Marine sponge microbial association: Towards disclosing unique symbiotic interactions

Kiran, George Seghal; Sekar, Sivasankari; Ramasamy, P.; Thinesh, Thangadurai; Hassan, Saqib; Lipton, Anuj Nishanth; Ninawe, A S; Selvin, Joseph

doi:10.1016/j.marenvres.2018.04.017

Cited by 45 publications

(35 citation statements)

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“…The symbiosis between marine sponges and microorganisms is of considerable interest, both biologically and chemically [1,2]. Sponges are benthic organisms that have been colonizing different marine ecosystems, including coral reefs, for 600 million years [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…Their survival under drastically changing conditions requires a variety of adaptations, including the evolving strategy of symbiosis with beneficial microorganisms, which has been taking place since the Precambrian Age [5]. The mutualism between marine sponges and microbial symbionts is mainly related to nutrition and defense [1,6], under the control of dedicated enzymes and active secondary metabolites [2]. Although sponges are the main source of bioactive molecules isolated from marine organisms, a certain amount of evidence indicates that they are biosynthesized by microbial symbionts [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa

et al. 2019

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The fungi Chrysosporium lobatum TM-237-S5 was isolated from the sponge Acanthella cavernosa, collected from the mesophotic coral ecosystem of the Red Sea. The strain was cultivated on a potato dextrose agar (PDA) medium, coupling solid-state fermentation and solid-state extraction (SSF/SSE) with a neutral macroreticular polymeric adsorbent XAD Amberlite resin (AMBERLITE XAD1600N). The SSF/SSE lead to high chemodiversity and productivity compared to classical submerged cultivation. Ten phenalenone related compounds were isolated and fully characterized by one-dimensional and two-dimensional NMR and HRMS. Among them, four were found to be new compounds corresponding to isoconiolactone, (-)-peniciphenalenin F, (+)-8-hydroxyscleroderodin, and (+)-8-hydroxysclerodin. It is concluded that SSF/SSE is a powerful strategy, opening a new era for the exploitation of microbial secondary metabolites.the massive presence of microorganisms in the mesophyl matrix of the sponges, representing around 50% of their biomass [9][10][11].Fungi in the marine environment, and especially those associated with marine invertebrates, have been extensively investigated and reviewed [12,13]. A 2019 collaborative review highlighted the present state of knowledge and raised a multitude of open questions regarding the diversity and function of fungi in marine ecosystems [13].The symbiont assemblages inside the sponge are well organized in biofilms or dense colonies and are stabilized in the skeleton network over time [14,15]. This certainly impacts their development steps and the expression of biosynthetic clusters of secondary metabolites because it is now well documented that, in fungi, secondary metabolism and life cycle among fungi are co-regulated at the genomic level [16][17][18].This idea drives us to compare the metabolic profile of fungi cultivated on a gar slants and in liquid state. The result is that solid-state cultivation often leads to larger molecular diversity than classical liquid state fermentation LSF [19][20][21]. The major obstacle that stands against agar cultivation is the scale-up. In order to overcome such a challenge, we have developed specific innovative technologies, namely Platotex [22,23] and, more recently, Unifertex [24]. As we systematically coupled the culture of microorganisms with in-situ solid phase extraction (SPE), we also developed a specific SPE procedure for agar cultivation, termed solid-solid extraction (SSE) [25].In the present study, we report the impact of agar-supported cultivation on the production of secondary metabolites by the marine fungi Chrysosporium lobatum TM-237-S5, isolated from the Red Sea sponge Acanthella cavernosa. Chrysosporium lobatum was previously reported in the literature as a mosquito pathogenic fungus [26]. However, a very limited number of secondary metabolites have been reported in the literature for the genus Chrysosporium. Thus, the strain Chrysosporium queenslandicum IFM produced naphthaquinone-type altersolanols A, B, and C, the antifungal queenslandon, a representative...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa

et al. 2019

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“…The advances in sequencing technologies have provided large amounts of information on the genetic potential, as well as expressed functions of sponge-associated microbes Kiran et al, 2018). Through the advance of analysis methods and the integration of metagenomics with metatranscriptomics and metaproteomics (Chaib De Mares, 2018), a more and more complete and refined picture of microbial metabolism and possible interactions with other microbes or the sponge host can be drawn.…”

Section: Future Avenues For Cultivating Sponge-associated Microorganismsmentioning

confidence: 99%

“…In consequence, an anaerobic world has been discovered in high-microbial abundance sponges, which are often characterized by dense body tissue (Hoffmann et al, 2005(Hoffmann et al, , 2008 and numerous anaerobic microbial metabolic pathways have been described using multi-omics approaches, such as denitrification and sulfate reduction (Taylor, Radax, et al, 2007;Thomas et al, 2010;. Additionally, the phylogeny and genomic features of numerous sponge-associated microorganisms point towards an anaerobic, or at least facultative anaerobic lifestyle (Hentschel et al, 2006;Lavy et al, 2014;Alex & Antunes, 2018;Kiran et al, 2018). Strictly anaerobic cultivation led to the first and only report on the isolation of a sponge-associated member of the Chloroflexi (Brück et al, 2010).…”

Section: Anaerobic and Autotrophic Conditionsmentioning

confidence: 99%

“…The knowledge on symbiont functions remains limited to date which is partly due to the fact that all of the highly abundant sponge symbionts remain recalcitrant to cultivation. Recent advances in multi-omics techniques however have revealed genetic features of sponge-associated microorganisms that facilitate their symbiotic lifestyle and contribute to the sponge metabolism Astudillo-García et al, 2018;Bayer et al, 2018;Karimi et al, 2018;Kiran et al, 2018;Podell et al, 2019). Some of these processes are illustrated in Figure 5, 1-5, and a few selected symbiotic taxa and their functions are discussed below.…”

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confidence: 99%

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Ecological and biotechnological aspects of Aplysina-associated microorganisms

Gutleben¹

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Marine sponges are a prolific source of novel enzymes with promising biotechnological potential. Especially halogenases, which are key enzymes in the biosynthesis of brominated and chlorinated secondary metabolites, possess interesting properties towards the production of pharmaceuticals that are often halogenated. In this study we used a PCR-based screening to simultaneously examine and compare the richness and diversity of putative tryptophan halogenase protein sequences and bacterial community structures of six Aplysina species from the Mediterranean and Caribbean seas. At the phylum level bacterial community composition was similar amongst all investigated species and predominated by Actinobacteria, Chloroflexi, Cyanobacteria, Gemmatimonadetes, and Proteobacteria. We detected four phylogenetically diverse clades of putative tryptophan halogenase protein sequences, which were only distantly related to previously reported halogenases. The Mediterranean species A. aerophoba harbored unique halogenase sequences, of which the most predominant was related to a sponge-associated Psychrobacter-derived sequence. In contrast, the Caribbean species shared numerous novel halogenase sequence variants and exhibited a highly similar bacterial community composition at the OTU level. Correlations of relative abundances of halogenases with those of bacterial taxa suggest that prominent sponge symbiotic bacteria including Chloroflexi and Actinobacteria are putative producers of the detected enzymes and may thus contribute to the chemical defense of their host.

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Antibiofilm Metabolites from Sponge-Derived Aspergillus, Penicillium, and Fusarium for the Antibiotic Pipeline

Edrada-Ebel,

Michael,

Alsaleh

et al. 2024

Fungi Bioactive Metabolites

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Marine sponge microbial association: Towards disclosing unique symbiotic interactions

Cited by 45 publications

References 147 publications

Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa

Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa

Ecological and biotechnological aspects of Aplysina-associated microorganisms

Antibiofilm Metabolites from Sponge-Derived Aspergillus, Penicillium, and Fusarium for the Antibiotic Pipeline

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