Marine yeast isolation and industrial application

Zaky, Abdelrahman Saleh; Tucker, Gregory A.; Daw, Zakaria Yehia; Du, Chenyu

doi:10.1111/1567-1364.12158

Cited by 106 publications

(79 citation statements)

References 77 publications

(119 reference statements)

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“…Isolation of yeasts was carried out by using standard methodology described by Tikka et al (2013) and Zaky et al (2014). After incubation, colonies were selected based on morphology and sub-cultured on yeast extract peptone dextrose (YPD) agar slants for further observations.…”

Section: Methodsmentioning

confidence: 99%

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

et al. 2017

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The purpose of this study is to evaluate the probiotic characteristics of 15 yeast strains isolated from nectar of toddy palm. Initially, the collected samples were inoculated on yeast extract peptone dextrose agar plates and the colonies so obtained were culturally and morphologically characterized. Commercial probiotic yeast, Saccharomyces boulardii served as the control in these experiments. Of the 15 yeast strains, the isolates that were resistant to antibiotics and worked synergistically with other cultures were considered for further evaluation. Selected isolates were evaluated in vitro for tolerance to simulated gastrointestinal conditions such as temperature, pH, bile and gastric juice. Further the yeast isolates were evaluated for their pathogenicity and adherence to intestinal epithelial cells. The 2 yeast isolates with efficient probiotic properties were finally characterized by sequencing their 5.8 S rRNA and partial sequences of internal transcribed spacer 1 and 2. The sequences were BLAST searched in the National Center for Biotechnology Information, nucleic acid database for sequence similarity of organisms and phylogenetic evolutionary analysis was carried out. Based on maximum similarity of basic local alignment search tool results, organisms were characterized as Pichia kudriavzevii OBS1 (100%) and Saccharomyces cerevisiae OBS2 (96%) and sequences were finally deposited in the GenBank data library. Among these two isolates, S. cerevisiae OBS2 displayed slight/moderate antioxidant and anticancer property. Hence, strain OBS2 can be utilized and explored as a potential probiotic for therapeutic applications.

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Section: Methodsmentioning

confidence: 99%

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

et al. 2017

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“…Different saccharides such as glucose, galactose, xylose, mannitol, laminaran, and arabinose are the common substrates used by ethanol and ABE fermenters Wei et al, 2013;Zaky et al, 2014). Nonetheless, a lower temperature, diluted acid, and shorter reaction time are often enough, thereby making the saccharification process of seaweed cheaper than the other feedstock.…”

Section: Fermentationmentioning

confidence: 99%

Seaweeds: a sustainable fuel source

Marquez

Santiañez

Trono

et al. 2015

Seaweed Sustainability

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“…The global market for industrial enzymes is now well established reaching around $5.0 billion in 2016 with a potential for growth rate of 4.7% until 2021 (Dewan, 2017). With several distinctive and promising attributes (Zaky et al, 2014), marine yeasts produce various enzyme such as inulinase, amylase, cellulase, exo β-1,3-glucanase, and protease (Liu et al, 2008;Zhang et al, 2009;Ni et al, 2009;Peng et al, 2011;Xu et al, 2012;Zhang et al, 2015;Rong et al, 2015 andChi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Crude Alkaline Extracellular Protease of Yarrowia lipolytica YlTun15

Bessadok¹,

Masri²,

Breuck³

et al. 2017

IPFS

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Abstract:Yarrowia lipolytica YlTun15 (GeneBank Acc. N° MF327143), isolated from farmed Dicentrarchus labrax's gills, secretes an alkaline extracellular protease, which exhibited the highest activity at pH 9 and temperature 45°C. The enzyme activity extracted was tested in the presence of different ion metal and protein inhibitors. .48% respectively at the 5 mM level. The enzyme was almost (activity=1.47%) inhibited by phenylmethylsulfonyl fluoride at the concentration of 5 mM. However, the protease activity was relatively constant in the presence of EDTA and SDS that may conclude that this enzyme was not a metalloprotease and belong to the serine protease category. After 18 months-storage at -20°C, the enzyme activity has decreased to 23.17%. This protease may have a potential application in food and detergent activity.

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Marine yeast isolation and industrial application

Cited by 106 publications

References 77 publications

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

Seaweeds: a sustainable fuel source

Characterization of the Crude Alkaline Extracellular Protease of Yarrowia lipolytica YlTun15

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