previously [8–10]. Hanging-drop technique was used for observing motility during the exponential phase of culture growth.…”

Section: Methodsmentioning

confidence: 99%